Detection of fetal HLA-DQα sequences in maternal blood: A gender-independent technique of fetal cell identification

The objective of this study was to detect fetal HLA-DQα gene sequences in maternal blood. HLA-DQα genotypes of 70 pregnant women and their partners were determined for type A1. We specifically sought couples where the father, but not the mother, had genotype A1. In 12 women, maternal blood samples were flow-sorted. Candidate fetal cells were isolated and amplified by using PCR primers specific for a paternal HLA-DQα A1 allele. Fetal HLA-DQα A1 genotype was predicted from sorted cells; amniocytes or cheek swabs were used for confirmation. Six of twelve sorted samples had amplification products indicating the presence of the HLA-DQα A1 allele; 6/12 did not. Prediction of the fetal genotype was 100 per cent correct, as determined by subsequent amplification of amniocytes or cheek swabs. We conclude that paternally inherited uniquely fetal HLA-DQα gene sequences can be identified in maternal blood. This system permits the identification of fetal cells independent of fetal gender, and has the potential for non-invasive prenatal diagnosis of paternally inherited conditions.     

平衡调温调湿方式中变流量冷冻水系统设计

通过对变流量冷冻水系统的分析指出，在运用平衡调温调湿方式的空调系统中，应用变流量冷冻水系统可以较大地节约能源和投资；并提出了实现这种应用的方法。     

BASE FLOW TRENDS IN URBANIZING WATERSHEDS OF THE DELAWARE RIVER BASIN1

Rapid land development is raising concern regarding the ability of urbanizing watersheds to sustain adequate base flow during periods of drought. Long term streamflow records from unregulated watersheds of the lower to middle Delaware River basin are examined to evaluate the impact of urbanization and imperviousness on base flow. Trends in annual base flow volumes, seven‐day low flows, and runoff ratios are determined for six urbanizing watersheds and four reference watersheds across three distinct physiographic regions. Hydrograph separation is used to determine annual base flow and stormflow volumes, and nonparametric trend tests are conducted on the resulting time series. Of the watersheds examined, the expected effects of declining base flow volumes and seven‐day low flows and increasing stormflows are seen in only one watershed that is approximately 20 percent impervious and has been subject to a net water export over the past 15 years. Both interbasin transfers and hydrologic mechanisms are invoked to explain these results. The results show that increases in impervious area may not result in measurable reductions in base flow at the watershed scale.     

四川青衣江上游若壁小沟泥石流及其防治对策

若壁小沟为四川青衣江上游左岸的一级支沟,该沟处于深切割高山峡谷区,地形坡度陡,构造发育,岩石破碎,加之较为丰富的降雨,使该沟具备泥石流暴发的条件。该沟如果暴发泥石流,将对某电站拟建厂房产生威胁。该沟泥石流具有成灾快、危害严重、松散固体物质以坡面崩塌补给为主、活动频率中等等特征。通过野外考察和取样分析,该沟泥石流的性质和类型为中频率—中~大规模—低~高粘度泥石质泥石流。分析预测了该沟泥石流在强烈地震条件下、近期出现不同频率暴雨情况下及人类活动影响下的发展趋势。在此基础上,提出了该沟泥石流减灾工程规划方案。     

Bacteria transport and deposition under unsaturated conditions: the role of the matrix grain size and the bacteria surface protein

Unsaturated (80% water saturated) packed column experiments were conducted to investigate the influence of grain size distribution and bacteria surface macromolecules on bacteria (Rhodococcus rhodochrous) transport and deposition mechanisms. Three sizes of silica sands were used in these transport experiments, and their median grain sizes were 607, 567, and 330 microm. The amount of retained bacteria increased with decreasing sand size, and most of the deposited bacteria were found adjacent to the column inlet. The deposition profiles were not consistent with predictions based on classical filtration theory. The experimental data could be accurately characterized using a mathematical model that accounted for first-order attachment, detachment, and time and depth-dependent straining processes. Visual observations of the bacteria deposition as well as mathematical modelling indicated that straining was the dominant mechanism of deposition in these sands (78-99.6% of the deposited bacteria), which may have been enhanced due to the tendency of this bacterium to form aggregates. An additional unsaturated experiment was conducted to better deduce the role of bacteria surface macromolecules on attachment and straining processes. In this case, the bacteria surface was treated using a proteolitic enzyme. This technique was assessed by examining the Fourier-transform infrared spectrum and hydrophobicity of untreated and enzyme treated cells. Both of these analytical procedures demonstrated that this enzymatic treatment removed the surface proteins and/or associated macromolecules. Transport and modelling studies conducted with the enzyme treated bacteria, revealed a decrease in attachment, but that straining was not significantly affected by this treatment.     

