Number of COVID-19 cases required in a population to detect SARS-CoV-2 RNA in wastewater in the province of Alberta, Canada: Sensitivity assessment

With a unique and large size of testing results of 1,842 samples collected from 12 wastewater treatment plants (WWTP) for 14 months through from low to high prevalence of COVID-19, the sensitivity of RT-qPCR detection of SARS-CoV-2 RNA in wastewater that correspond to the communities was computed by using Probit analysis. This study determined the number of new COVID-19 cases per 100,000 population required to detect SARS-CoV-2 RNA in wastewater at defined probabilities and provided an evidence-based framework of wastewater-based epidemiology surveillance (WBE). Input data were positive and negative test results of SARS-CoV-2 RNA in wastewater samples and the corresponding new COVID-19 case rates per 100,000 population served by each WWTP. The analyses determined that RT-qPCR-based SARS-CoV-2 RNA detection threshold at 50%, 80% and 99% probability required a median of 8 (range: 4-19), 18 (9-43), and 38 (17-97) of new COVID-19 cases /100,000, respectively. Namely, the positive detection rate at 50%, 80% and 99% probability were 0.01%, 0.02%, and 0.04% averagely for new cases in the population. This study improves understanding of the performance of WBE SARS-CoV-2 RNA detection using the large datasets and prolonged study period. Estimated COVID-19 burden at a community level that would result in a positive detection of SARS-CoV-2 in wastewater is critical to support WBE application as a supplementary warning/monitoring system for COVID-19 prevention and control.     

一台延期报废的氧气储罐的监控使用

通过对一台储氧卧罐检验结果的分析及对历次检验情况的比较,建议对这台容器进行判废处理。但由于生产需要,该容器不能立即更新,专家们根据实际情况提出了用声发射仪监控,延长使用期一年的意见。     

冷挤压双层组合凹模的最佳设计

此文应用厚壁圆筒弹性解,推导了冷挤压双层组合凹模,具有最大弹性极限时所能承受的最大挤压应力的计算公式,以及满足上述条件时双层组合凹模分界面半径和分界面过盈量的计算公式.利用上述各式,可使组合凹模获得最佳设计.     

Assessment of the use of selected rodents in ecological monitoring

Rodents can be useful in detecting environmental impacts because they are easy to study (easy to capture and handle), they can occur in densities adequate for statistical analysis, and they are ecologically important. In this study the usefulness of rodent populations for ecological monitoring was investigated by examining the effect of variation on the possibility of detecting differences among populations of rodents on 10 trapping grids. The effects of sampling frequencies and dispersal on detecting differences in population parameters among grids was also investigated, as was the possibility of inferring population parameters from correlations with habitat data. Statistically significant differences as small as 4.3Peromyscus maniculatus/ha were detected between grids. Of 10 populations, this comprised 12% of the highest-density population and 44% of the lowest-density population. Smaller and more differences among grids were found by examining only animals surviving from previous months. Dispersal confounds detection of direct impacts to populations, especially during the breeding season. Infrequent sampling fails to detect impacts that occur between sampling periods and will indicate impacts when observed changes result from natural variation. Correlations between population parameters and habitat variables exist but should only be used in predicting, not measuring, impacts. It is concluded that some rodent populations can be used in ecological monitoring. However, intensive sampling is required to account for variation and dispersal.     

Fluorescence detection of phosphate in an aqueous environment by an aluminum-based metal-organic framework with amido functionalized ligands

