NirS-type denitrifying bacteria in aerobic water layers of two drinking water reservoirs: Insights into the abundance, community diversity and co-existence model

The nirS-type denitrifying bacterial community is the main drivers of the nitrogen loss process in drinking water reservoir ecosystems.The temporal patterns in nirS gene abundance and nirS-type denitrifying bacterial community harbored in aerobic water layers of drinking water reservoirs have not been studied well.In this study,quantitative polymerase chain reaction (qPCR) and Illumina Miseq sequencing were employed to explore the nirS gene abundance and denitrifying bacterial community structur...     

Isolation and characterization of a strain with high microbial attachment in aerobic granular sludge

Aerobic granule is a special microbial aggregate associated with biofilm structure. The formation of aerobic granular sludge is primarily depending on its bacterial community and relevant microbiological properties. In this experiment, a strain with high microbial attachment was isolated from aerobic granular sludge, and the detailed characteristics were examined. Its high attachment ability could reach 2.34 (OD_600nm), while other low attachment values were only around 0.06-0.32, which indicated a big variation among the different bacteria. The strain exhibited a very special morphology with many fibric fingers under SEM observation. A distinctive behaviour was to form a spherical particle by themselves, which would be very beneficial for the formation and development of granular sludge. The EPS measurement showed that its PN content was higher than low attachment bacteria, and 3D-EEM confirmed that there were some different components. Based on the 16S rRNA analysis, it was identified to mostly belong to Stenotrophomonas. Its augmentation to particle sludge cultivation demonstrated that the strain could significantly promote the formation of aerobic granule. Conclusively, it was strongly suggested that it might be used as a good and potential model strain or chassis organism for the aerobic granular sludge formation and development.     

升流式厌氧污泥床--压力式好氧接触氧化工艺处理制药废水的实践

采用升流式厌氧污泥床—压力式好氧接触氧化工艺处理锦州九洋药业有限责任公司制药废水,该工艺技术成熟、处理效率高,运行稳定可靠。结果表明处理后的出水水质可以满足排放标准的要求。     

载体好氧预挂膜处理对厌氧生物颗粒性能的影响

研究了生物膜载体经好氧预挂膜处理对厌氧附着膜膨胀床(AAFEB)反应器中生物颗粒性能的影响.载体经10 d好氧预挂膜处理后,形成了7～8 μm厚的生物膜,在起动运行过程中,处理反应器中载体的挂膜进程比对照要快.在整个运试期间,处理反应器中的生物膜厚度和污泥浓度值均高于对照.前者厌氧生物颗粒致密、结实,生物膜结构良好,滞留于反应器中的性能较佳,比后者较不宜随水流失.处理反应器厌氧生物颗粒的一些性能指标值,如最大比产酸活性、最大比产甲烷活性、脱氢酶活性、辅酶F₄₂₀含量等均高于对照.另外,厌氧生物颗粒内微生物相也有明显的差异.      

The influence of storage on the morphology and physiology of phthalic acid-degrading aerobic granules

The precultured aerobic granules with special degradabilities could be used as a feasible bioseed for enhancement of aerobic granulation systems. In practice, the storage stability, physicochemical characteristics, and recovering efficiency of granules are crucial for a long-distance transportation and successful application. In this study, phthalic acid (PA)-degrading aerobic granules were cultivated and stored for 8 wk at 4 °C. The granular size, settling ability as well as structure integrity was found stable during the storage period. It was observed that the upper 1/3 part of granules stored in the reagent bottle turned to black color, while the lower 2/3 part granules did not significantly change color (brown–yellow) after the 8-wk storage. The black and brown–yellow color PA-degrading granules were manually separated and re-inoculated into two identical sequencing batch reactors for reviving the PA degradation capability. After a 7 d operation, both black and yellow granules restored their activities to the levels before storage, in terms of total organic carbon removal efficiency (100%), specific oxygen uptake rate (59 mg g VSS⁻¹ h⁻¹), and adenosine triphosphate content (0.016 mg g VSS⁻¹). This study demonstrated that aerobic granules grown on a complex substrate could tolerate storage conditions and rapidly restored their bioactivities toward the target pollutant. The results also shed the light on the future application of precultured aerobic granules with unique functions for biodegradation and bioremediation purpose.     

厌氧—好氧处理高得率浆APMP废水的研究

设计了生物处理高得率浆碱性过氧化氢机械浆 (APMP)废水厌氧 (UASB)—好氧 (SBR)组合技术 ,结果表明该技术可使 APMP废水 COD去除率达 90 .3 %。并用计算机处理实验结果 ,建立了厌氧—好氧关联方程及 SBR降解 APMP废水的动力学关系式。     

啤酒废水治理技术进展

简述了近年来啤酒废水处理技术研究及应用现状，这些方法包括：好氧法、厌氧－好氧法、厌氧法；特别介绍了一些新工艺：ＳＢＲ，ＡＢＦ，ＥＧＳＢ，ＩＣ等；并对啤酒废水处理技术的发展前景进行了展望。     

缺氧反硝化去除难降解杂环化合物吡啶研究

在实验室中，采用好氧、厌氧和缺氧间歇试验，研究焦化废水中一种难降解有机物--吡啶的生物降解性能。试验主要研究了在缺氧状态下吡啶的生物降解性能、适宜的N/C比、N源的变化情况等。研究结果表明，缺氧反硝化状态下吡啶的生物降解性能有显著的提高。     

载体好氧预挂膜处理对厌氧消化反应器启动的影响

研究了生物膜载体经好氧预挂膜处理对厌氧附着膜膨胀床反应器 (AAFEB)启动的影响。载体经10天好氧预挂膜处理后的反应器比对照提前 15天完成启动。载体经好氧预挂膜处理的反应器中生物膜比对照反应器较早出现厌氧丝状菌 ,而且占据优势 ,而对照反应器则以球状细菌较多。前者厌氧颗粒污泥致密、结实 ,生物膜结构良好 ,滞留于反应器中的性能较佳 ,比后者较不易随水流失     

高浓度有机氰废水的好氧可生化性研究

采用BODs／COD比值法和好氧呼吸曲线法在国内外首次针对高浓度有机氰废水及其污染物进行了全面的好氧可生化性的研究。结果表明，6种废水中，一段、二段急冷废水的可生化性能很差，很难用生化法进行处理I四效蒸发液，四效冷凝液可生化性较好适于生化处理，腈纶工艺废水在低浓度下，可以用生化法处理；高浓度有机氰废水中的9种污染物，丙烯腈、乙腈、丙烯酸、CN^-的好氧可生化性能较差，高浓度下对活性污泥有抑制作用，聚合物的可生化性能最差，根本无法被好氧生物降解。