Effects of elevated atmospheric CO2 and tropospheric O3 on tree branch growth and implications for hydrologic budgeting

The forest hydrologic budget may be impacted by increasing CO₂ and tropospheric O₃. Efficient means to quantify such effects are beneficial. We hypothesized that changes in the balance of canopy interception, stem flow, and through-fall in the presence of elevated CO₂ and O₃ could be discerned using image analysis of leafless branches. We compared annual stem flow to the results of a computerized analysis of all branches from the 2002, 2004, and 2006 annual growth whorls of 97 ten-year-old trees from the Aspen Free-Air CO₂ and O₃ Enrichment (Aspen FACE) experiment in Rhinelander, WI. We found significant effects of elevated CO₂ and O₃ on some branch metrics, and that the branch metrics were useful for predicting stem flow from birch, but not aspen. The results of this study should contribute to development of techniques for efficient characterization of effects on the forest hydrologic budget of increasing CO₂ and tropospheric O₃.     

Analysis of a Farquhar-von Caemmerer-Berry leaf-level photosynthetic rate model for Populus tremuloides in the context of modeling and measurement limitations

The balance of mechanistic detail with mathematical simplicity contributes to the broad use of the Farquhar, von Caemmerer and Berry (FvCB) photosynthetic rate model. Here the FvCB model was coupled with a stomatal conductance model to form an [A,g_s] model, and parameterized for mature Populus tremuloides leaves under varying CO₂ and temperature levels. Data were selected to be within typical forest light, CO₂ and temperature ranges, reducing artifacts associated with data collected at extreme values. The error between model-predicted photosynthetic rate (A) and A data was measured in three ways and found to be up to three times greater for each of two independent data sets than for a base-line evaluation using parameterization data. The evaluation methods used here apply to comparisons of model validation results among data sets varying in number and distribution of data, as well as to performance comparisons of [A,g_s] models differing in internal-process components.     

Will photosynthetic capacity of aspen trees acclimate after long-term exposure to elevated CO2 and O3?

Photosynthetic acclimation under elevated carbon dioxide (CO₂) and/or ozone (O₃) has been the topic of discussion in many papers recently. We examined whether or not aspen plants grown under elevated CO₂ and/or O₃ will acclimate after 11 years of exposure at the Aspen Face site in Rhinelander, WI, USA. We studied diurnal patterns of instantaneous photosynthetic measurements as well as A/C_i measurements monthly during the 2004-2008 growing seasons. Our results suggest that the responses of two aspen clones differing in O₃ sensitivity showed no evidence of photosynthetic and stomatal acclimation under either elevated CO₂, O₃ or CO₂ + O₃. Both clones 42E and 271 did not show photosynthetic nor stomatal acclimation under elevated CO₂ and O₃ after a decade of exposure. We found that the degree of increase or decrease in the photosynthesis and stomatal conductance varied significantly from day to day and from one season to another.     

Growth and Foliar Nitrogen Status of Four Plant Species Exposed to Atmospheric Ammonia

A chamber study was conducted to evaluate the growth response and leaf nitrogen (N) status of four plant species exposed to continuous ammonia (NH₃) for 12 weeks (wk). This was intended to evaluate appropriate plant species that could be used to trap discharged NH₃ from the exhaust fans in poultry feeding operations before moving off-site. Two hundred and forty bare-root plants of four species (Juniperus virginiana (red cedar), Gleditsia triacanthos var. inermis (thornless honey locust), Populus sp. (hybrid poplar), and Phalaris arundinacea (reed canary grass) were transplanted into 4- or 8-L polyethylene pots and grown in four environmentally controlled chambers. Plants placed in two of the four chambers received continuous exposure to anhydrous NH₃ at 4 to 5 ppm while plants in another two chambers received no NH₃. In each of the four chambers, 2 to 4 plants per species received no fertilizer while the rest of the plants were fertilized with a 100 ppm solution containing 21% N, 7% phosphorus, and 7% potassium. The results showed that honey locust was the fastest-growing species. The superior growth of honey locust among all species was also supported by its total biomass, root, and root dry matter (DM) weights. For all species there was a trend for plants exposed to NH₃ to have greater leaf DM than their non-exposed counterparts at 6 (43.0 vs. 30.8%; P = 0.09) and 12 wk (47.9 vs. 36.6%; P = 0.07), and significantly greater (P ≤ 0.05) leaf N content at 6 (6.44 vs. 3.67%) and 12 wk (7.05 vs. 3.51%) when exposed to NH₃. Numerically greater leaf DM due to NH₃ exposure was also consistently measured in poplar at both sampling periods. Hybrid poplar, as well as honey locust and reed canary grass, deposited 1.5 to 2-fold greater N in their leaves than red cedar tissues as a result of NH₃ exposure compared to non-exposed plants. Regardless of the effect of NH₃ on foliar color and damage score of the plants, the increase of foliar N content (g 100 g^?1 of fresh foliage weight) after NH₃ exposure at 6 and 12 wk was 0.45 and 0.87 for grass,1.25 and 1.34 for locust, and 2.67 and 6.09 for poplar. However, only honey locust likely benefited from ambient NH₃ as indicated by its consistent leaf color quality and lower damage score, compared with other species that were adversely affected by atmospheric NH₃.     

