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91.
Khandaker Rayhan Mahbu Kannan Krishnan Ravi NaiduMallavarapu Megharaj 《环境科学学报(英文版)》2017,29(1):128-137
A mercury resistant bacterial strain SE2 was isolated from contaminated soil.The 16 s rRNA gene sequencing confirms the strain as Sphingopyxis belongs to the Sphingomonadaceae family of the α-Proteobacteria group.The isolate showed high resistance to mercury with estimated concentrations of Hg that caused 50%reduction in growth(EC_(50)) of 5.97 and 6.22 mg/L and minimum inhibitory concentrations(MICs) of 32.19 and 34.95 mg/L in minimal and rich media,respectively.The qualitative detection of volatilized mercury and the presence of mercuric reductase enzyme proved that the strain SE2 can potentially remediate mercury.ICP-QQQ-MS analysis of the remaining mercury in experimental broths indicated that a maximum of 44%mercury was volatilized within 6 hr by live SE2 culture.Furthermore a small quantity(23%) of mercury was accumulated in live cell pellets.While no volatilization was caused by dead cells,sorption of mercury was confirmed.The mercuric reductase gene merA was amplified and sequenced.Homology was observed among the amino acid sequences of mercuric reductase enzyme of different organisms from a-Proteobacteria and ascomycota groups, 相似文献
92.
93.
A comprehensive study on water balance, sedimentation and physico-chemical characteristics of Sagar Lake in India 总被引:1,自引:0,他引:1
Singh S Kumar B Thakural LN Galkate R 《Environmental monitoring and assessment》2009,148(1-4):265-276
In this study, an attempt has been made to work out water balance, determine rate of sedimentation and physico-chemical analysis of the lake water. The water balance is carried out using the mass balance equation to account for various input and output components. Sedimentation rates and pattern are estimated using (137)Cs and (210)Pb radiometric dating techniques. The physico-chemical analysis of the lake water is done by collecting samples from twelve locations of the lake at three different depths. The major inflow to the lake is catchment runoff, which accounts for nearly 56% of the total annual inflow and about 98% inflow takes place during monsoon period. The major outflow from the lake is weir overflow, which occurs in monsoon season only and accounts for about 85% of the total annual outflow. The estimated mean sedimentation rate in the lake is 0.58 +/- 0.028 cm/year. The estimated useful life of the lake based on post-1964 (appearance of the major peak of (137)Cs due to weapon fallout record pattern) average sedimentation rate is around 467 +/- 23 years. The lake has attained the hyper-eutrophic state due to high nitrogen and phosphorous contents in the lake water. Based on the trophic state index (TSI), the lake has become unsuitable for drinking, bathing and even for fish culture. 相似文献
94.
Comparison of in vivo and in vitro methodologies for the assessment of arsenic bioavailability in contaminated soils 总被引:2,自引:1,他引:1
An in vivo swine assay was utilised for the determination of arsenic (As) bioavailability in contaminated soils. Arsenic bioavailability was assessed using pharmacokinetic analysis encompassing area under the blood plasma-As concentration time curve following zero correction and dose normalisation. In contaminated soil studies, As uptake into systemic circulation was compared to an arsenate oral dose and expressed as relative As bioavailability. Arsenic bioavailability ranged from 6.9+/-5.0% to 74.7+/-11.2% in 12 contaminated soils collected from former railway corridors, dip sites, mine sites and naturally elevated gossan soils. Arsenic bioavailability was generally low in the gossan soils and highest in the railway soils, ranging from 12.1+/-8.5% to 16.4+/-9.1% and 11.2+/-4.7% to 74.7+/-11.2%, respectively. Comparison of in vivo and in vitro (Simplified Bioaccessibility Extraction Test [SBET]) data from the 12 contaminated soils and bioavailability data collected from an As spiked soil study demonstrated that As bioavailability and As bioaccessibility were linearly correlated (in vivo As bioavailability (mgkg(-1))=14.19+0.93.SBET As bioaccessibility (mgkg(-1)); r(2)=0.92). The correlation between the two methods indicates that As bioavailability (in vivo) may be estimated using the less expensive, rapid in vitro chemical extraction method (SBET) to predict As exposure in human health risk assessment. 相似文献
95.
