山区农村居民点时空格局演变特征及蚁群仿真优化——以重庆澄江镇为例

山地农村居民点系统因受自然因素及人类活动等多重扰动而处于深刻转型中,探索山区农村居民点时空格局特征及其优化策略,对于优化山区国土空间格局、推进山区国土空间综合治理与乡村振兴具有重要意义。基于构建理论框架,集成运用景观格局指数法、栅格权重叠加评价法、改进蚁群算法（ACO）等,以重庆澄江镇为例,探索山区农村居民点时空格局演变特征及优化过程,提出优化调控策略。结果表明：（1）研究区农村居民点空间格局演变特征呈现集中、靠拢的演化发展趋势,总体呈现匀质、线状、向心、院落四种分布形态;同时高程、坡度、水域、城镇、道路、基础设施等自然—经济社会因素均对山区农村居民点时空格局演化产生重要影响。（2）改进蚁群算法优化后,研究区农村居民点斑块面积（CA）减少10.07%,居民点斑块数量（NP）和密度（PD）分别减少83.12%和81.16%,平均斑块面积（MPS）增加了4.3倍,平均最近邻体距离（MENN）增加了42.82%。优化后69.1%的农村居民点斑块都集中在适宜区和较适宜区内,且重点向交通便利、公共设施完善、生产便利和中心村庄集中。优化后的农村居民点空间格局更加紧凑有序,土地集约节约效率更高。（3）基于仿真优化结果,综合考虑村庄振兴“产业—人口—土地”互动耦合机制,提出城郊融合、产业集聚、景村融合、搬迁撤并四类农村居民点格局优化方案,助推山区乡村振兴。（4）优化结果同时表明改进的蚁群算法非常适用于复杂环境条件下的较小尺度要素空间布局优化,能够为山区国土空间规划编制提供技术支撑。     

遗产区域:一种大尺度文化景观保护的新方法

遗产区域是美国国内针对大尺度文化景观保护的一种较新的方法。该方法重视人与土地之间的互动关系。强调通过对地方历史文化、自然和游憩资源的综合保护与利用。实现遗产保护、经济发展、重建区域身份、提供游憩机会等多重目标。从美国历史保护运动的发展背景出发。回顾了遗产区域的发展历程，从定义、特征与分类三个方面评述了其概念内涵，并对遗产区域保护中的几个关键问题进行了讨论。最后针对遗产区域保护方法在中国的发展与应用提出了相关建议。     

唐家河保护区主要植被类型景观格局的梯度分析

在遥感图像基础上，利用GIS技术从景观指数方面定量分析了唐家河自然保护区主要植被类型在东西、南北和西北至东南3个方向上的梯度变化。结果表明：次生落叶阔叶林、常绿落叶阔叶混交林、针阔叶混交林与针叶林的梯度变化明显，并且在各方向上具有不同的变化特征。其中，常绿落叶阔叶混交林从西北至东南方向的梯度变化最为复杂， 斑块数量与面积分别呈“升—降—升—降”与“升—降—升”的波动变化趋势，而边界密度与平均最近距离呈先升后降的变化趋势，两端破碎度高但连接性好，中部相反。针叶林从北至南的梯度变化最为简单，斑块面积减少，破碎度与复杂度降低，南北两端分布较多，中部较少。唐家河自然保护区植被景观格局在不同方向上的梯度变化研究为地震后该地区的植被保护与管理提供重要指导意义。     

Integration of technologies and alternative sources of water and energy to promote the sustainability of urban landscapes

Recent research has highlighted the positive role of green areas in urban environments, providing numerous social, environmental and economic services, such as mitigation of the urban heat island effect, storm attenuation, increased water infiltration into the soil, reduction of noise and air pollution, among others. However, the maintenance of green areas may result in high consumption of water, reaching 50% of the total consumption in some municipalities, and energy, becoming a reason of concern. The present study aimed to integrate techniques and technologies that promote the automatic and efficient irrigation of urban landscapes, using alternative sources of energy and water, toward its sustainability. The conceptual unit was able to reduce in 64% the water consumed in irrigation. Rainwater met 69% of the demand and the photovoltaic system supplied all the energy required. The economic feasibility analysis showed that the conceptual unit is financially unfeasible, under the conditions of this study. However, with some interventions for reusing the surplus energy and water, and considering the higher fees charged by other cities, the investment became attractive. In this new scenario, the internal rate of return (15 years) was 27.3% and the discounted payback period was 4.9 years.     

