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101.
通过砂培试验,研究了铅(Pb)胁迫下不同品种玉米根、茎叶中Pb的化学形态变化,进一步探讨玉米耐Pb机制.结果表明,本试验中对Pb胁迫耐性最强的玉米品种为郑单958和隆平206,耐性最差的为联创5号.不同浓度Pb胁迫下玉米根、茎叶中Pb主要以无毒的醋酸提取态和盐酸提取态为主,比例高达60%~87%,这一比例根部略高于茎叶;而活性较强的乙醇提取态和水提取态Pb含量两者合计所占比例为6%~20%.100 mg·L-1Pb胁迫下隆平206茎叶中Pb的乙醇和水提取态合计值最低(0.52 mg·kg-1),其次是郑单958(0.93 mg·kg-1),最高的为联创5号(2.78 mg·kg-1);800 mg·L-1Pb胁迫下,郑单958茎叶中Pb的乙醇提取态和水提取态合计值最低,为2.41 mg·kg-1,因此郑单958耐Pb,可能与体内有毒形态Pb向无毒形态Pb的转换有关. 相似文献
102.
Ahmed S.A. El-shakh Kaleem Ullah Kakar Xiao Wang Abdulwareth A. Almoneafy Mohammad Raza Ojaghian Bin Li 《毒物与环境化学》2015,97(6):766-785
This study was conducted to assess efficacy of biological control against bacterial leaf blight (BLB) of rice produced by Xanthomonas oryzae pv. oryzae. Five endophytic strains (A1, A2, A3, A13 and A15) and two rhizospherial Bacilli (D29 and H8) were tested for their antagonistic activities against BLB in vitro and in vivo. All seven strains showed high potential of antagonistic activity against X. oryzae pv. oryzae and three phytopathogenic fungi in vitro. Test of 16SrRNA gene sequence were assigned isolates A1, A3 and A13 as Bacillus amyloliquefaciens while isolates A2 and A15 as B. methylotrophicus and B. subtilis, respectively. In greenhouse, four strains of displayed 50.29%–57.86% inhibition rate against the pathogen and significantly increased plant fresh weight from 50.03% to 73.11% and dry weight from 64.11% to 86.65% in treated rice plants. In addition, these strains demonstrated strong capability to produce indole-3-acetic acid, siderophores, solubilizing phosphate and also colonize roots. Real-time quantitative polymerase chain reaction revealed that expression of defense-related genes including OsAOS2, OsJMT1, OsNPR1 and OsPR1b were significantly up-regulated in leaves of D29-exposed rice plants, suggesting that treatment of rice with D29 suppressed BLB through systemic activation of the plant defense system. Therefore, data suggest that Bacillus isolates A13, A15, D29 and H8 support effective antagonistic activity against BLB under greenhouse conditions in addition to their potential to promote growth of rice plants. 相似文献
103.
丛枝菌根真菌(AMF)对铯胁迫宿根高粱生长及根际土壤酶的影响 总被引:1,自引:0,他引:1
通过盆栽试验研究了接种透光球囊霉(Glomus diaphanum)、摩西球囊霉(Glomus mosseae)、地表球囊霉(Glomus versiforme)及幼套球囊霉(Glomus etunicatum)等4种丛枝菌根真菌(AMF)对铯污染下宿根高粱生长及根际土壤酶活性的影响.结果表明,与未接种对照相比,接种AMF处理均显著增加了宿根高粱的株高和根长,同时增强了过氧化氢酶、蔗糖酶活性及根系活力(P0.05),且以接种G.etunicatum增强效果最好,上述酶活性和根系活力分别相当于对照的1.11、2.25和4.04倍,而接种Glomus diaphanum最显著增强了酸性磷酸酶活性,使其提高了24.53 mg·g-1·d-1·FW(P0.05),宿根高粱对铯的耐性指数均高于对照,说明AMF在一定程度上能缓解铯污染胁迫对根际土壤酶的抑制作用,进而减轻核素铯对植物的毒害;接种AMF处理显著抑制了脲酶活性,表明脲酶可作为反应土壤核素铯污染的一个有效指标.同时也表明AMF有效地增强了宿根高粱对核素铯的耐性能力. 相似文献
104.
保护性耕作下小麦田土壤呼吸及碳平衡研究 总被引:12,自引:7,他引:5
为了研究小麦农田生态系统土壤碳排放与作物碳蓄积特征,采用LI6400-09在重庆北碚西南大学教学试验农场对平作(T)、垄作(R)、平作+覆盖(TS)、垄作+覆盖(RS)这4种处理下的西南紫色土丘陵区小麦/玉米/大豆套作体系中小麦生长季节的土壤呼吸及植株生长动态进行了观测.利用根系生物量外推法(root biomass regression,RBR)和根排除法(root exclusion,RE)这2种方法比较分析根系呼吸对土壤总呼吸的贡献,并估算小麦农田碳收支状况.结果表明,土壤呼吸介于0.62~2.91μmol·(m2·s)-1,平均值为1.71μmol·(m2·s)-1.T、R、TS、RS各处理日均土壤呼吸速率分别为1.29、1.59、1.99、1.96μmol·(m2·s)-1,表现为T相似文献
105.
