Basic proteins in serratia marcescens

The Science of Nature -     

Sustainable consumption in Brazil: Identification of preliminary requirements to guide product development and the definition of public policies

Sustainable product development is closely related to sustainable consumption. The understanding of consumers' purchase, use and discard behaviours may facilitate the identification of requirements to guide manufacturers in the development of sustainable goods and services. The aim of this paper is to investigate consumers' perception about factors that motivate or discourage the consumption of sustainable products to identify demands and convert them into requirements. South Brazilian green and traditional consumers were asked to complete an exploratory qualitative questionnaire. Their answers were organized and compared to identify differences and similarities between the demands of these two groups. Furthermore, demands were converted into requirements for packages, products, manufactures, stores planning and discard systems. The interpretation of factors that motivate or discourage the purchase of sustainable products given by interviewees led to the creation of a list of possible public policies or programmes, aiming to support sustainable consumption. The results demonstrate the necessity of further quantitative investigation between consumer groups, for validation purposes.     

Auswirkungen einer Umweltvertr?glichkeitsprüfung auf die Errichtung von Abfall-Entsorgungsanlagen

Prof. Dr. Derlef MARX arbeiter theoretisch und praktisch an Antworten auf die Frage, wie der Umweltschutz noch besser in die Aufgabenfelder vonRaumordnung undLandesplanung integriert und das schwierige Problem der Abfall-Entsorgung m?glichst umweltneutral gel?st werden kann. Dabei hat die Umweltvertr?glichkeitsprüfung trotz der zahlreichen rechtlichen und methodischen Problem, die mit ihr verbunden sind, entscheidende instrumentelle Bedeutung. Detief Marx ist Vorsitzender des Arbeitskreises Abfalivermeidung und Abfallwirtschaft der Akademie für Raumforschung und Landesplanung und Gesch?ftsführer der DEMA-Consult, München. Sein Büro arbeitet für das Umweltbundesamt an einem UVP-Praxistest für eine thermische Abfall-Entsorgungsanlage.     

Übermikroskopische Struktur von Ritzbahnen

The Science of Nature -     

Influence of Suspended Clay Minerals and Humic Matter on the Solid Phase Extraction Efficiency of Selected Pesticides from Water

The purpose of this investigation was to determine the influence of humic acids (HA) and Ca-montmorillonite (CaM) on the solid-phase extraction (SPE) efficiency of atrazine, alachlor and α-cypermethrin from water samples at various pH-values. The nature and intensity of binding of the studied pesticides to CaM were determined by X-ray diffraction analysis and termogravimetric analysis (TGA) test. The studied pesticides eluted from discs were analysed by thin-layer chromatography (TLC). The effects of CaM and humic acid were generally pH-dependent and acted independently in extraction efficiency influence. Lower recovery of pesticides was observed at higher pH values when CaM was ≥0.1 g and was attributed to greater dispersion of clay, increased surface area and subsequent adsorption. Concentrations of dissolved organic carbon (DOC) in humic acid had less effect on the extraction efficiency when water was at pH 8 compared to water at pH 2, which was probably due to greater nonpolar interactions of the pesticides to the charge-neutralized humic acid molecule.     

A Gis‐Based Approach to Watershed Classification for Nebraska Reservoirs1

Abstract: The U.S. Environmental Protection Agency is charged with establishing standards and criteria for assessing lake water quality. It is, however, increasingly evident that a single set of national water quality standards that do not take into account regional hydrogeologic and ecological differences will not be viable as lakes clearly have different inherent capacities to meet such standards. We demonstrate a GIS‐based watershed classification strategy for identifying groups of Nebraska reservoirs that have similar potential capacity to attain a certain level of water quality standard. A preliminary cluster analysis of 78 reservoirs was performed to determine the potential number of Nebraska reservoir groups. Subsequently, a Classification Trees method was used to refine number of classes, describe the structure of reservoir watershed classes, and to develop a predictive model that relates watershed conditions to reservoir classes. Results suggest that Nebraska reservoirs can be represented by nine classes and that soil organic matter content in the watershed is the most important single variable for segregating the reservoirs. The cross‐validation prediction error rate of the Classification Tree model was 26.3%. Because all geospatial data used in this work are available nationally, the method could be adopted throughout the U.S. Hence, this GIS‐based watershed classification approach could provide water resources managers an effective decision‐support tool in managing reservoir water quality.     

A new monoclonal antibody against vitellogenin from rainbow trout (Oncorhynchus mykiss).

Monoclonal antibodies were developed against vitellogenin (vtg) of rainbow trout. This protein is used as a biochemical response to the exposure with estrogenic compounds. Several mice were immunized with purified vtg and the spleen cells of these mice were fused with myeloma cells. The resulting hybridoma cells were screened with an enzyme immunoassay for the production of specific anti-vtg antibodies. Twelve positive cell lines were detected. The hybridoma cell line B8D8 was adjusted to serum free medium; it produced monoclonal antibodies with a high selectivity and sensitivity. A detection limit of 5 microg/l vtg was achieved with a competitive enzyme immunoassay applying a preincubation step and a streptavidin-biotin amplification system (ABC system). Cross-reactivity with vtg of other species was detected with roach (Rutilus rutilus), flounder (Platichthys flesus) and dab (Limanda limanda). The assay was applied to rainbow trout (Oncorhynchus mykiss), which were exposed for six months to defined concentrations of effluents (10%, 20%, 30% and 40%) and surface water. Increased vtg levels in male fish are correlated with the increasing levels of effluent. The effluent levels in the exposure experiments are relevant for effluent loadings of the Berlin waterways during the seasons of the year.     

Juvenile morphology: A clue to the origins of the most mysterious of mysticetes?

The origin of the pygmy right whale (Caperea marginata) has long been one of the most vexing conundrums of marine mammal evolution. The extremely disparate skeletal structure of Caperea and a patchy fossil record have left morphology and molecules at odds: whereas most morphological analyses ally Caperea with right whales (Balaenidae), most molecular studies instead suggest a close relationship with rorquals (Balaenopteridae) and grey whales (Eschrichtiidae). The morphological evidence supporting a Caperea-balaenid clade consists of several shared features of the skull and mandible, as traditionally observed in adult individuals. Here, we show that at least two of these features, the ascending process of the maxilla and the coronoid process, arise from substantially different precursors early during ontogeny and therefore likely do not represent genuine synapomorphies. Both of these juvenile morphologies have adult counterparts in the fossil record, thus indicating that the ontogenetic variation in the living species may be a genuine reflection of differing ancestral states. This new evidence contradicts previous morphological hypotheses on the origins of Caperea and may help to reconcile morphological and molecular evidence.