An Adaptive‐Management Framework for Optimal Control of Hiking Near Golden Eagle Nests in Denali National Park

Abstract: Unintended effects of recreational activities in protected areas are of growing concern. We used an adaptive‐management framework to develop guidelines for optimally managing hiking activities to maintain desired levels of territory occupancy and reproductive success of Golden Eagles (Aquila chrysaetos) in Denali National Park (Alaska, U.S.A.). The management decision was to restrict human access (hikers) to particular nesting territories to reduce disturbance. The management objective was to minimize restrictions on hikers while maintaining reproductive performance of eagles above some specified level. We based our decision analysis on predictive models of site occupancy of eagles developed using a combination of expert opinion and data collected from 93 eagle territories over 20 years. The best predictive model showed that restricting human access to eagle territories had little effect on occupancy dynamics. However, when considering important sources of uncertainty in the models, including environmental stochasticity, imperfect detection of hares on which eagles prey, and model uncertainty, restricting access of territories to hikers improved eagle reproduction substantially. An adaptive management framework such as ours may help reduce uncertainty of the effects of hiking activities on Golden Eagles.     

In vitro response of human ovarian cancer cells to dietary bioflavonoid isoquercitrin

Abstract

Isoquercitrin is a dietary bioflavonoid used as a food supplement. We studied the mechanism underlying its effect in human ovarian cancer cells using OVCAR-3 cell line. Viability, survival, apoptosis, release of human transforming growth factor-β1 (TGF-β1) and TGF-β1 receptor, and intracellular reactive oxygen species (ROS) generation by OVCAR-3 cells were examined after isoquercitrin treatment at concentrations 5, 10, 25, 50, and 100?μg mL^?1. AlamarBlue assay revealed that isoquercitrin did not cause any significant change (P?>?0.05) in cell viability as compared to control. Apoptotic assay using flow cytometry did not find any significant change (P?>?0.05) in the proportion of live, dead and apoptotic cells as compared to control. ELISA also showed that the release of human TGF-β1 and TGF-β1 receptor were not significantly (P?>?0.05) affected by isoquercitrin as compared to control. Chemiluminescence assay demonstrated that lower concentrations (5, 10, and 25?μg mL^?1) were able to exhibit beneficial effects by inhibiting the generation of intracellular ROS. In contrast, elevated concentrations of 50 and 100?μg mL^?1 led to oxidative stress (P?相似文献   

Intestinal absorption of the acetamiprid neonicotinoid by Caco-2 cells: Transepithelial transport,cellular uptake and efflux

The human intestinal absorption of acetamiprid (AAP) using the Caco-2 cell line reveals that AAP flux was active in a bidirectional mode with an apparent permeability coefficient of 26^.10^?6 cm·s^?1 at 37°C. Apical uptake was concentration-dependent and unsaturated for AAP concentrations up to 200 μ M. AAP cell preloading demonstrated the involvement of active transport mechanisms. Arrhenius plot analysis revealed an unusual profile with two apparent activation energies suggesting two transport processes. Uptake Vi studies indicated the involvement of a sodium-dependent transporter, the presence of a common transporter of AAP and nicotine and the involvement of Ti-sensitive ATP-dependent efflux transporters. Apical efflux investigations showed the involvement of inward active transporter(s). Whereas vincristine had no effect on intracellular accumulation, taxol and daunorubicin treatments unexpectedly led to 10% and 23% reductions respectively, suggesting that the latter shared a common inward transporter with AAP. All these results suggest full and express AAP absorption in vivo with transport involving both inward and outward, passive and active mechanisms. Thus, AAP or its metabolites could be representative of a risk for human health after its ingestion in food.     

The Frequency of Micronuclei among Newborns from Kragujevac, Central Serbia, after NATO Bombing in the Spring of 1999

Kragujevac, as an important industrial and economic center of the region, is now placed on the top of the list of environmentally endangered cities in our country. The aim of this biomonitoring study was to evaluate cytogenetic damage in a sample of newborns from Kragujevac after contamination of the environment entailed by the intensive bombing of the industrial zone of this city in the spring of 1999. The frequency of micronuclei (MN) in peripheral blood lymphocytes in a total of 61 phenotypically healthy newborns was analyzed before and after the NATO bombing. Analysis of micronuclei has been performed using the cytokinesis-block technique (CB MN test). Average MN frequency in lymphocytes of newborns before the bombing (N = 25) was 5.77 ± 0.85/1000 analyzed cells. After the bombing (N = 36), the average frequency of MN increased by a factor of 1.4 (8.11 ± 0.85), compared to the control frequency before the bombing. Statistical difference (p < 0.05) was established by Student's t-test. Our data suggest that such changes in genetic material were a direct consequence of contamination of the living environment.     

