酸性硫酸盐土鳗塘水体防酸技术

为了克服生产实际中用石灰调节鳗塘水体酸性的弊端,利用石灰石石屑防御酸性物质入侵,效果特别好。水体pH维持在7～8的恒定水平;相关溶解氧含量也适合鳗鱼生长的需要:水草生长茂盛;经济效益可观。     

Vibrational signals in a gregarious sawfly larva (<Emphasis Type="Italic">Perga affinis</Emphasis>): group coordination or competitive signaling?

Group living confers both benefits and costs to the individuals involved. Benefits may include enhanced defense, thermoregulation, and increased foraging efficiency while costs often involve competition for resources such as food, shelter, and mates. Communication provides a medium of exchange among individuals engaged in either cooperative or competitive interactions. The functional analysis of signals within groups therefore requires testing both cooperative and competitive functions, although the latter is infrequently done. In this paper, I study the use of two vibrational signals in a gregarious, processionary Australian sawfly larva, Perga affinis: tapping and contractions. Tapping involves striking the substrate with the sclerotized portion of the abdominal tail and a contraction is a fast, whole-body twitch, which is both tactile and vibrational in its transmission. For tapping, I first demonstrate that it is a form of communication, as tapping of one larva elicits tapping in another, and that it is transmitted through substrate vibrations. I then test whether the signal is mostly cooperative or competitive in nature by examining it in light of two hypotheses: (1) the Group Coordination hypothesis, stating that the signal functions to maintain group cohesiveness and (2) the Competitive Signaling hypothesis, stating that tapping serves as a competitive assessment signal between larvae while feeding. For contractions, I test only the group coordination hypothesis that they serve to coordinate and initiate group movement. Results support the group coordination hypothesis for each signal. While feeding, lone larvae (without potential competitors) were significantly more likely to tap than those in groups, and this trend continued in non-feeding situations. Contractions regularly preceded periods of group movement during processions and were given with increasing frequency before departure from preforaging clusters. The vibrational signals in this processionary species likely function cooperatively to maintain group cohesiveness and coordinate movement.     

Defence effectiveness of easy bleeding sawfly larvae towards invertebrate and avian predators

Summary. Easy bleeding is a phenomenon discovered in some tenthredinid insects which possess a particularly low mechanical resistance of the integument, leading under mechanical stress to haemolymph exudation. It has a defensive effect against ants and wasps through harmful plant compounds which are sequestered in the haemolymph. Here we describe etho-ecological and some chemical aspects of the defence of easy bleeders and specify the range of predators to which easy bleeding might be effective. Beside a high haemolymph deterrence associated with low integument resistance across sawfly species, we also detected toxicity of the haemolymph of some species to workers of the ant Myrmica rubra. The behaviour of easy bleeders is to move slowly and, once disturbed, to become motionless, thereby probably impeding the tendency of a predator to attack. This behaviour had no beneficial effect for easy bleeders when attacked by the predatory bug Podisus maculiventris. Bugs could successfully and without harm prey on sawfly larvae without evoking easy bleeding. For the easy bleeder Athalia rosae, host plants with different secondary metabolite profiles, and, consequently, changes in haemolymph chemistry only slightly affected the feeding behaviour of the bugs. To test the effectiveness of easy bleeding towards a vertebrate predator, easy bleeders were offered to birds, Sturnus vulgaris. The body colouration of the sawfly larvae was of prime importance in determining the predators response when testing birds in a group. It is likely that easy bleeding is a defence strategy directed primarily towards foraging insects with biting-chewing mandibles and that it is much less active towards predatory insects with piercing-sucking mandibles as well as birds. The involvement of chemical and/or physical cues in the strategy is discussed with respect to these types of predators.     

多氯代甲烷对鳝鱼红细胞微核率及核异常率的影响

试验采用外周血红细胞微核测试技术,通过分析鳝鱼外周血细胞的核异常率和微核率,研究多氯代甲烷的遗传毒性和生态安全性.结果表明:①96 h后,质量浓度为3 mg·L-,30 mg·L-1和300 mg·L-1的CCl4和CHCl3污染所产生的核异常率分别为:5.38%和4.26%,9.62%和6.50%,12.18%和9.54%,表明CCl4对细胞异常率的影响大于CHCl3对细胞核异常率的影响;②96 h后,质量浓度为3 mg·L-1,30 mg·L-1和300 mg·L-1的CCl4和CHCl3污染所产生的微核率分别为:0.64%和0.40%,0.94%和0.55%,1.31%和0.95%,说明CCl4对细胞微核率的影响大于CHCl3对细胞核微核率的影响.表明用微核测试的方法分析环境中的污染物对生物体危害的程度是一种最便捷和有效的手段.     

