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11.
The possibility that 3T3-L1 preadipocytes, while the level of its glucose uptake activity is relatively low, may offer a useful tool for studying the cause for 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced "lipolytic response" was studied. It was established first, that TCDD causes reduction of glucose uptake, one of the hallmark events of the lipolytic process. Then the function of c-Src was investigated. The antisense c-src oligonucleotide decreased the inhibitory action of TCDD on glucose uptake activity in a sequence specific manner. Since antisense oligonucleotides are known to own their blocking effects to their ability to reduce translation of proteins, Western blotting analysis was performed to verify their effectiveness. As expected, the treatment of pre-adipocytes with antisense c-src oligonucleotide reduced c-Src in a sequence specific manner. The treatment of antisense c-src oligonucleotide alone was sufficient to diminish the inhibitory action of TCDD on glucose uptake activity in 3T3-L1 cells, indicating that c-Src is somehow involved in the action of TCDD. In a similar manner, the contribution of c-Fos was investigated using antisense c-fos oligonucleotide, since c-Fos is known to be one of the most affected proteins by c-Src activation among AP-1 members. The treatment of antisense c-fos oligonucleotide did not block the effect of TCDD on glucose uptake activity in 3T3-L1 cells. Therefore, it is unlikely that c-Fos is very important in the lipolytic signal transduction of TCDD mediated through c-Src. In order to determine the relationship between c-Src and c-Myc in the mitotic signal transduction pathway, the effect of antisense c-myc oligonucleotide was investigated. Basically the same result as antisense c-src oligonucleotide experiment was obtained thereby, suggesting the importance of c-Myc as well as c-Src in the signal transduction of TCDD. To show the effect of antisense c-myc oligonucleotide treatment, the level of c-Myc protein by Western blotting and electrophoretic gel-mobility shift assay was assessed. However, antisense c-myc oligonucleotide treatment increased the activity of c-Myc in a sequence specific manner. This may be the result of cellular compensatory response to the initial suppression of c-Myc by antisense treatment. The observation that antisense c-fos oligonucleotide could not block the effect of TCDD indicates that this preadipocyte model is different from the adipocyte differentiation model. 相似文献
12.
Shigetaka Seki Fumio Osakada Toshiaki Yoshioka 《Journal of Material Cycles and Waste Management》2014,16(3):385-397
To facilitate recycling, not only R&D but also the creation of efficient recycling systems—collection and processing of recyclates as well as finding appropriate end-uses—is essentially important. PVC has a particular advantage in regards to mechanical recycling when compared to other major plastics. However, there is no one-size-fits-all approach to a solution. Specific situations of used or off-cut products and their applications need to be well studied. Vinyl industry in Japan has devoted itself for many years to the promotion of recycling of vinyl products. It started an R&D support program in 2007 and seven projects have been completed since then. Some of the new developments include mechanical recycling for products like wall covering and tarpaulin which were considered difficult to recycle in the past. Progress in chemical recycling has been made with a technology for the removal of chlorine from shredder dusts. Recovery of chlorine is a challenge for the future. Collaboration among the various players involved in the recycling of a specific product is essential for obtaining a successful result. 相似文献
13.
Relation between mechanochemical dechlorination rate of polyvinyl chloride and mill power consumption 总被引:1,自引:0,他引:1
William Tongamp Junya Kano Yuichiro Suzuta Fumio Saito Nickolas J. Themelis 《Journal of Material Cycles and Waste Management》2009,11(1):32-37
A sample of polyvinyl chloride (PVC) powder was milled with CaO powder in a planetary mill for various mill operational parameters.
