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Subcellular distribution and toxicity of cadmium in Potamogeton crispus L   总被引:2,自引:0,他引:2  
Xu Q  Min H  Cai S  Fu Y  Sha S  Xie K  Du K 《Chemosphere》2012,89(1):114-120
The submerged macrophyte Potamogeton crispus L. was subjected to varying doses of cadmium (0, 20, 40, 60 and 80 μM) for 7 d, and the plants were analyzed for subcellular distribution of Cd, accumulation of mineral nutrients, photosynthesis, oxidative stress, protein content, and ultrastructural distribution of calcium (Ca). Leaf fractionation by differential centrifugation indicated that 48-69% of Cd was accumulated in the cell wall. At all doses of Cd, the levels of Ca and B rose and the level of Mn fell; the levels of Fe, Mg, Zn, Cu, Mo, and P rose initially only to decline later. Exposure to Cd caused oxidative stress as evident by increased content of malondialdehyde and decreased contents of chlorophyll and protein. Photosynthetic efficiency, as indicated by the quenching of chlorophyll a fluorescence (Fv/Fm, Fo and Fm), decreased significantly, the extent of decrease being directly proportional to the concentration of Cd. Increased amounts of precipitates of calcium were noticed in the treated plants, located either outside the cell membrane or in chloroplasts, mitochondria, the nucleus, and the cytoplasm whereas control plants showed small deposits of the precipitates around surface of the vacuole membrane and in the intercellular space but rarely in the cytoplasm. Photosynthetic efficiency and oxidative stress could be used as indicators of physiological end-points in determining the extent of Cd phytotoxicity.  相似文献   
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通过盆栽试验,研究了分别投加1,3,9mmol/kg的EDTA、EGTA和柠檬酸(CA),新疆杨对干旱区污染土壤中Cd的吸收和富集特征.实验结果表明,投加螯合剂的处理土壤中水提态Cd的含量是对照的4.27~249.12倍,但杨树地上部的生物量和对照相比并未显著降低.投加螯合剂的处理显著提高了杨树叶部Cd的含量和积累量,其值分别是对照的1.05~2.90和1.42~2.77倍.综合来看,螯合剂的投加有效活化了土壤中的Cd和促进了Cd从杨树根部向其地上部的转移,3种螯合剂的作用效果表现为EGTA>EDTA>CA的顺序.  相似文献   
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还原增溶强化EGTA淋洗修复重金属污染土壤   总被引:1,自引:1,他引:1       下载免费PDF全文
采用还原剂调控土壤中重金属的活性,旨在强化其对乙二醇双(2-氨基乙基醚)四乙酸(EGTA)洗脱土壤重金属的洗脱效果.分析了EGTA投加量、还原剂类型、液固比、淋洗时间等对土壤重金属的影响,以及不同淋洗条件下土壤重金属浸出浓度与化学形态的差异,采用SEM-EDS和FTIR对处理前后土壤样品进行表征.结果表明,硫代硫酸钠(Na_2S_2O_3)可显著提高EGTA对重金属的洗脱率,当液固比为7.5∶1、淋洗时间为2 h、EGTA与Na_2S_2O_3投加量均为5 g·L~(-1)时,Cu、Zn、Pb和Cd洗脱率分别达85.0%、 60.7%、 88.6%和66.4%.与EGTA单独处理相比,EGTA/Na_2S_2O_3联合处理后, 4种重金属的浸出浓度均降低,Pb的降幅最大,土壤颗粒变小,团粒结构被破坏,O和Al含量减少,Si含量增加,降低了土壤矿物组分的吸附性;土壤有机质含量大幅降低,—OH振动显著减弱,配位能力较强的有机官能团减少,降低了土壤有机组分的吸附性.土壤中吸附性较强组分含量的降低是Na_2S_2O_3强化EGTA洗脱重金属的重要机制.  相似文献   
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