Fecal source tracking by antibiotic resistance analysis on a watershed exhibiting low resistance

The ongoing development of microbial source tracking has made it possible to identify contamination sources with varying accuracy, depending on the method used. The purpose of this study was to test the efficiency of the antibiotic resistance analysis (ARA) method under low resistance by tracking the fecal sources at Turkey Creek, Oklahoma exhibiting this condition. The resistance patterns of 772 water-isolates, tested with nine antibiotics, were analyzed by discriminant analysis (DA) utilizing a five-source library containing 2250 isolates. The library passed various representativeness tests; however, two of the pulled-sample tests suggested insufficient sampling. The resubstitution test of the library individual sources showed significant isolate misclassification with an average rate of correct classification (ARCC) of 58%. These misclassifications were explained by low antibiotic resistance (Wilcoxon test P < 0.0001). Seasonal DA of stream E. coli isolates for the pooled sources human/livestock/deer indicated that in fall, the human source dominated (P < 0.0001) at a rate of 56%, and that human and livestock respective contributions in winter (35 and 39%), spring (43 and 40%), and summer (37 and 35%) were similar. Deer scored lower (17–28%) than human and livestock at every season. The DA was revised using results from a misclassification analysis to provide a perspective of the effect caused by low antibiotic resistance and a more realistic determination of the fecal source rates at Turkey Creek. The revision increased livestock rates by 13–14% (0.04 ≤ P ≤ 0.06), and decreased human and deer by 6–7%. Negative misclassification into livestock was significant (0.04 ≤ P ≤ 0.06). Low antibiotic resistance showed the greatest effect in this category.     

Modeling Parent and Metabolite Fate and Transport in Subsurface Drained Fields With Directly Connected Macropores1

Abstract: Few studies exist that evaluate or apply pesticide transport models based on measured parent and metabolite concentrations in fields with subsurface drainage. Furthermore, recent research suggests pesticide transport through exceedingly efficient direct connections, which occur when macropores are hydrologically connected to subsurface drains, but this connectivity has been simulated at only one field site in Allen County, Indiana. This research evaluates the Root Zone Water Quality Model (RZWQM) in simulating the transport of a parent compound and its metabolite at two subsurface drained field sites. Previous research used one of the field sites to test the original modification of the RZWQM to simulate directly connected macropores for bromide and the parent compound, but not for the metabolite. This research will evaluate RZWQM for parent/metabolite transformation and transport at this first field site, along with evaluating the model at an additional field site to evaluate whether the parameters for direct connectivity are transferable and whether model performance is consistent for the two field sites with unique soil, hydrologic, and environmental conditions. Isoxaflutole, the active ingredient in BALANCE^® herbicide, was applied to both fields. Isoxaflutole rapidly degrades into a metabolite (RPA 202248). This research used calibrated RZWQM models for each field based on observed subsurface drain flow and/or edge of field conservative tracer concentrations in subsurface flow. The calibrated models for both field sites required a portion (approximately 2% but this fraction may require calibration) of the available water and chemical in macropore flow to be routed directly into the subsurface drains to simulate peak concentrations in edge of field subsurface drain flow shortly after chemical applications. Confirming the results from the first field site, the existing modification for directly connected macropores continually failed to predict pesticide concentrations on the recession limbs of drainage hydrographs, suggesting that the current strategy only partially accounts for direct connectivity. Thirty‐year distributions of annual mass (drainage) loss of parent and metabolite in terms of percent of isoxaflutole applied suggested annual simulated percent losses of parent and metabolite (3.04 and 1.31%) no greater in drainage than losses in runoff on nondrained fields as reported in the literature.     

Biological Activity in a Heavily Organohalogen-Contaminated River Sediment (8 pp)