苹果园土壤呼吸的变化及生物和非生物因素的影响 总被引:1,自引:1,他引:0
了解果园土壤呼吸变化及其影响因素,有利于深入理解退耕还果条件下黄土高原地区土壤碳源汇功能.在长武农田生态系统国家野外站,以盛产期果园(2000年建成)为对象,利用土壤碳通量测量系统(Li-COR,Lincoln,NE,USA)于2011、2012年监测了果树冠幅下距树干不同距离处土壤呼吸、土壤水分和温度变化,分析了土壤呼吸的时空变化及其影响因素.结果表明:①土壤呼吸速率随着距树干距离延长而降低.与2 m处相比,0.5 m处土壤累积呼吸量2011年提高20%,2012年提高31%;0.5 m和2 m处土壤呼吸的温度敏感性(Q10)2011年相应依次为1.79和1.56,2012年依次为1.79和1.38.②距树干2 m处温度和水分稍高于0.5 m处,但差异不显著(P>0.05).土壤呼吸与土壤温度均呈显著的指数关系,而与土壤水分的相关性不显著.温度变化可解释土壤呼吸的季节性变化,但并不能解释距离树干不同处的差异.③距离树干不同位置处的根系密度差异是影响果园土壤呼吸空间变化及其温度敏感性的重要生物因素;④冠幅下土壤呼吸的变异系数为23%~31%.估算果园土壤呼吸需考虑其距离树干的空间差异性. 相似文献
106.
流化床生物反应器培养青蒿毛状根生产青蒿素 总被引:3,自引:0,他引:3
利用自制的流化床生物反应器进行青蒿毛状根多层培养生产青蒿素.对毛状根在生物反应器生长过程的形态特征进行观察,并在合适的工艺条件下,经20d分批培养获得生物量干重21.3g/L,青蒿素产量349.8mg/L,并对培养过程中底物消耗的动力学进行了研究. 相似文献
107.
M.R. Savabi J.G. Arnold C.W. Richardson J. L. Krishna 《Journal of the American Water Resources Association》1989,25(4):855-865
ABSTRACT: With the increase in water demand in Texas, attention has turned to improving water yield by brush control on rangeland watersheds. Several hydrologic models have been developed for either farmland or rangeland. However, none of the models were specifically developed to assess the impact of brush control on rangeland water yield. Yet, modeling the impact of brush control on water yield needs to be considered if alternative techniques are to be compared. Two models, Ekalaka Rangeland Hydrology and Yield Model (ERHYM-II) and Simulator for Water Resources on Rural Basins (SWRRB) were selected. The Soil Conservation Service curve number (SCS-CN) method is used in both models to predict surface runoff from each rainfall event. The major differences between the ERHYM-II and SWRRB models are the evapotranspiration, soil water routing, and plant growth components. The models were evaluated on brush-dominated and chemically and mechanically brush-controlled range watersheds in Texas. Results indicated that both models were capable of simulating soil water and water yield from brush dominated and chemically brush-controlled range watersheds. The models were not able to predict water yield from the mechanically brush-controlled (root plowed) watershed with acceptable accuracy. The depressions that were caused by root plowing stored surface runoff and reduced water yield from the watershed. Information about the size of depressions was not available for further model evaluation. 相似文献
108.
109.
In order to improve and optimize phytoremediation of PAH we propose to focus on the rhizospheric processes controlling PAH
degradation. In this paper the effect of root exudates on PAH availability is studied. Model organic compounds (malic acid,
malonic acid and EDTA) representing root exudates have been tested for their effect on phenanthrene sorption on a reference
non polluted agricultural soil material. Phenanthrene adsorption isotherms were first obtained with batch experiments. Results
showed linear isotherms and phenanthrene sorption was enhanced as the concentration of organic compounds in the solution increased.
Column leaching experiments were then used to simulate the effect of root exudation following the soil pollution. Inlet solutions
containing the different organic acids used were flowed through the column containing the artificially polluted soil material.
Elution curves showed that the phenanthrene was less easily eluted when the solution injected contained the organic acids.
However, magnitude of the phenomena did not fit with adsorption constants obtained in batch experiments. Phenanthrene desorption
appeared limited by sequestration but organic acids seemed able to partially disturb the soil material structure to limit
the sequestration effect. 相似文献
110.
IntroductionOneofthemajordrawbacksofalleycroppingsystemsinsemiaridlowfertilityconditionsiscompetitionforavailablewaterandnutrients.Competitionistobeexpectedifthetreeandthecrophaveoverlappingactiverootsystemsinspaceandtime.Aknowledgeofthespatialdistr… 相似文献