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A pure culture using benzene as sole carbon and energy sources was isolated by screening procedure from gasoline contaminated soil. The analysis of the 16S rDNA gene sequence, morphological and physiological characteristics showed that the isolated strain was a member of genus Bacillus cereus. The biodegradation performance of benzene by B. cereus was evaluated, and the results showed that benzene could be e ciently biodegraded when the initial benzene concentration was below 150 mg/L. The metabolites of anaerobic nitrate-dependent benzene oxidation by strain B. cereus were identified as phenol and benzoate. The results of substrate interaction between binary combinations for benzene, phenol and benzoate showed that the simultaneous presence of benzene stimulated the degradation of benzoate, whereas the addition of benzene inhibited the degradation of phenol. Benzene degradation by B. cereus was enhanced by the addition of phenol and benzoate, the enhanced e ects were more pronounced at higher concentration. To our knowledge, this is the first report that the isolated bacterial culture of B. cereus can e ciently degraded benzene under nitrate reducing conditions.  相似文献
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赵畅  王文昭  徐期勇 《环境科学》2016,37(12):4720-4726
通过淬灭细菌的群体感应系统来抑制生物膜形成、防止膜生物污染的方法近年来受到广泛关注. 本实验从实际运行污水处理厂活性污泥中分离出5株具有群体感应淬灭功能的菌株,其中菌株HG10对信号分子N-乙酰高丝氨酸环内酯(C6-HSL)分解能力最强. 经16S rRNA基因序列比对,初步鉴定为蜡样芽孢杆菌(Bacillus cereus). 用海藻酸钠将菌株HG10进行包埋固定,以探究其在膜过滤系统中对膜污染防治的效果. 结果表明,经过8 d培养,添加细菌包埋珠(SA-HG10)的实验组B中膜通量为181.29 L·(m2·h)-1,未投加包埋珠的对照组A膜通量为110.64 L·(m2·h)-1,B组膜通量比A组高出63.86%;对微滤膜片上生物膜中EPS含量测定表明,实验组B中EPS多糖和蛋白质含量较对照组A分别减少了29%和48%,疏水性蛋白质含量的大量减少是造成膜污染减弱的主要原因;膜表面胞外聚合物(EPS)总含量减少了43%,表明投放SA-HG10细菌包埋珠对过滤膜片上生物膜形成具有明显抑制作用,改善了膜过滤性能.  相似文献
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对菌株YB3进行了16S rRNA基因序列进化分析,并分别以NH4Cl、NaNO2、NaNO3、尿素和蛋白胨为单一氮源,配制了5种低氮源浓度培养基,研究YB3在与养殖水体相近营养水平条件下的生长与氨氮去除特性.结果显示,菌株YB3属于蜡样芽孢杆菌(Bacullis cereus),在5种培养基中均能够生长,菌悬液(吸光度OD600为1.0)接种量为1.0%(v/v)时,OD600由0.010增长到0.100~0.117.在NH4Cl培养基中,YB3的氨氮去除速率为1.23 mg·L-1·d-1,去除率为93.5%.在尿素、蛋白胨等有机氮源培养基中,YB3将首先导致氨氮的积累,累积倍数分别为51.69和3.38,之后开始去除,去除速率为1.56和0.29 mg·L-1·d-1,去除率为93.7%和26.8%.结果也表明,提高YB3接种量至8.0%(v/v),可以使蛋白胨培养基氨氮累积倍数下降至2.02,去除速率提高至1.07 mg·L-1·d-1,去除率最终达到98.4%.NaNO2和NaNO3培养基中均未检测到氨氮,而NH4Cl、尿素和蛋白胨培养基中也未检测到NO2--N和NO3--N,表明YB3的硝化、亚硝化和反硝化作用均不强烈,去除氨氮的同时将不会造成NO2--N和NO3--N等的大量积累.本文为菌株YB3在养殖水体调控与净化中的应用研究提供了实验基础和理论支持.  相似文献
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