干旱地区几种豆科植物根瘤菌的数值分类和SDS-PAGE全细胞蛋白电泳分析

从新疆、内蒙干旱地区苜蓿属、草木樨属、锦鸡儿属植物的根瘤中分离出５４株根瘤菌，对其中４８株根瘤菌和２２株参比菌一起进行了数值分类研究，在８５％的相似性水平上，未知菌分为３个不同于已知种的新群．另加入６株分离自锦鸡儿属植物的根瘤菌，共７６株菌进行了全细胞蛋白ＳＤＳＰＡＧＥ分析，在８０％的相似性水平上，已知菌相应成群，未知菌除ＸＪ９６３４２在６８％的相似性水平上和群５、６、７、８、Ｒ．ｇａｌｅｇａ聚群外，其余未知菌则分布在４、５、６、７、８、９、１０七个群中．群４中所有菌株属于数值分类的群１；群６中有３株菌属于数值分类的群３；群９中有８株菌为数值分类群２的菌株．反映了两种方法在分类上得到的结果比较近似．     

中国与欧盟的航空安全信息分类法比较

为探讨中国和欧盟政府间进行航空安全信息交换的可行性,从中国和欧盟各自航空安全信息系统的信息结构和报告内容出发,对中国和欧盟的航空安全信息分类法进行比较。结果表明,两个分类法的信息结构是相同的,不同之处在于主题差异、部分差异和属性差异。笔者认为,虽然存在3种差异,但两个分类法都是基于国际民航组织航空安全信息分类法设计的,信息报告内容具有很大的相似性,因此,从信息内容上看,中欧进行航空安全信息交换是可行的。同时,对如何改进我国航空安全信息分类法提出了建议。     

Taxonomic Uncertainty and the Loss of Biodiversity on Christmas Island,Indian Ocean

The taxonomic uniqueness of island populations is often uncertain which hinders effective prioritization for conservation. The Christmas Island shrew (Crocidura attenuata trichura) is the only member of the highly speciose eutherian family Soricidae recorded from Australia. It is currently classified as a subspecies of the Asian gray or long‐tailed shrew (C. attenuata), although it was originally described as a subspecies of the southeast Asian white‐toothed shrew (C. fuliginosa). The Christmas Island shrew is currently listed as endangered and has not been recorded in the wild since 1984–1985, when 2 specimens were collected after an 80‐year absence. We aimed to obtain DNA sequence data for cytochrome b (cytb) from Christmas Island shrew museum specimens to determine their taxonomic affinities and to confirm the identity of the 1980s specimens. The Cytb sequences from 5, 1898 specimens and a 1985 specimen were identical. In addition, the Christmas Island shrew cytb sequence was divergent at the species level from all available Crocidura cytb sequences. Rather than a population of a widespread species, current evidence suggests the Christmas Island shrew is a critically endangered endemic species, C. trichura, and a high priority for conservation. As the decisions typically required to save declining species can be delayed or deferred if the taxonomic status of the population in question is uncertain, it is hoped that the history of the Christmas Island shrew will encourage the clarification of taxonomy to be seen as an important first step in initiating informed and effective conservation action.     

A Standard Lexicon for Biodiversity Conservation: Unified Classifications of Threats and Actions

Abstract: An essential foundation of any science is a standard lexicon. Any given conservation project can be described in terms of the biodiversity targets, direct threats, contributing factors at the project site, and the conservation actions that the project team is employing to change the situation. These common elements can be linked in a causal chain, which represents a theory of change about how the conservation actions are intended to bring about desired project outcomes. If project teams want to describe and share their work and learn from one another, they need a standard and precise lexicon to specifically describe each node along this chain. To date, there have been several independent efforts to develop standard classifications for the direct threats that affect biodiversity and the conservation actions required to counteract these threats. Recognizing that it is far more effective to have only one accepted global scheme, we merged these separate efforts into unified classifications of threats and actions, which we present here. Each classification is a hierarchical listing of terms and associated definitions. The classifications are comprehensive and exclusive at the upper levels of the hierarchy, expandable at the lower levels, and simple, consistent, and scalable at all levels. We tested these classifications by applying them post hoc to 1191 threatened bird species and 737 conservation projects. Almost all threats and actions could be assigned to the new classification systems, save for some cases lacking detailed information. Furthermore, the new classification systems provided an improved way of analyzing and comparing information across projects when compared with earlier systems. We believe that widespread adoption of these classifications will help practitioners more systematically identify threats and appropriate actions, managers to more efficiently set priorities and allocate resources, and most important, facilitate cross‐project learning and the development of a systematic science of conservation.     

