An investigation of methods for enriching trophoblast from maternal blood

Trophoblast deportation is known to occur in normal human pregnancy, but it is not yet clear whether these cells routinely enter the maternal peripheral circulation and are available as a source of fetal DNA for non-invasive prenatal diagnosis of genetic disorders. To resolve this issue requires an efficient method of enriching trophoblast from maternal blood combined with a means to confirm its identity. Five different techniques were tested on ten retroplacental blood samples to determine the most sensitive and operator-efficient method. Lysis of red cells alone gave the best recovery of trophoblast but had to be discounted, together with Ficoll density gradient centrifugation, due to the very low purity and the excessive time required. Fluorescence-activated cell sorting (FACS) of pre-enriched trophoblast resulted in the lowest recovery rate (8 per cent) despite a 3250-fold enrichment and a very high purity. Immunomagnetic beads (Dynabeads) coated with anti-CD 16 antibody proved to be the best method for the subsequent immunocytochemical characterization of deported trophoblast. However, IO beads coated with anti-CD45 antibody may be more useful for isolating trophoblast for prenatal diagnosis due to the high purity, enrichment (32-fold), and recovery rate (78 per cent) obtained with this method.     

华南玄武岩红土化过程中微量元素地球化学

广东、海南的玄武岩．在强烈的红土化过程中．元素在红土剖面中发生迁移和残留、贫化和富集。根据红土化过程中元素的地球化学性状，将元素划分为活泼元素和不活泼元素两大类。并首次采用了元素它集因子这个概念来探讨红土化过程中微量元素的地球化学行为。最后讨论了红土化过程小金的表生富集作用。     

Prenatal investigation of a 45,X/46,X,r(?) karyotype in amniocytes using fluorescence in situ hybridization with an X-centromeric probe

The karyotype of cultured amniotic fluid cells obtained on the indication of advanced maternal age was shown to be a mosaic 45,X/46,X,r(?). The small size and banding pattern made it difficult to determine whether the ring was derived from and X or a Y chromosome, or even from an autosome. By using an X-centromeric probe and fluorescence in situ hybridization (FISH), we demonstrated the ring to have an X centromere. Thus, a more complete genetic counselling was possible. This confirms the usefulness of FISH in identifying and characterizing this and other chromosome rearrangements in prenatal diagnosis.     

在污染条件下生物的生态效应

主要就污染物对动物、植物及人体健康的危害，以及在污染条件下生物的适应与进化作一综述。     

浅谈ISO14000环境管理体系标准对推进清洁生产的积极影响

针对ISO14000环境管理体系的特点论述了其对推进清洁生产的积极作用。     

生态技术创新政府启动模式的机理与启示

生态技术创新是经济可持续发展的必要条件。由于环境资源的公共性，时滞性等特点，政府在生态技术创新过程中发挥重要的作用，是企业生态技术创新的主要推动力。政府启动模式的本 于政府借助法律或经济手段促使企业部分边际外部费用内部化。     

正己烷萃取—此外分光光度法测定矿井水中微量油

在进行矿井水中油类分析时，用正己烷作为萃取剂，以工作曲线代替标准曲线，从而使油平均回收率增加到96％以上。并在正己烷用量、水样预处理等测试参数选择上做了条件试验。     

热液中金的沉淀机理研究综述

金的沉淀机理是了解热液金矿床成矿机制的关键。本文总结了金从热液中沉淀机理的最新研究成果，介绍了各种主要含金配合物的溶解度拨制机制，阐述了矿物表面吸附／还原以及电化学富集等矿物表面作用对金沉淀行为的制约。     

唤起“求知欲”，治疗“厌学症”

本文分析了求知欲的由来及消退原因，“厌学症”的形成及治疗方案，试图唤起学生的求知欲，治疗他们的“厌学症”。     

沪杭高速公路嘉善段道路声屏障的设计及降噪效果

在详细调查沪杭高速公路喜善张家浜中桥路段（试验路段）未建道路声屏障噪声情况的基础上，介绍了试验路段工程概况及道路声屏障的设计，并分析了声屏障的现场降噪效果，试验路段声屏障的声学指标达到预期目标，具有良好的环境效益。