基于信息熵理论的泥石流沟谷危险度评价

泥石流沟谷是一个复杂的地貌系统,有其发生、发展和衰退的过程.文章从系统科学和地貌学的角度出发,运用了能综合表述地貌演化特征的因子-地貌系统信息熵对泥石流沟谷进行危险度评价,研究以昆明市东川城区后山深沟、石羊沟和尼姑拉沟为试点,对各泥石流沟的信息熵进行了计算.结果表明,深沟和石羊沟的地貌信息熵值分别为0.124和0.174,泥石流沟谷正由发育的中年末期向老年期演化;尼古拉沟的信息熵值为0.246,正处于沟谷发育的旺盛时期,计算结果与当地实际情况有较好的一致性.     

粘性泥石流运动模型的实验研究

粘性泥石流运动模型的研究因受实验条件的限制,还很不完善。国内外学者应用较多的５种模型都有一定的适用性,但也都存在不同的缺陷。本文应用粘性泥石流土样中的细颗粒和粗颗粒进行流变实验,不仅得到了粗颗粒在浆体中的沉降特性和泥石流体的流变特性,还研究了粘性泥石流运动模型及其参数。所提模型能很好地反映泥石流体的流变特性,较现有的５种模型能更好地描述粘性泥石流。     

电子产品野外环境下温循加速载荷谱编制方法

目的 将电子产品在野外环境下日变化波动与季节差异明显的温度载荷编制成温循载荷谱和转换为加速载荷谱。方法 通过四点雨流计数法提取原谱中的载荷循环信息,对提取的循环信息进行分布拟合、相关性检验等统计分析,进而构建循环均值与范围值的联合概率密度函数,再运用概率密度法,编制出8×8二维环境载荷谱。在二维载荷谱基础上,编制出温循载荷谱,使用针对电子部件参数修正的加速方程转化为加速载荷谱。结果 利用野外作业现场1个作业周期内的气温纪录,提供了一套编制温循载荷谱和转换加速载荷谱的合理化流程和解决方案。结论 该制谱方法可以利用原始环境谱中绝大部分有效信息,较好地还原电子部件野外作业阶段经历的温度变化过程,为电子产品的加速寿命试验和使用寿命预测奠定基础。     

基于嵌套模型的地下水侧向径流边界刻画方法研究——以湖北碾盘山冲积平原地下水数值模拟为例

侧向径流边界的刻画方法是地下水数值模拟中的关键问题。以湖北碾盘山汉江水利水电枢纽工程为例,基于有限单元法和嵌套模型的水量转换方法,分别建立包括完整水文地质单元的区域模型和仅包含汉水Ⅰ级阶地的平原区模型;通过区域模型的分区水均衡运算,计算平原区侧向径流边界的径流量,并以区域模型水均衡为框架,分别采用二类流量边界和一类水头边界刻画侧向径流边界,建立了平原区模型;深入分析区域模型和平原区模型间参数和水量的转换关系,并对比了两类边界条件刻画方法的优缺点和适用条件。结果表明:采用嵌套模型能够得到较为准确的地下水侧向径流量;在平原区模型中采用二类流量边界刻画侧向径流边界有利于提高平原区模型的精度和稳定性;边界条件除具有双重含义外还具有双重特征,在模型建立过程中应充分认识边界条件的位置特征和数量特征,并合理概化。