Background, Aims and Scope Sediments of the Spittelwasser creek are highly polluted with organic compounds and heavy metals due to the discharge of untreated waste waters from the industrial region of Bitterfeld-Wolfen, Germany over the course of more than one century. However, relatively few data have been published about the chloroorganic contamination of the sediment. This paper reports on the content of different (chloro)organic compounds with special emphasis on polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/F), and chlorobenzenes. Existing concepts for the remediation of Spittelwasser sediment include the investigation of natural attenuation processes, which largely depend on the presence of an intact microbial food web. In order to gain more insight in terms of biological activity, we analyzed the capacity of sediment microflora to degrade organic matter by measuring the activities of extracellular hydrolytic enzymes involved in the biogeochemical cycling of carbon, nitrogen, phosphorus and sulfur. Furthermore, the detection of physiologically active bacteria in the sediment, particularly of those known for their capability to reductively dehalogenate organochlorine compounds, illustrates the potential for intrinsic bioremediation processes. Methods PCDD/F and chlorobenzenes were analyzed by gas chromatography(GC)/mass spectrometry and GC/flame ionization detection, respectively. The activities of hydrolytic enzymes were determined from freshly sampled sediment layers using 4-methylumbelliferyl (MUF) or 7-amino-4-methylcoumarin-conjugated model compounds and kinetic fluorescence measurements. Physiologically active bacteria from different sediment layers were microscopically visualized by fluorescence in situ hybridization (FISH). Specific bacteria were identified by 16S rRNA gene amplification and sequencing. Results and Discussion The PCDD/F congener profile was dominated by dibenzofurans. In addition, the presence of specific tetra and pentachlorinated dibenzofurans supported the assumption that extensive magnesium production was one possible source for the high contamination. A range of other chloroorganic compounds, including several isomers of chlorobenzenes, hexachlorocyclohexane and 1,1,1-trichloro-2,2-bis (p-chloro-phenyl)ethane (DDT), was present in the sediment. Activities of extracellular hydrolytic enzymes showed a strong decrease in those sediment layers that were characterized by high contents of absorbable organic halogen (AOX), indicating disturbed organic matter decay. Interestingly, an abnormal increase of cellulolytic enzyme activities below the organochlorine-rich layers was observed, possibly caused by residual cellulose from discharges of sulfite pulping wastes. FISH revealed physiologically active bacteria in most sediment layers from the surface down to the depth of about 60 cm, including members of Desulfitobacterium (D.) and Sulfurospirillum. The presence of D. dehalogenans was confirmed by its partial 16S rRNA gene sequence. Conclusions Results of chemical sediment analyses demonstrated high loads of organochlorine compounds, particularly of PCDD/F. Several years after stopping the waste water discharge to Spittelwasser creek, this sediment remains a main source for pollution of the downstream river system by way of the ongoing mobilization of sediment during high floods. As indicated by our enzyme activity measurements, the decomposition potential for organic matter is low in organochlorine-rich sediment layers. In contrast, the comparably higher enzyme activities in less organochlorine-polluted sediment layers as well as the presence of physiologically active bacteria suggest a considerable potential for natural attenuation. Recommendations and Perspectives From our data we strongly recommend to explore the degradative capacity of sediment microorganisms and the limits for in situ activity towards specific sediment pollutants in more detail. This will give a sound basis for the integration of bioremediation approaches into general concepts to reduce the risk that permanently radiates from this highly contaminated sediment. Submission Editor: Dr. Henner Hollert (Henner.Hollert@urz.uniheidelberg.de)     

Near Real-Time Monitoring and Mapping of Specific Conductivity Levels Across Lake Texoma, USA

A submersible sonde equipped with a specific conductivity probe, linked with a global positioning satellite receiver was developed, deployed on a small boat, and used to map spatial and temporal variations in specific conductivity in a large reservoir. 7,695 sample points were recorded during 8 sampling trips. Specific conductivity ranged from 442,uS/cm to 3,378,uS/cm over the nine-month study. The data showed five statistically different zones in the reservoir: 2 different riverine zones, 2 different riverine transition zones, and a lacustrine zone (the main lake zone). These data were imported to a geographic information system where they were spatially interpolated to generate 8 maps showing specific conductivity levels across the entire surface of the lake. The highly dynamic nature of water quality, due to the widely differing nature of the rivers that flow into the reservoir and the effect of large inflows of fresh water during winter storms is easily captured and visualized using this approach.     

草原旅游发展中旅游管理部门环境意识的调查研究

根据环境意识结构．设计了旅游管理部门环境意识调查问卷。其主要的指标体系包括：旅游环境知识水平、旅游环境态度、旅游环境评价和旅游环境行为四个方面。对指标体系各部分之间以及每一指标部分的每一题目之间的相对重要性进行权重的确定．而且对各部分的每道题目的各个选项予以赋分。基于权重和赋分．结合调查问卷的实际情况，设计了指标体系各部分评价模型和环境意识总体评价模型。以此来计算草原旅游发展中旅游管理部门环境意识水平的综合得分。经过分析得知，旅游管理部门环境意识模式是“环境知识制约型”模式。     

Making Capacity Building Meaningful: A Framework for Strategic Action

This paper aims to give practical meaning to ‘capacity building’ through (a) identifying a suite of practical measures, such as mentoring or best practice guidelines, that have been shown to or are considered to build human, social, institutional, and economic capital; (b) placing these measures within a broader systems framework; and (c) exploring stakeholder feedback on specific measures to inform framework implementation. The 29 measures described provide actors, whether government or nongovernment, with a suite of practical investment choices for building capacity. These measures are then clustered into eight groups according to their primary purpose and placed within a systems framework. The framework provides a tool for actors with responsibilities for or an interest in capacity building to inform more holistic and strategic targeting of effort and investment. Stakeholder feedback gathered through surveys and workshops is subsequently reported to further inform implementation of specific measures within the framework’s eight groupings. The framework presented may be built upon through the identification and inclusion of further capacity building measures. The research is conducted within the context of decentralized governance arrangements for natural resource management (NRM), with specific focus on Australia’s recently formalized 56 NRM regions and their community-based governing boards as an informative arena of learning. Application of the framework is explored in the Australian setting through the identification and comparison of measures supported and most preferred by four major stakeholder groups, namely board members, regional NRM organization staff, policy/research interests, and Indigenous interests. The research also examines stakeholder perceptions of capacity issues, and whether these issues are likely to be addressed through implementing their preferred measures.

