江西省气候变化的特征及其对水稻产量的贡献——基于“气候-经济”模型

采用气候倾向率和M-K突变检验,分析1951~2012年江西省气候和温度的变化趋势和突变特征;构建"气候-经济"新模型,研究气候因子对水稻产量的影响,最后借助BP神经网络模型预测未来10a气候因子对水稻产量的贡献。结果表明:过去60多年来气温平均每10a升高0.172℃,而降水的线性倾向不显著;气温突变开始的时间约为1997年,降水的突变趋势不明显;不同阶段气候因素的贡献率及作用方向存在差异,1978~1997年气候因子贡献率为5.97%,其中温度和降水贡献率分别为1.51%、4.46%。1998~2013年气候因子贡献率为8.98%,温度和降水贡献率分别为4.63%、4.35%。物质要素在不同阶段的贡献率均为70%以上,其中播种面积和化肥的影响程度较大;未来10a气温对江西水稻产量的贡献为1.48,降水量对水稻产量的贡献为2.22。     

丙烯醛-DNA加合物的研究进展

丙烯醛是一种活泼的a,β不饱和醛,在环境中广泛存在,香烟烟气和厨房油烟是人体丙烯醛暴露的主要环境来源.另一方面机体内丙烯醛可以通过脂质过氧化、氨基酸氧化等多种途径自发生成.进入人体后,丙烯醛和DNA发生加合生成丙烯醛-DNA加合物,目前研究最多的是丙烯醛-dG加合物,包括a-OH-PdG和γ-OH-PdG,其中γ-OH...     

1956~2011年鄱阳湖水沙特征及其变化规律分析

近年来,鄱阳湖水沙研究集中在鄱阳湖"五河"各水文站的单站分析上,且水沙数据采用大多仅至21世纪初期,对缺测资料未进行有效插补。将鄱阳湖作为一个研究整体,分析了鄱阳湖水沙特征及其变化规律,以便为鄱阳湖泊形态变化研究以及水、沙资源的合理开发利用提供有利的参考依据。运用水沙相关、上下游泥沙相关法对部分缺测泥沙进行插补,获得1956~2011年的鄱阳湖入、出湖水沙系列数据。利用数理统计法、水沙双累积曲线法、Mann-Kendall法分析鄱阳湖入、出湖径流量与输沙量变化特征,并且探讨了水沙变化的原因。结果表明:(1)入湖径流量和输沙量的年内分配相一致,而长江水沙的倒灌影响了出湖径流量和输沙量的年内分配;(2)鄱阳湖入湖悬移质泥沙从90年代开始下降趋势加剧,而出湖悬移质泥沙1956~2000年整体呈下降趋势,2001~2011年呈现上升趋势;(3)鄱阳湖入、出湖年输沙量出现明显的突变点分别在1998年左右、2003年左右;(4)径流量总体处于相对稳定状态,但近10a来径流量略下降,主要受到降水量变少的影响,入湖输沙量变化受"五河"流域的水库蓄水拦沙、流域水土保持工作加强、人类河床采砂等因素影响;而出湖河段河床深度下切、江湖水面比降加大、人类在鄱阳湖采砂扰动河(湖)床均加大了近10a来出湖悬移质泥沙的输出。     

Evaluation of potential genotoxicity of five food dyes using the somatic mutation and recombination test

In this study, different concentrations of five food dyes (amaranth, patent blue, carminic acid, indigotine and erythrosine) have been evaluated for genotoxicity in the Somatic Mutation and Recombination Test (SMART) of Drosophila melanogaster. Standard cross was used in the experiment. Larvae including two linked recessive wing hair mutations were chronically fed at different concentrations of the test compounds in standard Drosophila Instant Medium. Feeding ended with pupation of the surviving larvae. Wings of the emerging adult flies were scored for the presence of spots of mutant cells which can result from either somatic mutation or somatic recombination. For the evaluation of genotoxic effects, the frequencies of spots per wing in the treated series were compared to the control group, which was distilled water. The present study shows that carminic acid and indigotine demonstrated negative results while erythrosine demonstrated inconclusive results. In addition 25 mg mL⁻¹ concentration of patent blue and 12.5, 25 and 50 mg mL⁻¹ concentrations of amaranth demonstrated positive results in the SMART.     

流变-突变论在预防硫化矿自燃中的应用研究

利用安全流变-突变理论系统,分析了硫化矿石氧化自热到自燃整个过程的特征规律,确定了硫化矿堆自燃流变-突变的各个阶段,并提出了其流变-突变模型。根据模型提出了预防硫化矿堆自燃的安全管理及安全技术措施,例如:加强安全教育,完善规章制度等,建立完善的硫化矿石自燃火灾事故的应急预案机制等完善安全管理;合理设计通风系统,进行采场温度实时监测,喷洒阻化剂来抑制矿石的自热等主要安全技术措施。控制硫化矿堆自燃的关键在于将其控制在安全流变阶段,加强安全管理和采用安全技术,防止突变的发生,这对于硫化矿山的安全生产具有重大的指导意义。     

Allele frequencies and molecular diagnosis in haemophilia A and B patients from russia and from some Asian Republics of the former U.S.S.R.