Wei Nie Tao Wang Xiaomei Gao Ravi Kant Pathak Xinfeng Wang Rui Gao Qingzhu Zhang Lingxiao Yang Wenxing Wang 《Atmospheric environment (Oxford, England : 1994)》2010,44(35):4396-4403
Filter-based methods for sampling aerosols are subject to great uncertainty if the gas–particle interactions on filter substrates are not properly handled. Sampling artifacts depend on both meteorological conditions and the chemical mix of the atmosphere. Despite numerous of studies on the subject, very few have evaluated filter-based methods in the Asian environments. This paper reports the results of a comparison of the performances of two filter-based samplers, including a Thermo Anderson Chemical Speciation Monitor (RAAS) and a honeycomb denuder filter-pack system, a Micro Orifice Uniform Deposit Impactor (MOUDI) and a real-time ambient ion monitor (AIM, URG9000B) in measuring atmospheric concentrations of PM2.5 sulfate and nitrate. Field studies were conducted at an urban site in Jinan, Shandong province, during the winter of 2007 and at a rural site near Beijing in the summer of 2008. The AIM was first compared with the honeycomb denuder filter-pack system which was considered to have minimal sampling artifacts. After some modifications made to it, the AIM showed good performance for both sulfate and nitrate measurement at the two sites and was then used to evaluate other instruments. For the un-denuded RAAS, the extent of sampling artifacts for nitrate on quartz filters was negligible, while that on Teflon filters was also minimal at high nitrate concentrations (>10 μgm?3); however, loss through evaporation was significant (~75%) at low nitrate concentrations under hot summer conditions. The MOUDI using aluminum substrates suffered a significant loss of nitrate (50–70%) under summer conditions due to evaporation. Considering that the aluminum substrates are still being widely used to obtain size-resolved aerosol compositions because of their low cost and accurate mass weighed, caution should be taken about the potential significant under determination of semi-volatile components such as ammonium nitrate. 相似文献
96.
Ravi C. Palakodeti Eugene J. LeBoeuf James H. Clarke 《Journal of environmental management》2009,91(1):87-101
The groundwater/surface water interface (GWSWI) represents an important transition zone between groundwater and surface water environments that potentially changes the nature and flux of contaminants exchanged between the two systems. Identifying dominant and rate-limiting contaminant transformation processes is critically important for estimating contaminant fluxes and compositional changes across the GWSWI. A new, user-friendly, spreadsheet- and Visual Basic-based analytical screening tool that assists in evaluating the dominance of controlling kinetic processes across the GWSWI is presented. Based on contaminant properties, first-order processes that may play a significant role in solute transport/transformation are evaluated in terms of a ratio of process importance (Pi) that relates the process rate to the rate of fluid transfer. Besides possessing several useful compilations of contaminant and process data, the screening tool also includes 1-D analytical models that assist users in evaluating contaminant transport across the GWSWI. The tool currently applies to 29 organics and 10 inorganics of interest within the context of the GWSWI. Application of the new screening tool is demonstrated through an evaluation of natural attenuation at a site with trichloroethylene and 1,1,2,2-tetrachloroethane contaminated groundwater discharging into wetlands. 相似文献
97.
Ataria JM O'Halloran K Gooneratne R 《Environmental science and pollution research international》2007,14(4):256-265
Background Monitoring biological responses that are mediated via the aryl-hydrocarbon receptor (AhR) in animals exposed to environmental
contaminants can indicate both the presence to chemicals that act through this biochemical pathway and whether these chemicals
are bioavailable.
Objectives The use of an ex-situ method that incorporated biological responsiveness monitoring in mice for determining the presence of
‘biologically active’ hydrocarbons in contaminated soils was investigated.
Methods The use of C57BL/6 as a test organism was validated by determining hepatic and immune responsiveness to two polyaromatic hydrocarbons
(PAHs): 3,4 benz[a]pyrene (B[a]P) and 1,2 benz (a)anthracene (BA) administered via intraperitoneal (i.p.) injection. The responsiveness
of mice exposed to soils spiked with hydrocarbons or ex situ exposures to soil removed from two contaminated sites was also
investigated.
Results and Discussion Mice that were exposed to B[a]P via i.p. injections showed a 14-fold increase in liver microsomal ethoxyresorufin O-deethylase
(EROD) activity compared to the control group. In contrast EROD activity following BA exposure at the same level was not significantly
enhanced. Mouse immune response was significantly inhibited in a dose-dependent manner by i.p. injections of B[a]P. No significant
inhibition occurred with the same doses of BA. Following i.p. exposure, the retention of B[a]P in mouse carcasses was greater
than BA. Mice exposed to clean soils spiked with environmentally relevant concentrations of B[a]P and BA failed to show any
significantly different hepatic or immune responses. Carcass residue data indicated a limited uptake of PAH from the soil.