基于复合生态系统理论的长沙湘江滨水区景观资源评价与优化

近年来，随着我国经济的飞速发展、社会经济的转型，以及人们环境意识的提高，滨水区开发已成为我国城市开发建设的热点。城市滨水区景观规划设计研究对于合理、充分利用城市滨水区景观资源，塑造特色鲜明的城市形象，提高城市生态环境质量，促进城市滨水区开发有着重要意义。从复合生态系统理论的角度，在湘江滨水区景观资源调查、景观资源分类的基础上，运用灰色聚类法对湘江滨水区景观资源进行定量评价，提出景观资源优化思路，探讨滨水区景观规划设计的途径。认为现代城市大规模滨水区更新改造和开发大背景下的城市滨水区景观规划设计，应尊重城市地域性特点、文化内涵和风土人情,并与传统的滨水活动有机结合，保护和突出城市历史格局和风貌特色。     

碳源及反硝化芽孢杆菌强化低C/N比景观水体的生物脱氮

实验探讨了添加碳源及投加反硝化细菌对低碳氮比景观水体生物脱氮的影响。结果表明,有机碳源及B.subtilis FS05均能显著促进实验水体的生物脱氮作用,实验水体在28℃静置72 h后,乙醇添加组的TN、氨氮、硝酸盐及亚硝酸盐的去除率分别达到了62.7%、67.0%、69.8%和29.4%,而同样条件下,B.subtilis FS05投加组的去除率分别达到了66.9%、73.4%、66.0%和82.2%。从水质变化趋势可以看出,投加B.subtilis FS05能在更短时间内完成生物脱氮过程,其中,硝酸盐和亚硝酸盐去除速率最快,分别仅需要18 h和12 h。     

雅砻江流域湿地景观格局变化分析

应用ArcGIS软件，对雅砻江流域湿地1986和2000年遥感数据进行解译、矢量化处理，用Apack软件进行湿地景观格局指数计算，研究了近15 a雅砻江流域景观空间格局特征及其变化。结果表明：（1）近15 a雅砻江流域湿地景观面积减少，减少量为49404 hm2，占2000年湿地总面积的0506%；（2）两个时期内湿地斑块密度保持不变，湿地景观的聚集度都很高，均在099以上，湿地景观集中分布，破碎化水平低；（3）雅砻江流域湿地景观的多样性指数水平和均匀度水平较低，而且两指数在两个个时期内没有发生太大的变化，且各类湿地景观比例差异相对较大，沼泽湿地景观控制的程度较高；（4）雅砻江流域的5类湿地景观分布质心基本上未发生变化，但总体格局分布质心向南偏移001°，再向西偏移023°，整体上向西南方向偏移了2532 km     

Assessing Landscape Condition Relative to Water Resources in the Western United States: A Strategic Approach

The Environmental Monitoring and Assessment Program (EMAP) is proposing an ambitious agenda to assess the status of streams and estuaries in a 12-State area of the western United States by the end of 2003. Additionally, EMAP is proposing to access landscape conditions as they relate to stream and estuary conditions across the west. The goal of this landscape project is to develop a landscape model that can be used to identify the relative risks of streams and estuaries to potential declines due to watershed-scale, landscape conditions across the west. To do so, requires an understanding of quantitative relationships between landscape composition and pattern metrics and parameters of stream and estuary conditions. This paper describes a strategic approach for evaluating the degree to which landscape composition and pattern influence stream and estuary condition, and the development and implementation of a spatially-distributed, landscape analysis approach.     

2,4-D Mineralization in soil profiles of a cultivated hummocky landscape in Manitoba,Canada

The objective of this study was to quantify 2,4-D (2,4-dichlorophenoxyacetic acid) mineralization in soil profiles characteristic of hummocky, calcareous-soil landscapes in western Canada. Twenty-five soil cores (8 cm inner diameter, 50 to 125 cm length) were collected along a 360 m transect running west to east in an agricultural field and then segmented by soil-landscape position (upper slopes, mid slopes, lower slopes and depressions) and soil horizon (A, B, and C horizons). In the A horizon, 2,4-D mineralization commenced instantaneously and the mineralization rate followed first-order kinetics. In both the B and C horizons, 2,4-D mineralization only commenced after a lag period of typically 5 to 7 days and the mineralization rate was biphasic. In the A horizon, 2,4-D mineralization parameters including the first-order mineralization rate constant (k ₁), the growth-linked mineralization rate constant (k ₂) and total 2,4-D mineralization at the end of the experiment at 56 days, were most strongly correlated to parameters describing 2,4-D sorption by soil, but were also adequately correlated to soil organic carbon content, soil pH, and carbonate content. In both B and C horizons, there was no significant correlation between 2,4-D mineralization and 2,4-D sorption parameters, and the correlation between soil properties and 2,4-D mineralization parameters was very poor. The k ₁ significantly decreased in sequence of A horizon (0.113% day^?1) > B horizon (0.024% day^?1) = C horizon (0.026% day^?1) and in each soil horizon was greater than k ₂. Total 2,4-D mineralization at 56 days also significantly decreased in sequence of A horizon (42%) > B horizon (31%) = C horizon (27%). In the A horizon, slope position had little influence on k ₁ or k ₂, except that k ₁ was significantly greater in upper slopes (0.170% day^?1) than in lower slopes (0.080% day^?1). Neither k ₁ nor k ₂ was significantly influenced by slope position in the B or C horizons. Total 2,4-D mineralization at 56 days was not influenced by slope positions in any horizon. Our results suggest that, when predicting 2,4-D transport at the field scale, pesticide fate models should consider the strong differences in 2,4-D mineralization between surface and subsurface horizons. This suggests that 2,4-D mineralization is best predicted using a model that has the ability to describe a range of non-linear mineralization curves. We also conclude that the horizontal variations in 2,4-D mineralization at the field scale will be difficult to consider in predictions of 2,4-D transport at the field scale because, within each horizon, 2,4-D mineralization was highly variable across the twenty-five soil cores, and this variability was poorly correlated to soil properties or soil-landscape position.     

