首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   36篇
  免费   16篇
  国内免费   22篇
安全科学   2篇
环保管理   2篇
综合类   47篇
基础理论   9篇
污染及防治   9篇
评价与监测   5篇
  2022年   1篇
  2021年   3篇
  2020年   2篇
  2018年   1篇
  2016年   1篇
  2015年   2篇
  2014年   2篇
  2013年   8篇
  2012年   5篇
  2011年   1篇
  2010年   9篇
  2009年   7篇
  2008年   3篇
  2007年   3篇
  2006年   6篇
  2005年   6篇
  2004年   3篇
  2003年   1篇
  2002年   4篇
  2001年   2篇
  1997年   1篇
  1996年   1篇
  1995年   1篇
  1994年   1篇
排序方式: 共有74条查询结果,搜索用时 46 毫秒
1.
Hg2+不仅污染环境,还会引发多种疾病,危害人体健康.因此,实现对Hg2+的快速灵敏检测十分必要.传统的Hg2+检测方法存在一定的局限性,而基于T-Hg2+-T结构的汞离子生物传感器具有高特异性、高稳定性的优势,近些年得到广泛研究和应用.本文简要介绍了T-Hg2+-T结构的概念和特点,并从荧光型、比色型和电化学型3个方面综述了基于T-Hg2+-T结构的汞离子生物传感器的研究进展,对未来的发展前景进行了展望.  相似文献   
2.
When using stable enzyme genes from a thermophile to create a biosensor in Escherichia coli, it is vital that these genes be overexpressed in order to provide a sufficient supply of enzymes. In this study, overexpression of the NADH oxidase (Nox) gene from the thermophile Deinococcus geothermalis was successfully achieved with the aim of creating a stable biosensor active at room temperatures. To do so, modification of 10 nucleotides, GAAATTAACT, upstream of the start codon of the Nox gene was necessary.  相似文献   
3.
介绍了微生物传感器快速测定生化需氧量(BOD)的方法,通过对标准溶液、工业废水、地表水、污水处理厂废水的检测,分析其精密度和准确度,通过与BOD_5的比对,对方法的存在问题及适用性进行了探讨和研究,为该方法的发展及其在环境监测中的实际应用提供了技术依据。  相似文献   
4.
As zinc (Zn) is both an essential trace element and potential toxicant, the effects of Zn fixation in soil are of practical significance. Soil samples from four field sites amended with ZnSO4 were used to investigate ageing of soluble Zn under field conditions over a 2-year period. Lability of Zn measured using 65Zn radioisotope dilution showed a significant decrease over time and hence evidence of Zn fixation in three of the four soils. However, 0.01 M CaCl2 extractions and toxicity measurements using a genetically modified lux-marked bacterial biosensor did not indicate a decrease in soluble/bioavailable Zn over time. This was attributed to the strong regulatory effect of abiotic properties such as pH on these latter measurements. These results also showed that Zn ageing occurred immediately after Zn spiking, emphasising the need to incubate freshly spiked soils before ecotoxicity assessments.  相似文献   
5.
Biosorption of silver ions onto Bacillus cereus biomass was investigated. Overall kinetic experiments were performed for the determination of the necessary contact time for the attainment of equilibrium. It was found that the overall biosorption process was best described by pseudo second-order kinetic model. The crystals detected by scanning electron microscope and X-ray photoelectron spectroscopy suggested the precipitation was a possible mechanism of biosorption. The molecular genetics of silver resistance of B. cereus biomass was also detected and illustrated by a whole cell sensor tool.  相似文献   
6.
为了获得可用于快速检测环境中重金属污染的全细胞生物传感器,本研究将含有HA标签的萤火虫荧光素酶(LUC)基因重组到含有四膜虫金属硫蛋白MTT1启动子和微管蛋白终止子序列的载体pBX中,获得重组质粒pBX-LUC,用基因枪粒子轰击法将pBX-LUC转化入四膜虫细胞中,通过同源重组和巴龙霉素抗性筛选, LUC基因整合到四膜虫大核基因组MTT1 位点,获得含有LUC基因的细胞株B2086-LUC. B2086-LUC对重金属镉和汞的响应敏感,可检测的最低浓度为5~10ng/mL;对铜和锌的响应较弱,可检测的最低浓度为0.5~ 1mg/mL.因此,四膜虫B2086-LUC可作为环境中镉和汞污染快速检测的全细胞生物传感器.  相似文献   
7.
微生物传感器测定河豚毒素研究   总被引:2,自引:0,他引:2  
以地衣芽孢杆菌、假单胞菌和枯草芽孢杆菌为识别元件,采用夹层法固定化微生物膜与氧电极组成毒性微生物传感器,实验了3种不同菌株生物传感器对河豚毒素的响应能力,得出对河豚毒素敏感的微生物菌株为地衣芽孢杆菌.实验得出地衣芽孢杆菌传感器最佳工作条件为pH=7.76,温度35℃,底物GGA质量浓度为21.5 mg/L.在此条件下传感器对河豚毒素响应的标准曲线y=0.0025x+0.0122,线性范围为10~100 μg/mL,相关系数0.9776.  相似文献   
8.
A mercury biosensor was constructed by integrating biosensor genetic elements into E. coli JM109 chromosome in a single copy number, using the attP/attB recombination mechanism of λphage. The genetic elements used include a regulatory protein gene (merR) along with operator/promoter (O/P) derived from the mercury resistance operon from pDU1358 plasmid of Serratia marcescens. The expression of reporter gene gfp is also controlled by merR/O/P. Integration of the construct into the chromosome was done to increase the stability and precision of the biosensor. This biosensor could detect Hg(II) ions in the concentration range of 100-1700 nmol/L, and manifest the result as the expression of GFP. The GFP expression was significantly different (P ≤ 0.05) for each concentration of inducing Hg(II) ions in the detection range, which reduces the chances of misinterpretation of results. A model using regression method was also derived for the quantification of the concentration of Hg(II) in water samples.  相似文献   
9.
单室MFC型生物毒性传感器对重金属离子的检测研究   总被引:2,自引:0,他引:2       下载免费PDF全文
吴锋  刘志  周奔  周顺桂  饶力群  王跃强 《环境科学》2010,31(7):1596-1600
构建了单室微生物燃料电池(air-cathode microbial fuel cell,ACMFC)型生物毒性传感器,以含重金属离子(Cd2+、Cu2+)人工配水为检测对象,分析了有毒物质对检测仪的抑制率与有毒物质浓度的线性关系.结果表明,①单室MFC型生物毒性传感器结构简单,操作方便,灵敏度较高,可用于水体重金属离子(Cd2+、Cu2+)生物毒性的快速检测;②在实验条件下,该生物毒性传感器检测时间4h,清洗时间2~10min,恢复时间4h;③检测结果显示,实验室自配Cd2+、Cu2+及其混合液IC20值分别为0.6、0.8和0.25mg/L,有毒物质浓度与MFC产电量的抑制率呈显著正相关,相关系数分别为0.9960、0.9744和0.9907.  相似文献   
10.
When using stable enzyme genes from a thermophile to create a biosensor in Escherichia coli, it is vital that these genes be overexpressed in order to provide a sufficient supply of enzymes. In this study, overexpression of the NADH oxidase (Nox) gene from the thermophile Deinococcus geothermalis was successfully achieved with the aim of creating a stable biosensor active at room temperatures. To do so, modification of 10 nucleotides, GAAATTAACT, upstream of the start codon of the Nox gene was necessary.  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号