全文获取类型
收费全文 | 326篇 |
免费 | 31篇 |
国内免费 | 104篇 |
专业分类
安全科学 | 121篇 |
废物处理 | 5篇 |
环保管理 | 28篇 |
综合类 | 220篇 |
基础理论 | 58篇 |
污染及防治 | 16篇 |
评价与监测 | 2篇 |
社会与环境 | 2篇 |
灾害及防治 | 9篇 |
出版年
2024年 | 1篇 |
2023年 | 8篇 |
2022年 | 8篇 |
2021年 | 17篇 |
2020年 | 8篇 |
2019年 | 7篇 |
2018年 | 9篇 |
2017年 | 12篇 |
2016年 | 19篇 |
2015年 | 16篇 |
2014年 | 11篇 |
2013年 | 16篇 |
2012年 | 25篇 |
2011年 | 32篇 |
2010年 | 29篇 |
2009年 | 25篇 |
2008年 | 29篇 |
2007年 | 36篇 |
2006年 | 24篇 |
2005年 | 16篇 |
2004年 | 14篇 |
2003年 | 12篇 |
2002年 | 12篇 |
2001年 | 8篇 |
2000年 | 6篇 |
1999年 | 5篇 |
1998年 | 3篇 |
1997年 | 2篇 |
1996年 | 3篇 |
1995年 | 12篇 |
1994年 | 10篇 |
1993年 | 6篇 |
1992年 | 11篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1989年 | 4篇 |
1988年 | 2篇 |
1986年 | 1篇 |
排序方式: 共有461条查询结果,搜索用时 156 毫秒
11.
Rapid detection of numerical aberrations of chromosomes 13, 18 and 21 in chorionic mesenchymal cells
We have devised and evaluated a rapid screening method for the detection of numerical aberrations of chromosomes13, 18 and 21 in chorionic villus cells. We used non-radioactive in situ hybridization (ISH) with three chromosome-specific probes on overnight-attached mesenchymal cells from chorionic villi. A blind study was performed of 47 karyotypically normal samples, one triploid sample, two samples trisomic for chromosome 21, and two samples from a fetus with putative mosaicism (46/47, +21). All samples were hybridized with the chromosome 18- and 21-specific probes. Thirty samples were additionally hybridized with the chromosome 13-specific probe. The test could be completed within 3-4 days of sampling. In samples disomic with respect to the probed chromosomes, an average of 2 per cent (range 0-9 per cent) had three hybridization signals. By contrast, in the samples trisomic for the probed chromosome(s), 57 per cent (chromosome 13), 51 per cent (chromosome 18), and an average of 74 per cent (55-86 per cent) (chromosome 21) of the nuclei exhibited three signals. In the putative mosaic samples, the number of nuclei with three chromosome 21-specific signals ranged from 41 to 69 per cent. We conclude that this technique rapidly and clearly distinguishes between normal and trisomic/triploid samples, and consequently may be of use in future prenatal diagnosis. 相似文献
12.
Thirty-six pregnancies in 25 families at risk of β-thalassaemia major received prenatal diagnosis. Chorionic villus sampling or amniocentesis was done in 35 pregnancies to obtain fetal cells for DNA linkage study, for which Southern blotting and DNA hybridization were used to detect seven restriction fragment length polymorphisms (RFLPs) within the β-globin gene cluster: ϵ-HincII, Gγ-HindIII, Aγ-HindIII, Φβ-HincII, 3′Φβ-HincII, β-AvaII, and 3′β BarnHI. β-Thalassaemia major was diagnosed in seven and excluded in 22 pregnancies. In the remaining six cases, β-thalassaemia major could not be excluded. In these six pregnancies and another one with late booking, ultrasound-guided cordocentesis was performed at the 22nd to 27th week of gestation. Globin chain composition was determined with urea-acetic acid-Triton X-100-12 per cent polyacrylamide gel electrophoresis. β-Thalassaemia major was diagnosed in two fetuses and excluded in the other five. Eleven fetuses (in which β-thalassaemia major was excluded) have been delivered and are healthy at more than 5 months old DNA linkage analysis coupled with globin chain electrophoresis provides an effective way for prenatal diagnosis of β-thalassaemia major, although these methods are being replaced by more direct detection techniques using oligonucleotide probes. 相似文献
13.
14.
