硒酸钠和亚硒酸钠对长春花细胞悬浮生长的影响

研究了硒化合物对长春花(C.roseus)细胞悬浮生长的影响.在pH5.5培养介质中,当Se(Ⅵ)>0.5ppm或Se(Ⅳ)>0.6ppm时,将抑制C.roseus细胞悬浮生长,其致死浓度分别是100ppm Se(Ⅵ)和2.0ppm Se(Ⅳ).C.roseus对硒化合物有效强的富集作用.硒化合物的毒性和培养介质的pH值有关.在本试验条件下,硒酸钠和亚硒酸钠对Hg~(2+)无拮抗作用.     

Detection of both the normal and mutant alleles in single cells of individuals heterozygous for the sickle cell mutation—prelude to preimplantation diagnosis

As a preliminary step to preimplantation diagnosis of sickle cell disease in unfertilized eggs or 8-cell embryos of heterozygous parents, we established quality control for detection of the mutant and normal alleles of the beta-haemoglobin gene using single buccal cells. Efficient polymerase chain reaction (PCR) amplification of a 680 base pair sequence of the beta-globin gene spanning the site of the sickle cell mutation was obtained for 79 per cent of single heterozygous cells. In 71 per cent of cases, both alleles were detected. With this current efficiency, we predict that a clinical preimplantation diagnosis at the 8-cell embryo stage could be carried out safely and reliably for a couple at risk of transmitting sickle cell disease to their children.     

Erythroid-specific antibodies enhance detection of fetal nucleated erythrocytes in maternal blood

Fetal nucleated erythrocytes (NRBC) in maternal blood are a non-invasive source of fetal DNA for prenatal genetic screening. We compared the effectiveness of three monoclonal antibodies for the separation of fetal cells from maternal blood by flow sorting. Mononuclear blood cells from 49 healthy pregnant women were incubated with antibody to CD 71, CD 36, and/or glycophorin A (GPA), employed singly or in combination with each other. These monoclonal antibodies recognize surface antigens on haematopoietic precursor cells. Successful isolation of fetal cells was defined as detection of Y chromosomal sequences in maternal blood from women carrying male fetuses, with absence of Y sequences when female fetuses were carried. Thus, gender prediction accuracy was used as a measure of fetal cell separation. Using anti-CD 71 to isolate fetal cells, gender prediction was 57 per cent correct; with anti-CD 36, it was 88 per cent correct. Anti-GPA, an erythrocyte-specific antigen, used alone or in combination with anti-CD 71 or 36, improved gender prediction to 100 per cent. We conclude that antibody to GPA improves the retrieval of fetal NRBC from maternal blood, permitting genetic analysis by the polymerase chain reaction.     

北方庭院生态系统能量分析及养分平衡研究

以基于食用菌生产的北方庭院生态系统为研究对象，通过对沈阳南郊一典型农户庭院生态系统的能量分析与养分平衡研究表明，该庭院生态系统的产品总输出能为４１８７２１４ＭＪ／ａ，其中平菇产品的生物能占６５４％；输入输出系统的Ｎ、Ｐ２Ｏ５及Ｋ２Ｏ基本上保持着养分平衡；该庭院生态系统是一个生产力高、综合效益可观的系统，对指导该地区农村庭院生态系统建设具有重要的现实意义。     

Chorionic villus culture for prenatal diagnosis of chromosome defects: Reduction of the long-term cultivation time

We report in detail two series of chorionic villus cultivation for prenatal chromosomal diagnosis. Chorionic villi were sampled from both first- and second-trimester pregnancies. One hundred cultures were treated with trypsin–EDTA for 2 h and collagenase overnight, (method A) and 100 were treated with trypsin–EDTA for 1 h and collagenase for 2 h (method B). Using short-term enzymatic digestion, the cultivation time was reduced from 14 days to 6 days. Sufficient amounts of metaphases of good quality were present in 93 per cent of primary cultures harvested in situ, whereas enough metaphases of sufficiently good quality were in most cases present only after subcultivation of the cultures using method A. The decrease in cultivation time obtained is probably due to a higher yield of viable cells in monocellular suspension, an increased attachment efficiency, and a more rapid attachment of single cells (within 24 h).     

中国东南沿海中生代火山作用基本特征

中国东南沿海自晚侏罗世到白垩纪火山活动分为二期、四个旋回,在空间上有迁移性,在时间上与侵入活动呈交替式演变。建立火山碎屑流等五种岩相模式,岩浆爆发占主导地位,同时存在蒸气岩浆爆发。确定破火山口、火山穹隆和锥火山为火山构造基本类型。由火山作用与区域构造作用、沉积作用控制形成火山沉积洼地、火山构造洼地和火山构造隆起。提出不同类型火山构造组合形式和相互演化关系。火山岩系列属高钾钙碱系列,晚期则具双模式性质。它们的地球化学型式兼具大陆边缘和大陆板内双重特性。     

京津地区能源、大气污染物扩散对区域元素循环的作用

对京津区域１９８０－１９９２年间能源利用状况进行分析，并通过模式计算该区域大气污染物的扩散规律，结果表明，大气污染物随能源利用增加而增加，其增加速率ＮＯｘ大于ＯＳ２，经预测，至２００４年京津地区ＳＯ２排放量增咖１１．４０％，ＮＯｘ增加５６．８０％，由于气象和地形条件所致，该区域内不利于大气污染物远距离输送，这使箱型区域内物料平衡计算可以简化。     

炼油装置运行周期评估技术及应用

炼油装置运行周期评估技术通过统计、分析装置故障数据,计算特定时间段装置的可靠性,进而获得装置可靠性随时间的变化规律和装置可靠性最差设备排序。介绍了该技术在某常减压装置的应用,应用结果表明,该评估技术为科学确定炼油装置运行周期提供了决策依据。     

Integrated environmental assessment and pollution prevention in Vietnam: the case of anthracite production

Using the concept of life cycle analysis, the whole life cycle of anthracite production in Vietnam was assessed and the environmental “hot-spots” were identified. These include (i) dust pollution at coal transfer points or piles in storage; (ii) noise pollution resulting from the usage of heavy equipment, and (iii) high volume of acid and turbid mine water discharged into the environment.Pollution prevention and treatment options were identified and discussed in this study which consists of wet treatment of dust, planning, collection and treatment of mine water, planting of trees for dust and noise reduction as well as minimization of erosion. Some recommendations are made for pollution abatement from coal production and improvement of anthracite production in the region.     

基于行业全产业链评估一份外卖订单的环境影响

构建"生产-运输-使用-废弃处置"的外卖全产业链，通过大样本企业调研与外卖平台大数据分析，计算8个样本城市不同材质与规格的外卖餐盒、餐具、包装袋的市场使用比例，挖掘骑手配送的海量数据，收集城市垃圾处理处置情况，系统评估外卖全过程的环境影响.结果表明，外卖包装物全产业链中生产环节（45%）与废弃处置环节（50%）为环境影响的主要来源，严重程度依次为固废排放、水污染、资源能源消耗和大气污染.所在城市及选用包装物材质的不同，外卖行业造成的环境影响会有显著区别，全产业链上的各环节需相互配合，多主体共同承担环境责任，采取差别化的减缓应对措施.