A survey of antennal responses by five species of coniferophagous bark beetles (Coleoptera: Scolytidae) to bark volatiles of six species of angiosperm trees

Summary. Using Porapak Q traps, we collected the bark volatiles of six angiosperm trees native to British Columbia: black cottonwood, Populus trichocarpa Torr. & A. Gray (Salicaceae), trembling aspen, P. tremuloides Michx. (Salicaceae), paper birch, Betula papyrifera Marsh. (Betulaceae), bigleaf maple, Acer macrophyllum Pursh (Aceraceae), red alder, Alnus rubra Bong. (Betulaceae), and Sitka alder, A. viridis ssp. sinuata (Regel) á. L?ve & D. L?ve (Betulaceae). Utilising coupled gas chromatographic-electroantennographic detection analysis, the captured volatiles were assayed for antennal responses in five species of coniferophagous bark beetles (Coleoptera: Scolytidae), sympatric with most or all of the angiosperm trees: the Douglas-fir beetle, Dendroctonus pseudotsugae Hopkins, the mountain pine beetle, D. ponderosae Hopkins, the spruce beetle, D. rufipennis (Kirby), the western balsam bark beetle, Dryocoetes confusus Swaine, and the pine engraver, Ips pini (Say). The identities of 25 antennally-active compounds were determined by coupled gas chromatographic-mass spectroscopic analysis, and co-chromatographic comparisons with authentic chemicals. The compounds identified were: hexanal, (E)-2-hexenal, (Z)-3-hexen-1-ol, 1-hexanol, heptanal, α-pinene, frontalin, benzaldehyde, β-pinene, 2-hydroxycyclohexanone, 3-carene, limonene, β-phellandrene, benzyl alcohol, (E)-ocimene, salicylaldehyde, conophthorin, guaiacol, nonanal, methyl salicylate, 4-allylanisole, decanal, thymol methyl ether, (E)-nerolidol, and dendrolasin. A number of these compounds are known semiochemicals that are active in the behaviour of other organisms, including bark beetles, suggesting a high degree of semiochemical parsimony. Antennally-active compounds ranged from seven in A. viridis to 17 in P. trichocarpa. The fewest number of compounds (9) were detected by I. pini and the largest number (24) were detected by D. pseudotsugae. Six compounds excited the antennae of all five species of bark beetles. The large number of antennally-active compounds detected in common by numerous bark beetles and present in common in numerous nonhost trees supports the hypothesis of olfaction-based recognition and avoidance of nonhost angiosperm trees during the process of host selection by coniferophagous bark beetles. Received 13 December 1999; accepted 14 March 2000     

Climatic and stream-flow controls on tree growth in a Western montane riparian forest

Humans have severely impacted riparian ecosystems through water diversions, impoundments, and consumptive uses. Effective management of these important areas is becoming an increasingly high priority of land managers, particularly as municipal, industrial, and recreational demands for water increase. We examined radial tree growth of four riparian tree species (Pinus jeffreyi, Populus trichocarpa, Betula occidentalis, and Pinus monophylla) along Bishop Creek, California, and developed models relating basal area increment (BAI) and relative basal area increment (RBAI) to climatic and stream flow variables. Between years 1995–1999, univariate regression analysis with stream flow explained 29 to 61% of the variation in BAI and RBAI among all species except P. trichocarpa; growth by P. trichocarpa was not significantly related to stream flows over this period. Stepwise linear regression indicated that species responded differently to climatic variables, and models based on these variables explained between 33 to 86% of variation in BAI and RBAI during the decade of the 1990s. We examined branch growth of P. trichocarpa for sensitivity to differences in stream flow regimes and found that annual branch growth did not vary between a high- and low-flow site, but that annual branch growth was significantly higher in wet years with greater stream flows. Our results support the establishment of site-specific management goals by land managers that take into account all of the important tree species present in riparian ecosystems and their differential responses to altered hydrologic condition. Instream flow requirements for maintaining tree growth and vigor are only one of the species-specific responses that need to be evaluated, and these assessments should attempt to separate experimentally stream-flow (managed) controls from climatic (unmanaged) controls on growth.     