In this paper we describe the use of five-colour fluorescence in situ hybridization for prenatal diagnosis of aneuploidy using uncultured amniotic fluid cells. The analysis is based on ratio mixing of dual-labelled probes and digital imaging for the detection and visualization of five different probes specific for the five target chromosomes, 13, 18, 21, X, and Y. A retrospective blind analysis of 30 coded uncultured amniotic fluid samples correctly detected fetal sex and five trisomy 21 cases. Multicolour fluorescence in situ hybridization used in this way allows rapid and simultaneous detection of the most frequent aneuploidies. 相似文献
15.
An amniocentesis was performed at 13.3 weeks' gestation for advanced maternal age. A mosaic sex chromosome pattern was found: of 50 cells examined, 34 had a 45,X karyotype. In 14 cells with a modal number of 46, a recognizable Y was substituted by a small non-fluorescent marker. C-banding identified the marker as an isodicentric in 12 cells. In two cells, the non-fluorescent marker appeared to be monocentric and looked like a non-fluorescent del (Yq), but could have been an isodicentric Y with inactivation of one of the centromeres. Two cells with a modal number of 47 showed two copies of the monocentric marker. Fluorescent in situ hybridization with an alpha satellite Y-specific centromeric probe confirmed the Y-chromosome origin of the markers and allowed for more accurate prenatal diagnostic information. 相似文献
16.
基于TMS320F240 DSP的电网谐波抑制全数字化控制的研究 总被引:1,自引:0,他引:1
为治理电力系统谐波污染 ,提高电力系统运行的安全稳定性 ,笔者对混合式并联有源电力滤波系统的结构和工作原理进行了分析和研究。该系统由无源滤波器和小容量有源滤波器串联构成 ,与被补偿的谐波负载并联连接。基于TMS32 0F2 4 0DSP开发套件 ,设计数字控制器主程序和中断服务子程序。实验结果表明 ,采用TMS32 0F2 4 0DSP编程方便并直观 ,运算精度高 ,控制器参数调整方便 ,易于实现各种控制策略。研究成果对DSP在有源电力滤波系统中的应用有重要参考价值。 相似文献
17.
信息时代的数字图书馆 总被引:2,自引:0,他引:2
数字图书馆是将文字、图像、声音等信息数字化,通过国际互联网传输,做到信息资源全球共享.数字图书馆拥有丰富的多种媒体数字化信息资源,是没有时空限制的超大规模知识中心. 相似文献
18.
如何发挥高校图书馆在大学生诚信教育中的作用 总被引:3,自引:0,他引:3
诚信缺失的造成有政府、社会、学校、家庭方面的客观原因,也有大学生自身的主观原因.加强大学生诚信教育是高等教育不可或缺的重要组成部分,具有教育职能的高校图书馆应充分发挥其教育职能作用.图书馆员要塑造良好的诚信形象,营造诚信氛围,开展诚信服务,聘请专家作诚信教育讲座,建立读者诚信档案等. 相似文献
19.
如何实现广播电视网节目内容的自动审计已成为目前广播电视网网络安全所面临的问题。笔者首先分析了目前有线电视网节目内容的审计,大多基于人工,不仅费时费力,且与先进的现代电视技术极不相匹配,接着对图像内容过滤的关键技术进行了分析,并用反差、能量、熵、相关4个特征量表示纹理特征,在此基础上用交互的动态规划法来进行图像对象分割然后利用相似度描述两个特征量的相似程度,最后以对黄色内容识别为实例,通过提取图像的纹理特征与定义好的特征库相匹配,监控包含不良人体图像信息的视频,实现对有线电视网节目内容的自动审计。 相似文献
20.
极性基湿润剂与矿岩类粉尘颗粒的作用机理 总被引:4,自引:0,他引:4
根据矿岩类粉尘微颗粒的表面性质和极性基湿润剂的特性,应用分子热力学和表面物理化学理论探讨了湿润型抑尘剂与矿岩类粉尘之间的相互作用机理。矿岩类粉尘吸附水的本质是由于它们之间的相互吸引作用,是分子之间的短程相互作用力和长程作用力共同作用的结果。当矿岩类粉尘与水相碰撞时,只有当吸引力大于排斥力时,水分子才能牯附于矿岩类粉尘,表面张力和体系自由能足够小时,水才能湿润矿岩类粉尘。分析了湿润剂对水和矿岩类粉尘的表面改性的原理,它增加水和矿岩类粉尘之间的相互作用力,减小了界面表面张力和体系自由能,使矿岩粉尘被水湿润能自由地进行。 相似文献