Modulation of steroidogenesis by coastal waters and sewage effluents of Hong Kong, China, using the H295R assay

BACKGROUND, AIM, AND SCOPE: The presence of a variety of pollutants in the aquatic environment that can potentially interfere with the production of sex steroid hormones in wildlife and humans has been of increasing concern. The aim of the present study was to investigate the effects of extracts from Hong Kong marine waters, and influents and effluents from wastewater treatment plants on steroidogenesis using the H295R cell bioassay. After exposing H295R cells to extracts of water, the expression of four steroidogenic genes and the production of three steroid hormones were measured. MATERIALS AND METHODS: Water samples were collected during the summer of 2005 from 24 coastal marine areas and from the influents and effluents of two major waste water treatment plants (WWTPs) in Hong Kong, China. Samples were extracted by solid phase extraction (SPE). H295R cells were exposed for 48 h to dilutions of these extracts. Modulations of the expression of the steroidogenic genes CYP19, CYP17, 3betaHSD2, and CYP11beta2 were determined by measuring mRNA concentrations by real-time polymerase chain reaction (Q-RT-PCR). Production of the hormones progesterone (P), estradiol (E2), and testosterone (T) was quantified using enzyme linked immunosorbent assays (ELISA). RESULTS: Extracts from samples collected in two fish culture areas inhibited growth and proliferation of H295R cells at concentrations greater or equal to 10(5) L equivalents. The cells were exposed to the equivalent concentration of active substances in 10,000 L of water. Thus, to observe the same level of effect as observed in vitro on aquatic organisms would require a bioaccumulation factor of this same magnitude. None of the other 22 marine samples affected growth of the cells at any dilution tested. Twelve of the marine water samples completely inhibited the expression of CYP19 without affecting E2 production; inhibition of CYP17 expression was observed only in one of the samples while expression of CYP11beta2 was induced as much as five- and ninefold after exposure of cells to extracts from two locations. The expression of the progesterone gene 3betaHSD2 was not affected by any of the samples; only one sample induced approximately fourfold the production of E2. Although more than twofold inductions were observed for P and T production, none of these values were statistically significant to conclude effects on the production of these two hormones. While influents from WWTPs did not affect gene expression, an approximately 30% inhibition in the production of E2 and a 40% increase in P occurred for the exposure with influents from the Sha Tin and Stonecutters WWTPs, respectively. Effluents from WWTPs did not affect the production of any of the studied hormones, but a decrement in the expression of the aldosterone gene CYP11beta2 was observed for the Sha Tin WWTP exposure. No direct correlation could be established between gene expression and hormone production. DISCUSSION: Observed cytotoxicity in the two samples from fish culture areas suggest the presence of toxic compounds; chemical analysis is required for their full identification. Although effluents from WWTPs did not affect hormone production, other types of endocrine activity such as receptor-mediated effects cannot be ruled out. Interactions due to the complexity of the samples and alternative steroidogenic pathways might explain the lack of correlation between gene expression and hormone production results. CONCLUSIONS: Changes observed in gene expression and hormone production suggest the presence in Hong Kong coastal waters of pollutants with endocrine disruption potential and others of significant toxic effects. The aromatase and aldosterone genes seem to be the most affected by the exposures, while E2 and P are the hormones with more significant changes observed. Results also suggest effectiveness in the removing of compounds with endocrine activity by the WWTPs studied, as effluent samples did not significantly affect hormone production. The H295R cell showed to be a valuable toll in the battery required for the analysis of endocrine disrupting activities of complex environmental samples. RECOMMENDATIONS AND PERSPECTIVES: Due to the intrinsic complexity of environmental samples, a combination of analytical tools is required to realistically assess environmental conditions, especially in aquatic systems. In the evaluation of endocrine disrupting activities, the H295R cell bioassay should be used in combination with other genomic, biological, chemical, and hydrological tests to establish viable modes for endocrine disruption and identify compounds responsible for the observed effects.