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进行了用纳氏试剂比色法直接测定海水中氨氮的试验。试验表明,可用酒石酸钾钠溶液—氢氧化钠溶液作为掩蔽剂排除海水中钙、镁离子对测定的干扰。着重对氢氧化钠溶液用量、显色剂用量和显色时间进行了试验,以用200g/L氢氧化钠溶液2.5mL,纳氏试剂1.5mL,显色25min为最佳测定条件,并对水样中的盐度影响作了考察。氨氮质量浓度在0.01mg/L—0.40mg/L范围内符合比耳定律,检出限为0.01mg/L;平行样相对偏差为9%,加标回收率在91%—108%之间;相对盐度为10—32的海水可以直接测定。 相似文献
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Out of three perceptual characteristics of the colour of any substance, the hue depends mostly on the spectral properties
of a substance, while the brightness and saturation depend also on the concentration of a substance and its thickness. Here,
we report that evident change of the hue of the colour (i.e., from green to red) is due to a change in concentration or the
thickness of a layer in some exceptional substances such as pumpkin seed oil or an aqueous solution of bromophenol blue. In
some regions of Central Europe, salad dressing is made preferably with the pumpkin seed oil, which has a strong characteristic
nut-like taste and remarkable properties of the colour: it appears red in a bottle, but green when served as a salad dressing.
The colour of the pumpkin seed oil was previously described as brownish yellow, dark green, dark green to red ochre or dark
reddish brown to light yellow green. We elucidated the physicochemical and physiological basis of such dichromatism by Beer-Lambert
law and by the characteristics of human colour perception. Our concept was corroborated by the outcome of calculations of
colour from spectral properties using colour matching functions. We found that dichromatism is observed if the absorption
spectrum of any substance has at least two local minima: one wide but shallow and one narrow but deep local minimum. 相似文献
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试验了碘的倍增反应用于微量碘的测定,用淀粉指示剂显色,最大吸收峰位于620mm,摩尔吸光系数可达1×105L·mol-1·Cm-1.0~6μg碘/10ml符合比耳定律,可用于盐湖水、矿泉水、油田水和食盐、海产品中微量碘的测定. 相似文献
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比色法测定水中微量三氯杀螨醇 总被引:1,自引:0,他引:1
使用比色法测定水中微量三氯杀螨醇的浓度。首先用正己烷萃取浓缩水中的三氯杀螨醇,比较了加入不同量的去离子水,不同浓度、不同量的NaOH,不同的反应显色温度和反应时间以及吡啶用量等对体系吸光度的影响。得出最佳的显色条件:0.2ml去离子水,0.1ml质量浓度为45%的NaOH溶液,与2.0ml吡啶混合,于100℃水浴中反应3.0min,反应液在4000r/min离心2.0min,取出反应液在530nm处测定吸光度值。此方法的标准工作曲线相关系数为0.9914,方法的绝对检出限为0.2μg三氯杀螨醇。样品加标回收率为83.7%~114.1%,相对标准偏差小于10%。 相似文献
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