Wildlife collection for scientific purposes

Illegal transfer of wildlife has 2 main purposes: trade and scientific research. Trade is the most common, whereas scientific research is much less common and unprofitable, yet still important. Biopiracy in science is often neglected despite that many researchers encounter it during their careers. The use of illegally acquired specimens is detected in different research fields, from scientists bioprospecting for new pharmacological substances, to taxonomists working on natural history collections, to researchers working in zoos, aquariums, and botanical gardens. The practice can be due to a lack of knowledge about the permit requirements in different countries or, probably most often, to the generally high level of bureaucracy associated with rule compliance. Significant regulatory filters to avoid biopiracy can be provided by different stakeholders. Natural history collection hosts should adopt strict codes of conduct; editors of scientific publications should require authors to declare that all studied specimens were acquired legally and to cite museum catalog numbers as guarantee of best practices. Scientific societies should actively encourage publication in peer-reviewed journals of work in which specimens collected from the wild were used. The International Commission on Zoological Nomenclature could require newly designated types based on recently collected specimens to be accompanied by statements of deposition in recognized scientific or educational institutions. We also propose the creation of an online platform that gathers information about environmental regulations and permits required for scientific activities in different countries and respective responsible governmental agencies and the simplification of the bureaucracy related to regulating scientific activities. This would make regulations more agile and easier to comply with. The global biodiversity crisis means data need to be collected ever faster, but biopiracy is not the answer and undermines the credibility of science and researchers. It is critical to find a modus vivendi that promotes compliance with regulations and scientific progress.     

贵州省土壤水分状况估算及分析

土壤水分是重要的土壤内在属性,亦是土壤系统分类工作中重要基础性诊断特征。将贵州省86个气象站点地面气候数据与土壤可能蒸散量模型、月干燥度和年干燥度计算公式相结合,依据中国土壤系统分类对土壤水分状况的分级要求,对贵州省土壤水分状况进行初步评判,同时探讨土壤干燥度与可能蒸散量、降水量及温度间的相互关系。结果表明,贵州省土壤水分状况包括湿润和常湿润两种类型,51个县(市)的土壤属于"湿润"水分状况,其余35个县(市)土壤属于"常湿润"水分状况。自西向东,贵州省土壤水分状况由"湿润"逐渐演变为"常湿润",中部区域为两种土壤水分状况同时存在。相较于地区性降水量,可能蒸散量对土壤干燥度影响更大;与年均气温相比,年可能蒸散量与年均地表温度相关性更强。将贵州省土壤水分状况作为诊断特性应用于土壤系统分类时,应综合分析成土环境条件。     

我国蚕豆根瘤菌的多样性和系统发育研究

采用数值分类、16S rDNA PCR-RFLP、IGS PCR-RFLP等方法对分离自我国11个省的50株蚕豆根瘤菌及11株参比菌株进行了表型测定和遗传型研究,同时对5株蚕豆根瘤菌的代表菌株进行了16S rDNA全序列测定.表型测定的结果表明,在80%的相似水平上供试菌株分为4个群,各群间存在地区交叉;16S rDNA PCR-RFLP的聚类结果与数值分类的聚类结果有很好的一致性;IGSRFLP反映的多样性更明显,形成的遗传群较多,可用于菌株间的鉴别.实验结果表明我国蚕豆根瘤菌具有极大的表型多样性和遗传多样性.系统发育研究结果表明,蚕豆根瘤菌的代表菌株均位于快生根瘤菌属(Rhizobium)系统发育分支,与R.leguminosarum USDA2370的全序列相似性达99.9%,说明蚕豆根瘤菌属于Rhizobium,系豌豆根瘤菌的一个生物型.图4表3参12     

山西吕梁山南段植物群落的数量分类和排序研究

运用TWINSPAN和DCA相结合的数量生态学方法对吕梁山南段云邱山的植物群落进行分类和排序.结果表明,TWINSPAN分类将74个样方划分为18个群丛;分类结果较好地反映了植物群落类型与环境梯度之间的关系,并在DCA二维排序图上得到较好的验证;对74个样方和优势种的DCA排序表明影响吕梁山南段植物群落分布的主导生态因子是水分和土壤,而认为干扰加剧了该区域植被的退化.图2表1参13     

内蒙古中东部地区几种豆科牧草根瘤菌的表型多样性及水分梯度对其分布的影响

选用101株分离自扁蓿豆、胡枝子、野豌豆、锦鸡儿属植物的根瘤菌菌株，进行了营养利用、抗生素抗性、耐逆性和酶活性测定等84个表型性状分析，发现分离自同种寄主植物的根瘤菌由于地理来源的不相同而存在着较大的多样性．通过数值分类，各已知种分别聚群，101株未知菌在84％的相似水平上分为4个各不同的群．根据1、2、3、4群菌的聚类情况，结合在不同水分梯度下采集的豆科牧草根瘤菌，结果发现除个别菌外，绝大部分菌株呈现随着水分梯度的不同，各菌以类群形式分布．证明土壤中水分含量对根瘤菌的分布有很大影响．