Diet of four species of deep-sea isopods (Crustacea: Malacostraca: Peracarida) in the South Atlantic and the Southern Ocean

The food of four species of asellote isopods (Crustacea, Malacostraca), Haploniscus rostratus, Haploniscus unicornis, Acanthocope galatheae and Betamorpha fusiformis, was evaluated by analysis of their gut contents. The isopods were sampled at several stations on the abyssal plains of Guinea Basin, Angola Basin and Cape Basin (southeast Atlantic), the Weddell Sea abyssal plain and the Antarctic continental slope during the DIVA and ANDEEP expeditions in 2000, 2001 and 2005. While all species had mineral particles in their guts and mucus material was the most frequent food item, the remaining gut contents differed among species. Betamorpha fusiformis fed mostly on phytodetritus, especially in the Southern Ocean basins and ingested along with it whole calcareous foraminifers. Acanthocope galatheae showed some differences in gut contents between basins, but in the Guinea Basin, the contents were to a large extent stercomata, i.e., waste pellets of soft-walled foraminifers, i.e., the Komokiaceae. Indications were that the haploniscids were feeding on detritus and agglutinating foraminifers (stercomata). This indicates spatial differences in food availability for this diverse group of deep-sea isopods and the importance of poorly known foraminiferal groups, like the Komokiaceae, as a food source in the deep sea.     

Toxic masking and synergistic modulation of the estrogenic activity of chemical mixtures in a yeast estrogen screen (YES)

Background, aim and scope

Estrogenic and non-estrogenic chemicals typically co-occur in the environment. Interference by non-estrogenic chemicals may confound the assessment of the actual estrogenic activity of complex environmental samples. The aim of the present study was to investigate whether, in which way and how seriously the estrogenic activity of single estrogens and the observed and predicted joint action of estrogenic mixtures is influenced by toxic masking and synergistic modulation caused by non-estrogenic chemical confounders.

Materials and methods

The yeast estrogen screen (YES) was adapted so that toxicity and estrogenicity could be quantified simultaneously in one experimental run. Mercury, two organic solvents (dimethyl sulfoxide (DMSO) and 2,4-dinitroaniline), a surfactant (LAS-12) and the antibiotic cycloheximide were selected as toxic but non-estrogenic test chemicals. The confounding impact of selected concentrations of these toxicants on the estrogenic activity of the hormone 17ß-estradiol was determined by co-incubation experiments. In a second step, the impact of toxic masking and synergistic modulation on the predictability of the joint action of 17ß-estradiol, estrone and estriol mixtures by concentration addition was analysed.

Results

Each of the non-estrogenic chemicals reduced the apparent estrogenicity of both single estrogens and their mixtures if applied at high, toxic concentrations. Besides this common pattern, a highly substance- and concentration-dependent impact of the non-estrogenic toxicants was observable. The activity of 17ß-estradiol was still reduced in the presence of only low or non-toxic concentrations of 2,4-dinitroaniline and cycloheximide, which was not the case for mercury and DMSO. A clear synergistic modulation, i.e. an enhanced estrogenic activity, was induced by the presence of slightly toxic concentrations of LAS-12. The joint estrogenic activity of the mixture of estrogens was affected by toxic masking and synergistic modulation in direct proportion to the single estrogens, which allowed for an adequate adaptation of concentration addition and thus unaffected predictability of the joint estrogenicity in the presence of non-estrogenic confounders.

Discussion

The modified YES proved to be a reliable system for the simultaneous quantification of yeast toxicity and estrogen receptor activation. Experimental results substantiate the available evidence for toxic masking as a relevant phenomenon in estrogenicity assessment of complex environmental samples. Synergistic modulation of estrogenic activity by non-estrogenic confounders might be of lower importance. The concept of concentration addition is discussed as a valuable tool for estrogenicity assessment of complex mixtures, with deviations of the measured joint estrogenicity from predictions indicating the need for refined analyses.

Conclusions

Two major challenges are to be considered simultaneously for a reliable analysis of the estrogenic activity of complex mixtures: the identification of known and suspected estrogenic compounds in the sample as well as the substance- and effect-level-dependent confounding impact of non-estrogenic toxicants.

Recommendations and perspectives

The application of screening assays such as the YES to complex mixtures should be accompanied by measures that safeguard against false negative results which may be caused by non-estrogenic but toxic confounders. Simultaneous assessments of estrogenicity and toxicity are generally advisable.     