In contrast, EROD activity in mice exposed (ex situ) to hydrocarbon-contaminated soils removed from a fuel-loading depot and
decommissioned gas works was significantly enhanced (4- and 2-fold respectively). However, this increase in EROD activity
did not appear to correlate with either soil or carcass PAH concentrations.
Conclusions and Outlook These results support the assumption that B[a]P has a higher affinity for the aryl hydrocarbon receptor (AhR) compared to
BA. Soil parameters such as organic carbon content, structure and particle size distribution can modulate the bioavailability
of contaminants to biological receptors. These factors are implicated in the lack of responsiveness demonstrated in the spiked
soil experiments. However the responsiveness of EROD activity in mice exposed (ex situ) to soil contaminated with complex
mixtures of hydrocarbon compounds confirms the potential usefulness of this model to determine the presence of ‘biologically
active’ compounds in aged soils removed from contaminated sites. 相似文献
98.
Assessment of toxicity of heavy metal contaminated soils by the toxicity characteristic leaching procedure 总被引:5,自引:0,他引:5
The concentrations of Cu, Zn, Pb and Cd in soils near a lead–zinc mine located in Shangyu, Zhejiang Province, China, were determined and their toxicity was assessed using the toxicity characteristic leaching procedure (TCLP) developed by the United States Environmental Protection Agency. The TCLP method is a currently recognized international method for evaluation of heavy metal pollution in soils. The available levels of Cu, Zn, Pb and Cd were 8.2–36, 23–143, 6.4–1367 and 0.41–2.2 mg kg−1, respectively, while the international standards were 15, 25, 5 and 0.5 mg kg−1, respectively. Soils around the mine were more polluted with Zn and Pb, followed by Cd and Cu. Moreover, the levels of heavy metals in the soils extracted by TCLP indicated that extraction fluid 2 was more effective than extraction fluid 1 in extracting the heavy metals from the polluted soils and there was a positive correlation between fluids 1 and 2. Available heavy metal contents determined by TCLP were correlated with soil total heavy metal contents. 相似文献
99.
100.
Vikram Ravi Joseph K. Vaughan Michael P. Wolcott Brian K. Lamb 《Journal of the Air & Waste Management Association (1995)》2019,69(3):289-304
Using a WRF-SMOKE-CMAQ modeling framework, we investigate the impacts of smoke from prescribed fires on model performance, regional and loc al air quality, health impacts, and visibility in protected natural environments using three different prescribed fire emission scenarios: 100% fire, no fire, and 30% fire. The 30% fire case reflects a 70% reduction in fire activities due to harvesting of logging residues for use as a feedstock for a potential aviation biofuel supply chain. Overall model performance improves for several performance metrics when fire emissions are included, especially for organic carbon, irrespective of the model goals and criteria used. This effect on model performance is more pronounced for the rural and remote IMPROVE sites for organic carbon and total PM2.5. A reduction in prescribed fire emissions (30% fire case) results in significant improvement in air quality in areas in western Oregon, northern Idaho, and western Montana, where most prescribed fires occur. Prescribed burning contributes to visibility impairment, and a relatively large portion of protected class I areas will benefit from a reduced emission scenario. For the haziest 20% days, prescribed burning is an important source of visibility impairment, and approximately 50% of IMPROVE sites in the model domain show a significant improvement in visibility for the reduced fire case. Using BenMAP, a health impact assessment tool, we show that several hundred additional deaths, several thousand upper and lower respiratory symptom cases, several hundred bronchitis cases, and more than 35,000 workday losses can be attributed to prescribed fires, and these health impacts decrease by 25–30% when a 30% fire emission scenario is considered.
Implications: This study assesses the potential regional and local air quality, public health, and visibility impacts from prescribed burning activities, as well as benefits that can be achieved by a potential reduction in emissions for a scenario where biomass is harvested for conversion to biofuel. As prescribed burning activities become more frequent, they can be more detrimental for air quality and health. Forest residue-based biofuel industry can be source of cleaner fuel with co-benefits of improved air quality, reduction in health impacts, and improved visibility. 相似文献