Subcellular localization of copper in tolerant and non-tolerant plant

The ability of Elsholtzia splendens Naki( E. splendens) to accumulate copper appears to be governed by its high degree of copper tolerance. However, the tolerance mechanism on the physiological basis is unknown. Using transmission electron microscope (TEM) and energy dispersive analysis of X-rays(EDX), the likely location of copper within the cells of the tolerant and non-tolerant was determined. Here the role of vacuolar and cell wall compartmentalization in this copper tolerant plant were investigated. A direct comparison of copper locations of E. splendens and the non-tolerant Astragalus sinicus L. ( A. sinicus ) showed that the majority of copper in the tolerant was localized primarily in the vacuolar, cell wall, on the plasmamembrane, beside lipid grains induced by copper pollution, in the chloroplasts and amyloids; but in the non-tolerant, copper precipitates only be observed on the plasmamembrane, in the chloroplasts and cytoplasm under copper exposure conditions that were toxic to both species. This revealed that the tolerant accumulates more copper in the vacuole and cell wall than the non-tolerant, where was regarded as the storage compartment of tolerant plant or hyperaccumulator for heavy metals.     

鼠Tcp11l1基因的结构与表达分析

为了分析鼠Tcp11l1基因的结构和表达,从小鼠睾丸组织总cDNA中扩增鼠Tcp11l1基因的开读框架(Open reading frame,ORF),定向克隆到真核表达载体pEGFP-N1,构建融合表达质粒pTcp11l1-EGFP,转染HEK293细胞,荧光显微镜下观察Tcp11l1基因的亚细胞定位;设计跨小鼠Tcp11l1基因两个外显子的引物,RT-PCR分析此基因在小鼠各组织中的表达情况.并利用生物信息学的方法对鼠Tcp11l1基因的结构进行初步预测.转染pTcp11l1-EGFP后在胞质中能明显看到绿色荧光信号,而在胞核和胞膜中无绿色荧光信号,表明鼠Tcp11l1蛋白定位于细胞质;RT-PCR分析结果表明,鼠Tcp11l1基因在小鼠各组织中广泛表达.生物信息学结果表明,鼠Tcp11l1和鼠Tcp11的蛋白具有相同的TCP11结构域,不能确定是否存在跨膜序列.鼠Tcp11l1基因和鼠Tcp11基因具有相似的亚细胞定位和TCP11结构域,表明这两个基因可能具有相似的受体功能.但是与Tcp11特异表达于睾丸组织的延长精细胞和精子不同,Tcp11l1为广泛表达,说明Tcp11l1可能在多种组织细胞中发挥作用.     

我国普及推广循环流化床锅炉的必要性及推广设想

循环流化床锅炉在环保、节能等方面效果显著,在我国推广应用有重大意义,文章对该技术在我国推广普及的必要性及基本原则和思路进行了论述.     

重庆市主城区农业源氨排放研究

对重庆主城区畜禽养殖业氨(NH3)排放因子进行了本地化修正,估算了农业源NH3排放量。主城区农业源年排放NH38 482 t,其中化肥施用NH3排放量最大,占总排放量的66.7%;畜牧养殖业占26.3%;农作物释放占6.9%。巴南区农业源NH3排放量最大,占主城区排放总量的41%,渝中区为零排放。主城区农业源NH3排放强度为1 552.1kg/km2,大渡口区排放强度最高。主城区畜禽养殖业NH3排放比例低于全国平均水平,化肥施用NH3排放比例高于全国平均水平,但与全国农业源NH3排放结构类似。     

地质体材料剪切带内部的常剪切应变点及速度分布分析

对于峰后线性应变软化的地质体材料,剪切带内部的总剪切应变等于弹性剪切应变(由经典弹性理论描述)及由微结构效应而引起的局部塑性剪切应变(由非局部理论或梯度塑性理论描述)之和。若剪切应力—塑性剪切应变曲线的斜率的绝对值(称之为软化模量)小于剪切弹性模量的两倍,则在剪切带的任一剖面内存在两个总剪切应变不依赖于剪切应力的点,称之为常剪切应变点。在这两个点上,弹性剪切应变的降低和局部塑性剪切应变的增加处于平衡状态,总剪切速度达到它的最大值或最小值。在两个常剪切应变点之间,局部总速度随剪切应力率的降低而增加。剪切带内部的局部总速度分布是非线性的,这与通常采用的剪切带内部速度的线性分布假定(忽略微结构效应)不同。