黄孢原毛平革菌处理染料废水过程中细胞结构损伤效应研究

利用透射电子显微镜观察分析白腐真菌(黄孢原毛平革菌)在处理染料废水活性艳红X-3B过程中,染料和盐度对黄孢原毛平革菌的细胞结构产生的毒性作用.结果表明:活性艳红X-3B染料对黄孢原毛平革菌产生生物毒性作用,且随着染料浓度的增加,细胞受损伤程度不断加深.加入100 mg/L的染料活性艳红X-3B后,黄孢原毛平革菌菌丝细胞形态发生变化,出现质壁分离现象;染料浓度进一步加大,菌丝细胞超微结构受到损伤逐渐严重.染料废水中的盐度对黄孢原毛平革菌细胞也会造成损伤,且损伤程度随盐度增大而增大.NaCl浓度为3 g/L时,菌丝细胞发生质壁分离;而当NaCl加入量高于8 g/L时,细胞膜受损,线粒体、细胞核呈现空泡化,表现为受到不可逆的损伤.染料和盐双因子对黄孢原毛平革菌细胞的损伤效应表现为其损伤程度与单因子作用一致,且染料的影响作用占主导.     

生态土壤渗滤系统启动周期研究

利用室内模拟试验装置,考察了4种生态土壤渗滤系统在0.1 m3/(m2·d)的水力负荷条件下对生活污水中TP、COD和NH3-N的去除效果及启动周期; 同时对整个系统及同类生态工艺启动周期的判断方法做了探讨.研究结果表明,生态土壤渗滤系统对TP、COD和NH3-N的启动周期分别为15～27 d、24～40 d和24～26 d; 土壤渗滤系统对TP的启动周期最短,对COD的启动周期最长; 处理系统启动周期的判断原则是综合考察系统对主要污染物各自的启动周期,以最长的作为系统启动周期.4组试验中,1#和2#系统的启动周期为40 d; 3#和4#的为24 d.     

Conjoint analysis for environmental evaluation

BACKGROUND, AIM AND SCOPE: Conjoint analysis and the related choice-modelling methods have been used for many years in marketing research to evaluate consumer behaviour and preferences for different kinds of product attributes. Recently, the number of applications in environmental science and management has started to grow. Conjoint analysis is found in many different forms, and the environmental studies evaluated in this review display the same range of methods as in other fields. The key characteristic of all these methods is that trade-offs are evaluated by jointly considering a number of important attributes. MAIN FEATURES: This paper is a review of the literature on environmental applications of conjoint analysis and assesses in which environmental area conjoint analysis has been most successful. The method and the design of the studies are reviewed as well. RESULTS: A total of 84 studies were found, dealing with environmental issues that were evaluated by conjoint analysis. The studies concern agriculture, ecosystem management, energy, environmental evaluation, forestry, land management, pollution, products, recreation, environmental risk analysis and waste management. DISCUSSION: Choice experiments seem to have a comparatively stronger position in environmental studies than elsewhere. Most of the environmental applications are related to natural resource management. This is somewhat surprising, but a number of reports have appeared also on product evaluation, which could be a key application area in the future. CONCLUSIONS: Compared to marketing and transportation, the number of environmental conjoint studies is rather small but increasing, and the method has proven to work effectively in eliciting preferences on environmental issues. In environmental issues, experimenters often use choice experiments, especially concerning ecosystem management and environmental evaluations. When it comes to evaluating preferences concerning agriculture, forestry, energy and products, a more traditional approach of conjoint analysis is favoured. RECOMMENDATIONS AND PERSPECTIVES: Two new areas of application are identified in this review--environmental communication and expert elicitation. Conjoint analysis can thus be developed into a useful instrument for environmental risk analysis and communication, both of which are necessary for an efficient approach to risk governance.     

浙江木材产业可持续发展分析

浙江是一个森林资源稀缺的区域,在面临森林资源稀缺的条件下,浙江木材产业发展却位居全国前列.论文首先从产业规模、产业组织和产业集聚三方面总续了浙江木材产业发展现状及特点;在此基础上,总结了浙江木材产业快速发展过程中面临的主要问题,诸如木材资源供给的不可持续性、木材产业经营成本不断上涨、国际市场与国内市场分割等.问题分析表明,木材资源的短缺已成为浙江木材产业发展的瓶颈,经营成本的上涨影响了浙江木材产业发展的动力,国内外市场分割限制了浙江木材产业的竞争范围,这直接影响到浙江木材产业的可持续发展.现代市场经济发展表明,产业可持续发展需要企业、行业协会以及政府的通力合作.最后,论文分别从企业、行业协会和政府三个层面提出了促进浙江木材产业可持续发展的对策建议.