The milled product consisted of dechlorinated hydrocarbon and water-soluble CaOHCl. The dechlorination rate of PVC was determined
by the concentration of Cl− ions measured in solution after dispersing the milled product in water. To evaluate the power consumption during PVC dechlorination,
the mill power consumption was measured during each experimental run. In addition, media motion during planetary milling was
simulated using the discrete element method (DEM), enabling calculation of the mill power consumption. The power consumption
calculated by the DEM simulation compared well with the power consumption measured experimentally. The dechlorination rate
correlated well with the specific mill power consumption, regardless of the sample weight. The dechlorination rate of PVC
when milled with oyster shells (CaCO3) was observed to be faster than that of the PVC/CaO system, and oyster shells could be used as a reactant for the treatment
of PVC wastes. This work should be useful for the design of a reactor for the dechlorination of PVC.
Chemical Feedstock Recycling & Other Innovative Recycling Techniques 6 相似文献
14.
15.
A simple mathematical fate model, Multi‐Phase Non‐Steady State Equilibrium Model (MNSEM) is proposed to evaluate distribution, persistence, and concentrations of chemicals in a model environment consisting of air, water, soil and sediment phases. The model is applied to evaluation of environmental fate and concentration of trichloroethylene and 1,4‐dichlorobenzene under generic conditions representative of Japan. Evaluated chemical concentrations in air are within a factor of 3 of average values in Japanese atmosphere, and evaluated concentrations in water, sediment, or fish are greater than an order of magnitude below detection limits in real environments, so that evaluated concentrations are in reasonable agreement with environmental measurement data in Japan. Although MNSEM is not a model for site‐specific evaluation of environmental fate, results suggested that this model is an adequate method to aid in evaluation of fate of chemicals under generic environment conditions. Evaluated concentration‐profiles may be used to estimate average chemical exposure concentrations for humans and the environment. 相似文献
16.
We have found that giant hornets (Vespa mandarinia japonica) are killed in less than 10 min when they are trapped in a bee ball created by the Japanese honeybees Apis cerana japonica, but their death cannot be solely accounted for by the elevated temperature in the bee ball. In controlled experiments, hornets
can survive for 10 min at the temperature up to 47°C, whereas the temperature inside the bee balls does not rise higher than
45.9°C. We have found here that the CO2 concentration inside the bee ball also reaches a maximum (3.6 ± 0.2%) in the initial 0–5 min phase after bee ball formation.
The lethal temperature of the hornet (45–46°C) under conditions of CO2 concentration (3.7 ± 0.44%) produced using human expiratory air is almost the same as that in the bee ball. The lethal temperature
of the honeybee is 50–51°C under the same air conditions. We concluded that CO2 produced inside the bee ball by honeybees is a major factor together with the temperature involved in defense against giant
hornets. 相似文献
17.
Takashi A. Inoue Tamako Hata Kiyoshi Asaoka Tetsuo Ito Kinuko Niihara Hiroshi Hagiya Fumio Yokohari 《Die Naturwissenschaften》2012,99(12):985-998
Many butterflies acquire nutrients from non-nectar sources such as puddles. To better understand how male Papilio butterflies identify suitable sites for puddling, we used behavioral and electrophysiological methods to examine the responses of Japanese Papilio butterflies to Na+, K+, Ca2+, and Mg2+. Based on behavioral analyses, these butterflies preferred a 10-mM Na+ solution to K+, Ca2+, and Mg2+ solutions of the same concentration and among a tested range of 1?mM to 1?M NaCl. We also measured the ion concentrations of solutions sampled from puddling sites in the field. Na+ concentrations of the samples were up to 6?mM, slightly lower than that preferred by butterflies in the behavioral experiments. Butterflies that sipped the 10?mM Na+ solution from the experimental trays did not continue to puddle on the ground. Additionally, butterflies puddled at sites where the concentrations of K+, Ca2+, and/or Mg2+ were higher than that of Na+. This suggests that K+, Ca2+, and Mg2+ do not interfere with the detection of Na+ by the Papilio butterfly. Using an electrophysiological method, tip recordings, receptor neurons in contact chemosensilla inside the proboscis evoked regularly firing impulses to 1, 10, and 100?mM NaCl solutions but not to CaCl2 or MgCl2. The dose?Cresponse patterns to the NaCl solutions were different among the neurons, which were classified into three types. These results showed that Japanese Papilio butterflies puddle using Na+ detected by the contact chemosensilla in the proboscis, which measure its concentration. 相似文献
18.
