Analysis of the effects diclofenac has on Japanese medaka (Oryzias latipes) using real-time PCR

The expression levels of cytochrome P450 1A, p53 and vitellogenin were investigated in three different tissues of male medaka fish after exposure to diclofenac that is one of the main concerns among pharmaceuticals frequently found in sewage treatment plant (STP) effluents. The results showed that cytochrome P450 1A, p53 and vitellogenin were highly expressed in tissue-specific gene expression patterns after exposure to 8 mg/l and 1 μg/l of diclofenac. These elevated expression levels of three biomarkers suggested that diclofenac has potential to cause cellular toxicity, p53-related genotoxicity and estrogenic effects. It is also noteworthy that diclofenac has the potential to cause these effects even at an environmentally relevant concentration of diclofenac, 1 μg/l.     

上海环境空气质量预报考核评分方法研究和应用

根据环境空气质量新标准和技术规范的要求,结合上海空气质量预报工作的实际,制定了一套环境空气质量预报考核评分方法,为空气质量预报结果的考核和预报工作的改进提供了定量依据,并在上海得到实际应用。该方法基于空气质量指数评价方法,重点对未来24 h的分段预报（当日夜间20：00至次日6：00、次日上午6：00—12：00、次日下午12：00—20：00）结果进行考核,区分优级时段和非优级时段,侧重对污染日的评估并突出首要污染物的评分权重,并增加级别变化趋势的描述评分和污染加分项。     

荧光定量PCR技术在环境领域的应用

实时荧光定量PCR技术作为一项新兴技术,越来越受到人们的重视,它以快速、准确定量、便捷等优点广泛应用于科学研究各个领域。近年来,实时荧光定量PCR技术不断发展,并应用于环境领域中,大大提高了环境监测水平。综述了实时荧光定量PCR技术的基本原理及其在国内外环境领域中的应用。