胡杨林的衰退原因与林地恢复策略

胡杨广布于地中海沿岸并呈带状东延至中国和蒙古人民共和国。目前,胡杨林已普遍呈不同程度退化,以塔里木盆地为例,垦伐等人为因素使林地总面积迅速减少。现场观测发现,一些林区缺乏胸径20cm以下的立木,而该地从1972年以来断绝洪水来源,显示洪水对幼苗补给可能存在相关,该地断绝洪水多年后,2002年重新出现洪泛过程,洪水退后出现大量的幼苗生长,从而证实洪水过程是胡杨幼林发生的必要条件。本文就此现象提出恢复部分林地的对策。     

塔里木河下游胡杨对输水的生态响应

对塔里木河下游不同离河距离成过熟胡杨和输水后更新胡杨胸径、地径、树高、冠幅、枯枝比以及优势胡杨百分比的调查表明:随离河距离增加(地下水埋深增加),胡杨(大树)枯枝比增加,优势胡杨百分比降低;胸径、树高、冠幅随离河距离增加,增减规律不明显。输水后萌蘖更新胡杨的树高、冠幅和地径均随离河距离增加而降低。以上调查指标确定胡杨输水后恢复等级,离河50~300 m胡杨整体恢复"优",离河300~500 m胡杨恢复"良",离河500~1 050m胡杨恢复"中",离河1 050~1 500 m胡杨对输水响应微弱,整体恢复"差"。胡杨具明显的随离河距离增加恢复等级降低的梯度响应特征,生态输水促进了河畔带胡杨的恢复,水是制约塔里木河下游胡杨生长与恢复的关键因子。     

胡杨内源保护酶系可以指征宏观生态行为效果吗--塔里木河下游生态输水效应案例研究

在宏观生态系统的恢复中,通常关注的层面至多达到个体水平,即只计测个体的存在和测定所关注的可视特征,而忽略了个体的生理反应,特别是在实施恢复生态工程时,大规模地重新构造生物生活资源结构,常常难以估测这种变化对生物的有效性,加之可视生态效果表达的滞后性和可测试的复杂性,常常造成效果遗漏.因此,增加微观(细胞)层面的反应指示指标,可捕捉细微的个体反应,更准确的指征生态效果在个体上实现反应的可能性,这对于丰富恢复生态学理论是具有重大意义的.本文以塔里木河下游生态恢复工程为载体,探索了以内源保护酶系活力变化为指征的个体对生态输水的响应,结果表明超氧歧化酶(SOD)、过氧化物酶(POD)和膜脂过氧化产物丙二醛(MDA)不仅对累积输水效果具有指示性,而且对短期水干扰具有敏感的指示性,相关关系也因受水而强化.     

Heterologous overexpression of Populus euphratica BAK1;1 gene enhanced resistance to Pst DC3000 in tobacco北大核心CSCD

BRI1-ASSOCIATED RECEPTORKINASE1(BAK1), a leucine-rich repeat (LRR) receptor protein kinase, plays a significant role in brassinosteroid (BR) signaling. Furthermore, it combines with other LRR-RLKs protein to initiate immune response in plants. The objective of this study was to (1) investigate the function of the Populus euphratica BAK1;1 gene in the resistance of transgenic tobacco to Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) and (2) discuss the regulation pathway of PeBAK1;1 involved in the resistance to plant pathogen. We cloned the cDNA sequence of the P. euphratica PeBAK1;1 gene, constructed the pBI121-35S::PeBAK1;1 over-expression vector, and then transformed it into wild-type tobacco by Agrobacterium-mediated transformation to obtain PeBA K 1;1-overexpressing transgenic tobacco plants. The bioinformatic analysis showed that the PeBAK1;1 protein contained all the structural features of the plant SERK family. The phylogenetic tree showed that PeBAK1;1 has the highest sequence homology with PtBAK1. The gene expression profile results indicated that the expression of PeBAK1;1 in the root was higher than that in the leaf and stem. The wild-type tobacco plants showed an obvious susceptibility to Pst DC3000, whereas the transgenic plants exhibited enhanced resistance to Pst DC3000. Compared with that of the wild-type (WT), the real-time PCR and quantitative real-time PCR analysis revealed that the expression of pathogenesis-related genes (including PR1, PR3, PR4, and PR5), BAK1-interacting receptor kinase 1 gene, and BONZAI1 gene was upregulated in 35S::PeBAK1;1 transgenic tobacco plants. In conclusion, the PeBAK1;1 gene plays a positive regulatory role in 35S::PeBAK1;1 transgenic tobacco against Pst DC3000, which can enhance the resistance of plants to pathogen. © 2018 Science Press. All rights reserved.