黑水虻转化厨余垃圾过程中病原菌灭活规律的研究与综合评价

黑水虻能够有效取食厨余垃圾等有机固体废弃物,其自身转化为昆虫蛋白和脂肪等生物质,将厨余垃圾转化为虫沙有机肥,处理过程是一种有机固体废弃物的资源化新方法.为评估黑水虻处理厨余垃圾的效率与安全性,本课题研究黑水虻在厨余垃圾处理过程中,对大肠杆菌O157：H7（EC）、鼠伤寒沙门氏菌（ST）及金黄色葡萄球（SA）的灭活能力,评价黑水虻体内的抑菌因子,分析3种病原菌对黑水虻生长增重及厨余转化效率的影响.经过18 d的黑水虻处理,研究发现：①第0和第6 d两次以6.4~7.1 log₁₀ CFU·g^-1的浓度向厨余垃圾中分别接种EC、ST和SA以后,EC经4~6 d处理被全部灭活、ST经3~4 d处理被全部灭活,SA经6 d处理,浓度下降到1.9~2.6 log₁₀ CFU·g^-1,但不能被全部灭活,病原菌的灭活效率呈现EC=ST>SA的趋势（p < 0.001）,且黑水虻体内无EC、ST或SA残留;②厨余垃圾降解过程中,初期和中期pH值主要呈现酸性（4.0~5.3）,对抑制EC、ST和SA起到了促进作用,后期pH值呈中性至弱碱性;③黑水虻对于EC和ST能够产生自身免疫抑制因子,且抑制活性ST>EC,但对SA无明显的免疫抑制能力,SA的灭活主要依赖于黑水虻肠道菌群的竞争性抑制作用;④黑水虻的体长、体重、预蛹率、产率,以及厨余垃圾的生物转化率和减量化率未受病原菌存在的影响,18 d后EC组、ST组和SA组的预蛹率均达到在80%以上,厨余减量化率分别达到74.0%、79.1%和78.5%,生物转化率分别达到13.0%、13.2%、19.4%.综上,黑水虻能够在彻底灭活EC、ST,99%灭活SA的同时,高效降解厨余垃圾（减量化率>74%）,并转化为虫体有机质（虫产率>10%）,是一种高效卫生的厨余垃圾资源化方法.今后的研究中,还需要加强以金葡菌为代表的抗逆性病原菌的灭活机制的探讨.     

Strong links between metal contamination, habitat modification and estuarine larval fish distributions

Changes to larval fish assemblages may have far reaching ecological impacts. Correlations between habitat modification, contamination and marine larval fish communities have rarely been assessed in situ. We investigated links between the large-scale distribution of stressors and larval fish assemblages in estuarine environments. Larval fish communities were sampled using a benthic sled within the inner and outer zones of three heavily modified and three relatively unmodified estuaries. Larval abundances were significantly greater in modified estuaries, and there were trends towards greater diversity in these systems. Differences in larval community composition were strongly related to sediment metal levels and reduced seagrass cover. The differences observed were driven by two abundant species, Paedogobius kimurai and Ambassis jacksoniensis, which occurred in large numbers almost exclusively in highly contaminated and pristine locations respectively. These findings suggest that contamination and habitat alteration manifest in substantial differences in the composition of estuarine larval fish assemblages.     

Seaweed secondary metabolites as antifoulants: effects of Dictyota spp. diterpenes on survivorship, settlement, and development of marine invertebrate larvae

Summary. A recent investigation showed that the brown seaweed Dictyota menstrualis was unfouled relative to co-occurring seaweeds, and that larvae of fouling invertebrates avoided settling on D. menstrualis due to chemicals on its surface. The secondary metabolites dictyol E and pachydictyol A are among the compounds found on this alga's surface. In the present study, we tested the effects of specific diterpenes from Dictyota on the survivorship, growth, and development of invertebrate larvae and developing juveniles that could foul seaweeds. Exposure to dictyol E, dictyol B acetate, pachydictyol A, and dictyodial from Dictyota menstrualis and D. ciliolata caused significant larval mortality, abnormal development, and reduce growth rates for three species of co-occurring invertebrates when their larvae were forced into contact with these metabolites. Larvae were damaged at metabolite concentrations as low as 5% of maximum possible surface concentrations of these compounds for the populations of Dictyota we studied. The negative effects of these secondary metabolites on potential foulers, in conjunction with data demonstrating larval avoidance of dictyol-covered surfaces, suggest that these compounds could function as chemical defenses against fouling, and could select for larvae that avoid hosts producing these metabolites. Received 25 May 1998; accepted 22 June 1998.     

Metabolic effects of diazinon on the European eel

Abstract

Eels were exposed to a sublethal diazinon concentration of 0.042 mg/L for exposure times of 6, 24, 48, 72 and 96 hours. Biochemical analyses of blood composition, such as plasma glucose, total plasma cholesterol and triglycerides, plasma lactate, plasma urea and uric acid, showed significant differences between treated and control animals. Plasma glucose and lactate increased after 6 hours exposure to the insecticide. Plasma cholesterol and triglycerides content decreased during 96 hours treatment. Urea levels increased at 72 hours while uric acid content decreased significantly at 24, 72 and 96 hours exposure to the pesticide.

The observed effects of diazinon on eel metabolism suggested that the treated fish was faced with a serious metabolic crisis, and the fish looked for alternative methods of metabolism to overcome the toxic stress.     

Pilot-scale study of removal effect on Chironomid larvae with chlorine dioxide

Introduction C hironom id, from the dipteran fam ily Chirono- m idae, is a non-biting m idge that is w idely distributed in the northern hem isphere at tem perate latitudes. C hironom id larvae can be found both in lentic and lotic environm ents, usually …