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151.
152.
Andressa Gonsioroski Mary Laws Vasiliki E. Mourikes Alison Neff Jenny Drnevich Michael J. Plew Jodi A. Flaws 《环境科学学报(英文版)》2022,34(7):46-57
Iodoacetic acid (IAA) is an unregulated water disinfection byproduct that is an ovarian toxicant. However, the mechanisms of action underlying IAA toxicity in ovarian follicles remain unclear. Thus, we determined whether IAA alters gene expression in ovarian follicles in mice. Adult female mice were dosed with water or IAA (10 or 500 mg/L) in the water for 35-40 days. Antral follicles were collected for RNA-sequencing analysis and sera were collected to measure estradiol. RNA-sequencing analysis identified 1063 differentially expressed genes (DEGs) in the 10 and 500 mg/L IAA groups (false discovery rate FDR < 0.1), respectively, compared to controls. Gene Ontology Enrichment analysis showed that DEGs were involved with RNA processing and regulation of angiogenesis (10 mg/L) and the cell cycle and cell division (500 mg/L). Pathway Enrichment analysis showed that DEGs were involved in the phosphatidylinositol 3-kinase and protein kinase B (PI3K-Akt), gonadotropin-releasing hormone (GnRH), estrogen, and insulin signaling pathways (10 mg/L). Pathway Enrichment analysis showed that DEGs were involved in the oocyte meiosis, GnRH, and oxytocin signaling pathways (500 mg/L). RNA-sequencing analysis identified 809 DEGs when comparing the 500 and 10 mg/L IAA groups (FDR < 0.1). DEGs were related to ribosome, translation, mRNA processing, oxidative phosphorylation, chromosome, cell cycle, cell division, protein folding, and the oxytocin signaling pathway. Moreover, IAA exposure significantly decreased estradiol levels (500 mg/L) compared to control. This study identified key candidate genes and pathways involved in IAA toxicity and can help to further understand the molecular mechanisms of IAA toxicity in ovarian follicles. 相似文献
153.
Treatments of estrogens such as Estrone(E1), Estradiol(E2) and Ethinylestradiol(EE2) were conducted using an electrolytic reactor equipped with multi-packed granular glassy carbon electrodes.Experimental results showed that E1, E2 and EE2 were oxidized in the range of 0.45–0.85 V and were removed through electro-polymerization. Observed data from continuous experiments were in good agreement with calculated results by a mathematical model constructed based on mass transfer limitation. In continuous treatment of trace estrogens(1 μg/L), 98% of E1, E2 and EE2 were stably removed. At high loading rate(100 μg/L), removal efficiency of E1 was kept around 74%–88% for21 days, but removal efficiency reduced due to passivation of electrodes. However, removal efficiency was recovered after electrochemical regeneration of electrodes in presence of ozone. Electric energy consumption was observed in the range of 1–2 Wh/m3. From these results, we concluded that the present electrochemical process would be an alternative removal of estrogens. 相似文献
154.
对国内的饮用水水源地内分泌干扰物污染现状进行总结,发现我国饮用水水源地很多地区都存在着内分泌干扰物的污染,由于地域的不同,污染物的种类存在差异,但有机氯农药和多氯联苯类化合物的污染尤为严重。同时对水源地内分泌干扰物的种类、来源及生物学效应进行综述,并对内分泌干扰物的毒性作用机制进行了归纳,发现不同来源的内分泌干扰物,毒性作用机制不同,且一种内分泌干扰物可能同时存在多种作用机制。 相似文献
155.
为了探讨蛋白质组学技术在环境雌激素分析上的应用,用浓度为3.7×10-9mol/L的17β-雌二醇(E2)处理MCF-7细胞2或3天后进行双向电泳,对比不同的蛋白质提取、染色方法,利用光密度扫描仪将电泳图像数字化,并使用PDQuest软件进行图像分析,以观察雌激素诱导下的蛋白质表达图谱。结果表明,在蛋白质的提取上,冻融法比超声裂解法更有效;在蛋白质染色上,银染法比考马斯亮蓝染色法能得到更多可显示的蛋白质斑点。用E2处理72h后的MCF-7细胞,可检测到40个下调蛋白和5个上调蛋白。该蛋白质表达模式的改变提示了环境雌激素诱导下的特异性蛋白质表达图谱变化。本研究为分析雌激素类内分泌干扰物的内在作用机制提供了一种新的可行方法,也为建立其高通量筛选方法提供了新的思路。 相似文献
156.