A mathematical model, Simplified Approach for Fate Evaluation of Chemicals in Aquatic Systems (SAFECAS), was applied to evaluate fate of 2,4,6-trichlorophenol(TCP) in three types of aquatic environments; a river, an oligotrophic lake, and an eutrophic lake.Since phenols are dissociative substances, equilibrium constants and rate constants for transfer and transformation processes were measured or estimated as a function of pH.Results suggest that most of TCP is distributed to the water phase in aquatic environments, and is almost removed by the advective outflow in the river and by the photodegradation in lakes. 相似文献
19.
Carbon Sequestration by Carbonization of Biomass and Forestation: Three Case Studies 总被引:3,自引:0,他引:3
Makoto Ogawa Yasuyuki Okimori Fumio Takahashi 《Mitigation and Adaptation Strategies for Global Change》2006,11(2):421-436
We proposed the carbon sink project called “Carbon Sequestration by Forestation and Carbonization (CFC),” which involves biomass
utilization and land conservation by incorporating the products of biomass carbonization into the agents for soil improvement,
water purification, etc. Our purpose was to demonstrate the potential of the CFC scheme for carbon sequestration, particularly
carbon storage in soil. Case studies were conducted in both developing and developed countries. 1. In southern Sumatra, Indonesia,
88,369 Mg-C year−1 of wood residue from a plantation forest and excess bark from a pulp mill would be converted into 15,571 Mg-C year−1 of the net carbon sink by biochar for soil improvement. The fixed carbon recovery of the system is 21.0%. 2. In a semiarid
region in western Australia, the carbonization of wood residue was incorporated with multipurpose projects of a mallee eucalyptus
plantation that involved the function of salinity prevention. During the project period of 35 years, the total carbon sink
would reach 1,035,450 Mg-C with 14.0% by aboveground biomass, 33.1% by belowground biomass and 52.8% by biochar in soil. 3.
In southern Kyushu, Japan, the study was focused on the effective use of surplus heat from a garbage incinerator for carbonizing
woody materials. Sawdust of 936.0 Mg-C year−1 would be converted into the net carbon sink of 298.5 Mg-C year−1 by carbonization, with the fixed carbon recovery of the system being 31.9%. Consequently, the CFC project could encourage
the creation of a carbon sink in soil. However, we recognize that the quality standard of biochar, the stability of biochar
in soil, and the methods for monitoring biochar utilization must be clarified before incorporating biochar carbon into the
carbon credit system.
Throughout this article (except for diagrams and in citation details) carbonized biomass is, with the authors'agreement, called
‘biochar’ in lieu of the commonly used but misleading word ‘charcoal’ (Editor). 相似文献
20.
Shu Saeki Junya Kano Fumio Saito Kaoru Shimme Seiichi Masuda Tsuyoshi Inoue 《Journal of Material Cycles and Waste Management》2001,3(1):20-23
Polyvinyl chloride (–CH2–CHCl–)
n
(PVC) was ground with a powdered inorganic material (CaO, CaCO3, SiO2, Al2O3, or slag) in a planetary ball mill under atmospheric conditions to investigate the effect of additions on its dechlorination.
The grinding causes a dehydrochlorinating reaction, forming a mixture of partially dechlorinated PVC and inorganic chloride,
depending on the grinding time. The dechlorination increases as the grinding progresses, and is improved with increasing amounts
of additives. The most effective additive is a mixture of CaO, SiO2, and Al2O3, which has the same constituent components as blast furnace slag. CaO, a mixture of CaO, SiO2, and blast furnace slag, are also effective, but CaCO3 is the least effective additive tired.
Received: August 3, 2000 / Accepted: September 21, 2000 相似文献