Effects of the pharmaceuticals gemfibrozil and diclofenac on biomarker expression in the zebra mussel (Dreissena polymorpha) and their comparison with standardised toxicity tests

Pharmaceuticals, including the lipid regulator gemfibrozil and the non-steroidal anti-inflammatory drug diclofenac have been identified in waste water treatment plant effluents and receiving waters throughout the western world. The acute and chronic toxicity of these compounds was assessed for three freshwater species (Daphnia magna,Pseudokirchneriella subcapitata, Lemna minor) using standardised toxicity tests with toxicity found in the non-environmentally relevant mid mg L⁻¹ concentration range. For the acute endpoints (IC₅₀ and EC₅₀) gemfibrozil showed higher toxicity ranging from 29 to 59 mg L⁻¹ (diclofenac 47-67 mg L⁻¹), while diclofenac was more toxic for the chronic D. magna 21 d endpoints ranging from 10 to 56 mg L⁻¹ (gemfibrozil 32-100 mg L⁻¹). These results were compared with the expression of several biomarkers in the zebra mussel (Dreissena polymorpha) 24 and 96 h after exposure by injection to concentrations of 21 and 21,000 μg L⁻¹ corresponding to nominal concentrations of 1 and 1000 μg L⁻¹. Exposure to gemfibrozil and diclofenac at both concentrations significantly increased the level of lipid peroxidation, a biomarker of damage. At the elevated nominal concentration of 1000 μg L⁻¹ the biomarkers of defence glutathione transferase and metallothionein were significantly elevated for gemfibrozil and diclofenac respectively, as was DNA damage after 96 h exposure to gemfibrozil. No evidence of endocrine disruption was observed using the alkali-labile phosphate technique. Results from this suite of biomarkers indicate these compounds can cause significant stress at environmentally relevant concentrations acting primarily through oxidation pathways with significant destabilization of the lysosomal membrane and that biomarker expression is a more sensitive endpoint than standardised toxicity tests.     

Acclimatization patterns in tropical reptiles: uncoupling temperature and energetics

The physiological compensation of animals in changing environments through acclimatization has long been considered to be of minor importance in tropical ectotherms due to more stable climatic conditions compared to temperate regions. Contrasting this assumption are reports about a range of metabolic adjustments in tropical species, especially during the last two decades from field acclimatized animals. Metabolic rates are strongly linked to temperature in ectotherms but they also reflect energetic requirements and restrictions. We therefore postulate that the observed variety of acclimatization patterns in tropical reptiles results from an interaction of multiple influences, including food and water availability, rather than from thermal constraints alone. We present new data from two sympatric Malagasy lizards with contrasting acclimatization patterns and, complemented with an extensive literature search, discuss the variety of acclimatization patterns in tropical reptiles with regard to thermal and energetic influences. This broad consideration of constraints allows a rearrangement of apparently controversial patterns into a scheme of decreasing metabolic costs, including two new categories for selective and selective inverse acclimatization, where metabolic shifts are restricted to body temperatures below those preferred during activity.     

Farm-level assessment of CO2 and N2O emissions in Lower Saxony and comparison of implementation potentials for mitigation measures in Germany and England

Greenhouse gases (GHG) emissions from agricultural farming practice contribute significantly to European GHG inventories. For example, CO₂ is emitted when grassland is converted to cropland or when peatlands are drained and cultivated. N₂O emissions result from fertilization. Enabling farmers to reduce their GHG emissions requires sufficient information about its pressure–impact relations as well as incentives, such as regulations and funding, that support climate-friendly agricultural management. This paper discusses potentials to improve the supply of information on: farm-specific climate services or impacts, present policy incentives in Germany and England that support climate-friendly farm management and related adaptation requirements. Tools which have been developed for a farm environmental management software (to be added after review because of potential identification) are presented. These tools assess CO₂ emissions from grassland conversion to cropland and peatland cultivation, as well as N₂O emissions from nitrogen fertilization. As input data, the CO₂ tool requires a classification of soil types according to soil organic carbon storage. The input data based on soil profile samples was compared with reference data from the literature. The N₂O tool relies on farm data concerning fertilization. These tools were tested on three farms in order to determine their viability with respect to the availability of required data and the differentiation of results, which determines how well site-specific conservation measures can be identified. Assessing CO₂ retention function of grassland conservation to cropland on the test farms leads to spatially differentiated results (~100 to ~900 potentially mitigated t CO₂ ha^?1). Assessed N₂O emissions varied from 0.41 to 1.1 t CO₂eq. ha^?1 a^?1. The proposed methods support policies that promote a more differentiated funding of climate conservation measures. Conservation measures and areas can be selected so that they will have the greatest mitigation effects. However, even though present policy instruments in Germany and England, such as Cross Compliance and agri-environmental measures, have the potential to reduce agricultural GHG, they do not appear to guide measures effectively or site-specifically. In order to close this gap, agri-environmental measures with the potential to support climate protection should be spatially optimized. Additionally, the wetland restoration measures which are most effective in reducing GHG emissions should be included in funding schemes.     