● A novel Al-MOF was successfully synthesized by a facile solvothermal method. ● Al-MOF showed superior performance for phosphate detection. ● High selectivity and anti-interference for detection were demonstrated. ● The high coordination between Al-O and PO₄³⁻ was the key in fluorescence sensing. The on-site monitoring of phosphate is important for environmental management. Conventional phosphate detection methods are not appropriate to on-site monitoring owing to the use of complicated detection procedures, and the consequent high cost and maintenance requirements of the detection apparatus. Here, a highly sensitive fluorescence-based method for phosphate detection with a wide detection range was developed based on a luminescent aluminum-based metal-organic framework (Al-MOF). The Al-MOF was prepared by introducing amine functional groups to conventional MIL to enhance phosphate binding, and exhibited excellent fluorescence properties that originated from the ligand-to-metal charge transfer (LMCT). The detection limit was as low as 3.25 μmol/L (0.10 mg/L) and the detection range was as wide as 3–350 μmol/L (0.10–10.85 mg/L). Moreover, Al-MOF displayed specific recognition toward phosphate over most anions and metal cations, even for a high concentration of the co-existent ions. The mechanism of phosphate detection was analyzed through the characterization of the combination of Al-MOF and phosphate, and the results indicated the high affinity between Al-O and phosphate inhibited that the LMCT process and recovered the intrinsic fluorescence of NH₂-H₂BDC. The recovery of the developed detection method reached a satisfactory range of 85.1%–111.0%, and the feasibility of on-site phosphate detection was verified using a prototype sensor for tap water and lake water samples. It was demonstrated that the prepared Al-MOF is highly promising for on-site detection of phosphate in an aqueous environment.     

火灾烟雾光学探测技术原理的数学分析

从辐射传热的角度，同时考虑照明条件下的火灾烟雾与环境背景中其他物体的辐射，采用信／噪比处理方法，对火灾烟雾光学探测原理进行了数学分析，分析指出：火灾烟雾光学探测必然分为散射型和透过型两种原理类型，以及存在着四种技术原理。     

弹塑性极限平衡法分析复杂岩质边坡的稳定性

笔者采用弹塑性极限平衡法来研究分析复杂岩质边坡的稳定性 ,即以塑性极限理论为基础 ,应用逐渐降低材料强度来逼近系统的极限平衡 ,并以屈服区的贯通来表征系统的极限平衡状态。此时 ,系统达到临界平衡状态时所对应的材料强度降低倍数即为整体最小安全系数 ,临界平衡状态定义为边坡内的塑性屈服区能够形成潜在的滑移通道时的状态。以城门山露天矿南帮边坡为研究对象 ,通过自行改编的NCAP -FS程序进行了数值分析 ,结果表明 ,南帮边坡现阶段是安全稳定的 ,与现场观测结果一致。     

煤巷锚杆支护结构的可靠性分析

根据煤巷围岩力学参数中有许多随机变量的特征 ,笔者应用工程结构可靠性理论 ,提出并建立了煤巷锚杆支护结构可靠性分析模型 ,得到了其可靠度的计算公式 ,提出了提高支护结构可靠度的措施 ,为优化煤巷锚杆支护参数提供了一种新方法。这些研究成果对指导煤巷支护设计 ,减少支护失效事故 ,保证矿井正常安全生产具有重要的意义。     

SOS法检测环境中遗传毒物的应用和研究

随着社会工业化水平的日益提高,从各种渠道进入环境中的化学污染物的种类和数量也在急剧增加,污染物中不少具有致突变性、致畸性和致癌性,其存在能直接影响到人类的健康和安全,急需发展更为灵敏、经济和快速的检测方法来确定遗传毒物在环境中的存在和数量.在近年来建立的各种生物检测系统中,SOS 检测法以其独特的生物学原理和准确性而受到重视.本文从 SOS 法的原理、分类及优缺点等方面综述了其应用于环境检测的研究进展.     

餐饮业油烟监测中需注意的问题

提出在加强对餐饮业环境管理的同时,务必注意油烟监测中的技术问题。指出油烟测试中,不锈钢滤筒优于刚玉滤筒;应用红外分光法测定油烟含量,不受油品成分限制,结果较准确、可比。由于厨房的实际工作灶头数不同,每个灶头发热功率的不同和每个灶头产生油烟量的不同,必须把实际工作灶头数换算为折算工作灶头数,才具有一致性和可比性。折算工作灶头数n=(工作灶头数/实际灶头数)×(排风罩面积/1.1)。