Mycoremediation of diclofenac using Mucor hiemalis

Diclofenac (DCF) is a pharmaceutical drug widely found in the aquatic environment, where it represents a persistent, anthropogenic hazard to all biota. Owing to the reported inefficiency of water treatment strategies to remove pharmaceuticals, the present study aimed to investigate the ability of the aquatic fungus Mucor hiemalis to take up and accumulate DCF. Cultures of M. hiemalis were exposed to varying concentrations of DCF (10, 25 and 50 µg/L) over a period of 144 h. In the presence of M. hiemalis, DCF concentrations in the media decreased by 95% within 24 h. This early removal was most likely due to extracellular metabolism of DCF, as low proportions of the pharmaceutical were found in the mycelium of the fungus, thereby excluding bioaccumulation as the main mode of removal. However, adsorption of DCF onto the surface of the M. hiemalis pellets cannot be excluded. Our study provides the first report of DCF remediation using M. hiemalis and is the first successful step towards a feasible and inexpensive bioremediation technique for DCF.     

上海郊区三类典型生境的两栖类分布特征

上海郊区农田,林地,湿地(池塘)三类典型生境是两栖类最主要的栖息地,生境的差异以及两栖类生态特征的不同造成了两栖类在分布上的差异。2014年和2016年5~8月对上海郊区三类典型生境的30个研究样点两栖类生物多样性进行调查,并运用单因素方差分析对三类生境的两栖类群落结构进行比较,通过层次聚类法分析不同生境的两栖类组成相似性。结果显示:(1)两年间共记录到上海郊区两栖类动物1目4科4属5种5 397只,其中泽陆蛙(Fejervarya multistriata),金线侧褶蛙(Pelophylax plancyi)为优势种。黑斑侧褶蛙(Pelophylax nigromaculata),中华蟾蜍(Bufo gargarizans),饰纹姬蛙(Microhyla fissipes)为常见种。这5种两栖类都为广型分布种。(2)三类生境的两栖类物种丰富度无显著性差异,而两栖类多度差异显著。(3)泽陆蛙,饰纹姬蛙的种群密度在三类生境中差异显著,且在农田生境中最高。黑斑侧褶蛙,金线侧褶蛙的种群密度在三类生境中差异显著且在池塘生境中最高。中华蟾蜍在三类生境中的种群密度无显著性差异。(4)聚类结果为30个研究样点中林地生境与池塘生境被聚为一类,农田生境被单独聚为一类。建议通过对不同生境的保护与恢复达到对上海郊区两栖类生物多样性保护的目的。     

Differences in sensitivity to the fungal pathogen Batrachochytrium dendrobatidis among amphibian populations

Contributing to the worldwide biodiversity crisis are emerging infectious diseases, which can lead to extirpations and extinctions of hosts. For example, the infectious fungal pathogen Batrachochytrium dendrobatidis (Bd) is associated with worldwide amphibian population declines and extinctions. Sensitivity to Bd varies with species, season, and life stage. However, there is little information on whether sensitivity to Bd differs among populations, which is essential for understanding Bd‐infection dynamics and for formulating conservation strategies. We experimentally investigated intraspecific differences in host sensitivity to Bd across 10 populations of wood frogs (Lithobates sylvaticus) raised from eggs to metamorphosis. We exposed the post‐metamorphic wood frogs to Bd and monitored survival for 30 days under controlled laboratory conditions. Populations differed in overall survival and mortality rate. Infection load also differed among populations but was not correlated with population differences in risk of mortality. Such population‐level variation in sensitivity to Bd may result in reservoir populations that may be a source for the transmission of Bd to other sensitive populations or species. Alternatively, remnant populations that are less sensitive to Bd could serve as sources for recolonization after epidemic events.     

Properties of mercuric reductase from a HgCl2-resistant fungus

Mercuric reductase is detected in Cephalosporium tabacinum F2.The enzyme, which is an intracellular one,catalyzes the reduction of mercuric ions to elemental mercury,which requires NADH as an electron donor and added sulfhydryl compound.The optimum temperature and pH of the enzymatic reaction are 30℃ and 7.0- 8. 0,respectively.The enzyme activity is stable in the range of 25- 30℃ for 40min,and stable at pH 7. 0 for 2 hours. Metal ions such as Ag⁺ ,CO²⁺), Cu²⁺,Zh²⁺, Mn²⁺,Ni²⁺ show different degrees of inhibitory effect on the enzyme activity,other compounds such as phenylmercury acetate and potassium ferricyanide also partially inhibit the enzyme activity.     