Are “job hoppers” trapped in hedonic treadmills? Effects of career orientations on newcomers' attitude trajectories

Joining a new organization to change jobs is an influential event in an employee's career. Thus, inter-organizational job changes have sparked growing scholarly interest, especially in the temporal dynamics involved in detaching from organizations and adapting to new ones. While it is widely accepted that employees adapt differently to job changes, the influence of employees' career orientations on changes in job attitudes has not yet been considered. This is surprising given that a key difference between self-centered and organization-centered career orientations is a positive attitude toward job changes. Building on hedonic adaptation, we examined how career orientations influence changes in job satisfaction and turnover intention throughout a job change. We compared self-centered and organization-centered employees using random coefficient modeling on two longitudinal data sets with voluntary job changers. Our results illustrate that self-centered career orientations foster a stronger decline in job satisfaction with the new employer, as well as a larger increase in turnover intention, than organization-centered career orientations. In contrast, employees with organization-centered career orientations experienced an upward trend in job satisfaction toward the end of the first year. Our findings offer important implications for research on the determinants of job attitude trajectories when individuals join a new organization.     

Prospecting at conspecific nests and exploration in a novel environment are associated with reproductive success in the jackdaw

Consistent behavioural differences between individuals of the same population (“personality” variation) might arise if individuals follow different life-history strategies. Thus, it would be important to determine how personality variation relates to behaviours potentially associated with life-history strategies, such as those related to the use of information about the state of the environment. Little is, however, known about how personality is associated with information use and reproductive success. We tested whether wild social jackdaws, Corvus monedula, show consistent behavioural differences in their exploratory behaviour (in a novel environment in the lab and in their reaction towards a novel object in the wild) and prospecting behaviour (number of visits to conspecific nests). We furthermore examined whether these behavioural traits are linked with each other and predictors of reproductive success. Breeding jackdaws were consistent in their exploratory behaviour within, and in their prospecting behaviour between, years. Exploratory behaviour in the novel environment was correlated with the latency to approach a novel object in the wild but not with the frequency of prospecting at conspecific nests. Highly exploratory males and females and frequently prospecting males produced fewer fledglings than less exploratory individuals or less prospecting males, respectively. We discuss the importance of consistent individual differences in exploration and information sampling on individual fitness.     

澳大利亚中东部原始汇水保护区里的表吮贾第虫Giardia和隐小孢子虫Crptosporidium

我们首次证实在澳大利亚中东部没有都市和农业污染的原始汇水保护区的偏远小溪里存在着致病的原生动物表吮贾第虫(肠兰伯氏鞭毛虫)Giardia lamblia和隐小孢子虫Cryptosporidium parvum.     

Maintenance of coelomic fluid pH in sea urchins exposed to elevated CO2: the role of body cavity epithelia and stereom dissolution

Experimental ocean acidification leads to a shift in resource allocation and to an increased [HCO₃ ^?] within the perivisceral coelomic fluid (PCF) in the Baltic green sea urchin Strongylocentrotus droebachiensis. We investigated putative mechanisms of this pH compensation reaction by evaluating epithelial barrier function and the magnitude of skeleton (stereom) dissolution. In addition, we measured ossicle growth and skeletal stability. Ussing chamber measurements revealed that the intestine formed a barrier for HCO₃ ^? and was selective for cation diffusion. In contrast, the peritoneal epithelium was leaky and only formed a barrier for macromolecules. The ossicles of 6 week high CO₂-acclimatised sea urchins revealed minor carbonate dissolution, reduced growth but unchanged stability. On the other hand, spines dissolved more severely and were more fragile following acclimatisation to high CO₂. Our results indicate that epithelia lining the PCF space contribute to its acid–base regulation. The intestine prevents HCO₃ ^? diffusion and thus buffer leakage. In contrast, the leaky peritoneal epithelium allows buffer generation via carbonate dissolution from the surrounding skeletal ossicles. Long-term extracellular acid–base balance must be mediated by active processes, as sea urchins can maintain relatively high extracellular [HCO₃ ^?]. The intestinal epithelia are good candidate tissues for this active net import of HCO₃ ^? into the PCF. Spines appear to be more vulnerable to ocean acidification which might significantly impact resistance to predation pressure and thus influence fitness of this keystone species.     

PSII activity and pigment dynamics of Symbiodinium in two Indo-Pacific corals exposed to short-term high-light stress

This study examined the capacity for photoprotection and repair of photo-inactivated photosystem II in the same Symbiodinium clade associated with two coexisting coral species during high-light stress in order to test for the modulation of the symbiont’s photobiological response by the coral host. After 4 days exposure to in situ irradiance, symbionts of the bleaching-sensitive Pocillopora damicornis showed rapid synthesis of photoprotective pigments (by 44 %) and strongly enhanced rates of xanthophyll cycling (by 446 %) while being insufficient to prevent photoinhibition (sustained loss in F _v/F _m at night) and loss of symbionts after 4 days. By contrast, Pavona decussata showed no significant changes in F _v/F _m, symbiont density or xanthophyll cycling. Given the association with the same Symbiodinium clade in both coral species, our findings suggest that symbionts in the two species examined may experience different in hospite light conditions as a result of different biometric properties of the coral host.