Keiter S Rastall A Kosmehl T Wurm K Erdinger L Braunbeck T Hollert H 《Environmental science and pollution research international》2006,13(5):308-319
Goals, Scope and Background Fish populations, especially those of the grayling (Thymallus thymallus), have declined over the last two decades in the
upper Danube River between Sigmaringen and Ulm, despite intensive and continuous stocking and improvement of water quality
since the 1970s. Similar problems have been reported for other rivers, e.g. in Switzerland, Great Britain, the United States
and Canada. In order to assess if ecotoxicological effects might be related to the decline in fish catch at the upper Danube
River, sediment, suspended matter and waste water samples from sewage treatment plants were collected at selected locations
and analyzed in a bioanalytical approach using a battery of bioassays. The results of this pilot study will be used to decide
if a comprehensive weight-of-evidence study is needed.
Methods Freeze-dried sediments and suspended particulate matters were extracted with acetone in a Soxhlet apparatus. Organic pollutants
from sewage water were concentrated using XAD-resins. In order to investigate the ecotoxicological burden, the following bioassays
were used: (1) neutral red assay with RTL-W1 cells (cytotoxicity), (2) comet assay with RTLW1 cells (genotoxicity), (3) Arthrobacter
globiformis dehydrogenase assay (toxicity to bacteria), (4) yeast estrogen screen assay (endocrine disruption), (5) fish egg
assay with the zebrafish (Danio rerio; embryo toxicity) and (6) Ames test with TA98 (mutagenicity).
Results and Discussion The results of the in vitro tests elucidated a considerable genotoxic, cytotoxic, mutagenic, bacteriotoxic, embryotoxic and
estrogenic burden in the upper Danube River, although with a very inhomogeneous distribution of effects. The samples taken
from Riedlingen, for example, induced low embryo toxicity, but the second highest 17β-estradiol equivalent concentration (1.8
ng/L). Using the fish egg assay with native sediments, a broad range of embryotoxic effects could be elucidated, with clear-cut
dose-response relationships for the embryotoxic effects of contaminated sediments. With native sediments, embryotoxicity was
clearly higher than with corresponding pore waters, thus corroborating the view that – at least for fish eggs – the bioavailability
of particle-bound lipophilic substances in native sediments is higher than generally assumed. The effect observed most frequently
in the fish egg assay was a developmental delay. A comparison of our own results with locations along the rivers Rhine and
Neckar demonstrated similar or even higher ranges of ecotoxicological burdens in the Danube River.
Conclusions The complex pattern of ecotoxicological effects caused by environmental samples from the Danube River, when assessed in an
in vitro biotest battery using both acute and more specific endpoints, showed that integration of different endpoints is essential
for appropriate hazard assessment. Overall, the ecotoxicological hazard potential shown has indeed to be considered as one
potential reason for the decline in fish catches at the upper Danube River. However, based on the results of this pilot study,
it is not possible to elucidate that chemically induced alterations are responsible for the fish decline.
Recommendations and Perspective . In order to confirm the ecological relevance of the in vitro results for the situation in the field and especially for the
decline of the grayling and other fishes, further integrated investigations are required. For linking the weight of evidence
obtained by in vitro assays and fish population investigations, the application of additional, more specific biomarkers (e.g.
vitellogenin induction, EROD and micronucleus assay) has been initiated in fish taken from the field as well as in situ investigations. 相似文献
157.
利用MCF-7细胞增殖实验、细胞周期分析、细胞雌激素受体水平测定和断乳大鼠子宫增重实验探讨了邻苯二甲酸二乙基己酯(DEHP)和邻苯二甲酸二丁酯(DBP)内分泌干扰活性及其可能的作用机制.结果表明,DEHP和DBP可诱导MCF-7细胞增殖、细胞内雌激素受体水平增高,对细胞周期无明显影响,DEHP不引起断乳大鼠子宫增重.推断DEHP和DBP可能并不是通过模拟雌激素而是引起体内天然激素受体水平改变等其他机制影响机体内分泌系统. 相似文献
158.