Effects of copper, manganese and pH on the growth and several enzyme activities of mycorrhizal fungus Cenococcum geophilum Fr.

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电子产品常用有机涂层防霉特性研究

对于电子产品中不防霉的材料和零部件,为防止其长霉影响整个产品,采用防霉涂层和喷涂保护剂是最经济有效的措施.通过对1280件电子产品(或主要组成材料和工艺试片)霉菌试验结果的统计分析,总结出各种电子产品常用材料,特别是几种常用有机涂层的防霉特性.     

真菌(Aspergillus sp.)吸附Cu2+的研究

利用真菌Aspergillus sp.制备生物吸附剂,吸附重金属离子铜,实验分析了影响吸附率和吸附量的因素,结果表明,优化条件下,30℃, pH值5.0,起始Cu2+浓度50mg/L,摇床转速150r/min,经1mol/L NaOH预处理的吸附剂用量为5g/L,吸附时间45min,该吸附剂对Cu2+的吸附率达到92.22%.通过细胞显微观察和X-射线光电子能谱技术手段推断该菌细胞壁是Cu2+ 吸附的主要部位.     

Colonial recognition of fungus in the fungus-growing ant Acromyrmex subterraneus subterraneus (Hymenoptera: Formicidae)

Summary. Leaf cutting ants live in symbiosis with a basidiomycete fungus that is exploited as a source of nutrients for the ant larvae. Tests of fungus transport demonstrated that Acromyrmex subterraneus subterraneus workers discriminate concolonial fungus from alien fungus, and rejected the latter. Larvae and pupae of the ant were used as controls. Chemical analysis of the fungus revealed a great similarity between its hydrocarbon profile and that found on the ant brood. Experiments with lures showed that chemical extracts from the fungus are responsible for this discrimination process. Moreover, the presence of brood inside the fungus seemed to be important for discrimination of the fungus by workers. Resident workers accepted concolonial broodless fungus less than concolonial fungus inoculated with brood odor. Fungus seems to acquire colonial odor passively, simply by contact with the brood. The impact of fungus volume present in the nest on closure of the colony is discussed. We show here for the first time the importance of a symbiotic vegetal organism in colonial recognition in social insects. Received 14 April 2000; accepted 29 September 2000     

两栖动物种群衰退研究进展

过去50a中，全球范围内许多两栖动物种群显著衰退，一些种类已经灭绝．研究发现，这类现象的出现与人类活动日益加强密切相关，生态环境的破坏，污染的排放、气候的改变、野生动植物疾病的流行等因素是导致这一现象的主要原因.本文总结介绍了近年来国际上本领域的最新研究成果与动态，并对我国的研究现状和未来作了讨论．图1参40     

Cost‐effective conservation of an endangered frog under uncertainty

How should managers choose among conservation options when resources are scarce and there is uncertainty regarding the effectiveness of actions? Well‐developed tools exist for prioritizing areas for one‐time and binary actions (e.g., protect vs. not protect), but methods for prioritizing incremental or ongoing actions (such as habitat creation and maintenance) remain uncommon. We devised an approach that combines metapopulation viability and cost‐effectiveness analyses to select among alternative conservation actions while accounting for uncertainty. In our study, cost‐effectiveness is the ratio between the benefit of an action and its economic cost, where benefit is the change in metapopulation viability. We applied the approach to the case of the endangered growling grass frog (Litoria raniformis), which is threatened by urban development. We extended a Bayesian model to predict metapopulation viability under 9 urbanization and management scenarios and incorporated the full probability distribution of possible outcomes for each scenario into the cost‐effectiveness analysis. This allowed us to discern between cost‐effective alternatives that were robust to uncertainty and those with a relatively high risk of failure. We found a relatively high risk of extinction following urbanization if the only action was reservation of core habitat; habitat creation actions performed better than enhancement actions; and cost‐effectiveness ranking changed depending on the consideration of uncertainty. Our results suggest that creation and maintenance of wetlands dedicated to L. raniformis is the only cost‐effective action likely to result in a sufficiently low risk of extinction. To our knowledge we are the first study to use Bayesian metapopulation viability analysis to explicitly incorporate parametric and demographic uncertainty into a cost‐effective evaluation of conservation actions. The approach offers guidance to decision makers aiming to achieve cost‐effective conservation under uncertainty.