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Binbin Sheng Depeng Wang Xianrong Liu Guangxing Yang Wu Zeng Yiqing Yang Fangang Meng 《Frontiers of Environmental Science & Engineering》2020,14(6):93
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Molecular approaches for the detection and monitoring of microbial communities in bioaerosols: A review 总被引:3,自引:0,他引:3
Keunje Yoo Tae Kwon Lee Eun Joo Choi Jihoon Yang Sudheer Kumar Shukl Sang-il Hwang Joonhong Park 《环境科学学报(英文版)》2017,29(1):234-247
Bioaerosols significantly affect atmospheric processes while they undergo long-range vertical and horizontal transport and influence atmospheric chemistry and physics and climate change. Accumulating evidence suggests that exposure to bioaerosols may cause adverse health effects, including severe disease. Studies of bioaerosols have primarily focused on their chemical composition and largely neglected their biological composition and the negative effects of biological composition on ecosystems and human health. Here, current molecular methods for the identification, quantification, and distribution of bioaerosol agents are reviewed. Modern developments in environmental microbiology technology would be favorable in elucidation of microbial temporal and spatial distribution in the atmosphere at high resolution. In addition, these provide additional supports for growing evidence that microbial diversity or composition in the bioaerosol is an indispensable environmental aspect linking with public health. 相似文献
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Monika Nowrotek ukasz Ja owiecki Monika Harnisz Gra&zdot yna Anna P aza 《Frontiers of Environmental Science & Engineering》2019,13(3):40
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Jiaheng Zhao Bing Li Pin Lv Jiahui Hou Yong Qiu Xia Huang 《Frontiers of Environmental Science & Engineering》2022,16(3):35
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Zhang D.Cao M.Li Y.Wang S.Hao P.Feng Z. 《应用与环境生物学报》2018,(6):1301-1306
It is of great significance to ability to obtain new natural products with diverse activities through the study of soil microorganisms. However, less than 1% of the total soil microorganisms can be cultured under laboratory conditions, thus limiting the discovery of new compounds. Metagenomics, by which the genomic DNA of soil microorganisms can be extracted and expressed in heterologous hosts, provides a new approach for the functional study of soil microorganisms. Natural halides have good bioactivities, including antibacterial and antitumor activities. Halogenases play an important role in biosynthesis, and introducing bioactivities of halogenated compounds. To investigate the potential of halogenated compounds production from soil microorganisms, a soil metagenomic library was screened by PCR for clones harboring reduced flavin adenine dinucleotide (FADH2) - dependent halogenase genes. Sixty-five positive clones were identified from the library, and the amino acid sequences of halogenase genes within the positive clones were analyzed. Phylogenetic analyses revealed that more than 85% of these genes were separated from known halogenases to form new clades in the phylogenetic tree; moreover the soilderived halogenases showed high diversity. By further biosynthetic gene analysis of the positive clones, a new type I polyketide biosynthetic gene sequence was identified, which is probably related to the biosynthesis of the halogenated type I polyketide. In conclusion, novel and diverse halogenase genes were identified on sixty-five metagenomic clones using a sequence-driven metagenomic approach, laying a foundation for the further discovery of novel natural halides biosynthetic gene clusters and halides. © 2018 Science Press. All rights reserved. 相似文献
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