考虑人为因素的可靠性评估

统计数据表明,系统的可靠运行不可忽略人为误操作造成的故障,该故障发生率有时甚至超过设备本身导致的系统故障发生率。将人为因素与设备自身因素造成的系统故障进行对比,分析人为因素造成系统故障后的特点,可得出人为因素的可靠性参数。根据解析法和模拟法的相关理论计算考虑人为因素的可靠性指标,可以看出考虑人为因素的计算结果更加符合实际运行特点,便于相关人员采取对策,保障系统的安全运行。     

Prenatal diagnosis of 5p deletion syndrome following abnormally low maternal serum human chorionic gonadotrophin

Prenatal diagnosis of 5p deletion syndrome, or cri du chat, following an abnormally low measurement of a screening of serum human chorionic gonadotrophin (hCG), is reported. Karyotyping following amniocentesis revealed a terminal deletion in the short arm of one chromosome 5. The pregnancy was electively terminated. 5p deletion syndrome has been described with abnormally high hCG levels and normal hCG levels. This is the first report of its association with abnormally low levels. The association between chromosomal abnormalities and hCG is discussed. Copyright © 2003 John Wiley & Sons, Ltd.     

基于层次分析法的Pedersen人因失误模型研究

科技的发展,使机器设备可靠性有了显著提高,工作环境也得到了改善,但由于人的复杂性,人因失误成为主要的致因因素,如何预防和控制人的失误就成为一个非常重要的课题.运用层次分析法的基本原理,结合Pedersen人因失误模型,构建了导致人因失误的层次分析模型,计算结果显示,人在某种状态下承受负荷的能力大小是影响其失误的主要原因.通过实例,证明了计算结果的可靠性,并提出了减少人因失误的建议.     

基于人地耦合系统的国土空间重塑

地理系统主要研究地球表层人与环境相互作用的机理,强调人地关系、自然和人文要素的综合,即人地耦合系统。地理系统在国土空间中的应用主要由地理决策、地理模拟、地理工程所组成,并且相互作用构成了地理治理。国土空间是自然与人类所共同实践的物质载体,人类被自然所塑造的程度并不亚于自身塑造自然的程度,以人地耦合系统为核心的地理系统理论成为国土空间规划与整治修复的理论基础。基于人地耦合系统的国土空间重塑最终以地理治理的形式反映在人类作用于自然环境的各种活动中。经过改革开放40年的发展,中国国土空间已基本形成了较为稳定的格局,形成了经济区、贫困区、小城镇为主的三种形态空间。中国的城镇化发展与发达国家有所不同,是城市化、城镇化、乡村化“三化耦合”并存的状态。必须重视泛第三极、环中国南海、东北亚—北极等以“一带一路”全球空间为基础的战略区域研究。基于人地耦合系统理论与国土空间价值均衡理论,国土空间重塑的基础科学问题是人地耦合系统演化机理与驱动机制。国土空间规划可以划分为三种类型：发展型规划、控制型规划和修复型规划。国土空间的保护与治理包括以土地利用为核心的国土空间全域整治,以生态文明为核心的国土空间系统修复,以社会和谐为核心的国土空间综合治理。人地耦合系统最终的发展目标是形成人类与自然相互作用的命运共同体。     

Second-trimester maternal serum alpha-fetoprotein (MSAFP) iselevated in women with adverse pregnancy outcome associated with inherited thrombophilias

Obstetric complications, such as severe pre-eclampsia, fetal growth restriction, abruptio placentae, or stillbirth are associated with abnormally elevated second-trimester maternal serum alpha-fetoprotein (MSAFP) and β subunit of human chorionic gonadotrophin (βhCG). This has been attributed to placental abnormalities. Women with thrombophilias have been shown to have abnormalities of the placenta resulting in adverse pregnancy outcome in these patients. The purpose of the present study was to evaluate whether women with pregnancy complications and inherited thrombophilias have abnormally elevated second-trimester MSAFP or βhCG. Sixty-two women with pregnancy complications were tested for inherited thrombophilias several months after delivery. The thrombophilia group included 29 women with pregnancy complications and an inherited thrombophilia and the control group included 33 other patients without thrombophilia. Patients in the thrombophilia group had a higher median MoM MSAFP compared to the controls (1.337 vs 1.086, p=0.0516). The incidence of abnormally elevated MSAFP (>2.5 MoM) was also significantly higher in the thrombophilia group compared to controls (21% vs 3%, p=0.04). Neither the median MoM βhCG nor the incidence of abnormally elevated βhCG were significantly different between the groups. We conclude that second trimester MSAFP, but not βhCG, is abnormally elevated in patients with thrombophilia and obstetric complications. Copyright © 2001 John Wiley & Sons, Ltd.     

21世纪住区建设

面对世界发展趋势和中国建设高潮期,21世纪住宅建设需体现可持续发展,引入信息技术和新材料等高新技术,适应居住生活的发展。本文概述了当前世界发展趋势和国家住宅建设目标,住宅建设面临的科技问题,着重论述21世纪住区建设的规划设计原则。