RFLP analysis of some intra- and extra-genic polymorphic sites of Factor VIII (FVIII) and Factor IX (FIX) genes with relevant DNA probes or by polymerase chain reaction (PCR) was carried out in Slavic populations from the European part of Russia and also in the native ethnic groups of Uzbekistan and Kazahstan. The allele frequencies for the HindIII (intron 19) and XbaI (intron 22) polymorphic sites (PSs) in the FVIII gene were very similar in the two populations studied, but different for the intron 13 (CA)n repeat. Significant variations in the TaqI (intron d) and DdeI (intron a) polymorphisms of the FIX gene were evident between the Russian and Asian populations. Two unusual alleles (4·35 and 4·2 kb) for the extragenic PS St14/TaqI were registered in Slavs and one new allele (380 bp) for the DdeI polymorphic site of FIX was discovered in both Asian populations. Altogether, 210 haemophilia A (HA) and 24 haemophilia B (HB) families were subjected to molecular studies. So far, 160 HA and 12 HB families have been found to be informative for DNA analysis. Carrier status was ascertained in 42 HA and 6 HB female relatives, and rejected in 52 and 10 of them, respectively. The origin of some HA and HB mutations was traced with relevant polymorphic markers in several at-risk families. Prenatal diagnosis was accomplished in 28 HA and three HB families, resulting in the identification of 20 affected male fetuses.     

The synergistic interaction of Nickel(II) with dna damaging agents

In this report we present several examples in which nickel(II) in combination with DNA damaging agents caused an enhanced or synergistic biological response using several different endpoints. These examples of Ni(II) toxicity represent several approaches designed to understand the genotoxicity of Ni(II) as well as several other metal ions. They are discussed in this report as a partial basis for our hypothesis that Ni(II) may alter the cellular processing of DNA damage at some common point in the pathway for DNA repair of several different agents. In cultured Chinese hamster cells DNA damage by Ni(II) ions was not readily demonstrated by the method of alkaline elution, but pretreatment of cells with Ni(II) before X‐irradiation produced an enhanced amount of strand breaks compared to the amount produced by X‐rays alone. A synergistic enhancement of cell transformation of Syrian hamster embryo cells was observed for combined treatments of Ni(II) and benzo(a)pyrene. The nickel enhancement of mutagenesis by ultraviolet light was demonstrated for the bacterial gene gpt stably integrated into the Chinese hamster V79 genome.     

Research progress on CRISPR/Cas9-mediated genome editing technique in plants北大核心CSCD

Many physical, chemical, and biotic stresses always threaten plants during their growth and development. Using the genome editing technique is a good strategy to improve plant characteristics. CRISPR/Cas (clustered regulatory interspaced short palindromic repeats / CRISPR-associated protein), the adaptive and heritable immune system of prokaryotes, is a genome editing technique newly developed during recent years. At present, the type II CRISPR / Cas9 system is most widely used. Many plants, such as Arabidopsis thaliana, tobacco, soybean, tomato, potato, rice, wheat, maize, sorghum, petunia, banana, sweet orange, apple, poplar, and Marchantia polymorpha, have been edited successfully for stress tolerance, delay of fruit ripening, herbicide resistance, and disease resistance, etc. This system is achieved by the formation of nuclease Cas9 and crRNA: tracrRNA (CRISPR-derived RNA:trans-activating RNA) complex. Further studies about the effect of the promoters of Cas9 and gRNA and the number of gRNA targets on gene editing efficiency have been discussed. © 2018 Science Press. All rights reserved.     

群体感应信号分子存在下磺胺对大肠杆菌生长、突变及R388质粒接合转移的影响

抗生素的滥用使细菌耐药性问题日益突出,给许多疾病的预防与控制增加了难度。基因突变和质粒接合转移是细菌获得抗生素抗性基因的主要方式,许多研究围绕抗性基因来展开,但是关于群体感应对于抗性基因产生和传播的影响鲜有报道。本文以大肠杆菌(Escherichia coli)为模式生物,群体感应信号分子N-(β-酮己酰)-L-高丝氨酸内酯(3-oxo-C6-HSL,C6)和3种磺胺类抗生素(磺胺嘧啶、磺胺甲恶唑、磺胺氯哒嗪)为研究对象,测定了其对大肠杆菌生长效应、突变效应及接合转移效应的影响。结果表明:C6不影响磺胺对大肠杆菌的生长抑制率,但能够削弱磺胺对大肠杆菌突变的促进作用,并且能增强磺胺对大肠杆菌R388质粒接合转移的抑制作用。本文为从群体感应角度研究大肠杆菌耐药性的产生与传播提供新思路。