Quantitative PCR analysis of house dust can reveal abnormal mold conditions

Indoor mold concentrations were measured in the dust of moldy homes (MH) and reference homes (RH) by quantitative PCR (QPCR) assays for 82 species or related groups of species (assay groups). About 70% of the species and groups were never or only rarely detected. The ratios (MH geometric mean : RH geometric mean) for 6 commonly detected species (Aspergillus ochraceus, A. penicillioides, A. unguis, A. versicolor, Eurotium group, and Cladosporium sphaerospermum) were >1 (Group I). Logistic regression analysis of the sum of the logs of the concentrations of Group I species resulted in a 95% probability for separating MH from RH. These results suggest that it may be possible to evaluate whether a home has an abnormal mold condition by quantifying a limited number of mold species in a dust sample. Also, four common species of Aspergillus were quantified by standard culturing procedures and their concentrations compared to QPCR results. Culturing underestimated the concentrations of these four species by 2 to 3 orders of magnitude compared to QPCR.     

Planning Tools for Developing Comprehensive Regional Monitoring Programs

We present a planning framework that structures thelarge amount of interrelated information involved indeveloping regional monitoring programs. By regionalwe mean programs that recognize the importance ofprocesses that occur on larger geographic scales andthat cut across more than one ecosystem component. Theframework helps visualize functional relationshipsamong qualitative ingredients such as public concernsand more concrete details such as individual dataelements. It portrays the flow of information amongthe components of a regional program and ties eachaspect of the program to management decision-makingneeds. This makes it easier, during the planningprocess, to ensure that monitoring will actuallyproduce useful information. Further, the frameworksnovel, graphically oriented, and readily accessibleformat makes key information equally available to thewide range of participants (regulators, scientists,managers, public) whose input and support arenecessary for program success.     

Prenatal application of fluorescent in situ hybridization (FISH) for identification of a mosaic Y-chromosome marker,IDIC(Yp)

An amniocentesis was performed at 13.3 weeks' gestation for advanced maternal age. A mosaic sex chromosome pattern was found: of 50 cells examined, 34 had a 45,X karyotype. In 14 cells with a modal number of 46, a recognizable Y was substituted by a small non-fluorescent marker. C-banding identified the marker as an isodicentric in 12 cells. In two cells, the non-fluorescent marker appeared to be monocentric and looked like a non-fluorescent del (Yq), but could have been an isodicentric Y with inactivation of one of the centromeres. Two cells with a modal number of 47 showed two copies of the monocentric marker. Fluorescent in situ hybridization with an alpha satellite Y-specific centromeric probe confirmed the Y-chromosome origin of the markers and allowed for more accurate prenatal diagnostic information.     

Mitigating urban sprawl effects: a collaborative tree and shade intervention in Phoenix,Arizona, USA

Communities in Phoenix are confronted with numerous challenges that adversely affect human health and safety, with disproportionate impacts on low-income communities. While some challenges are being addressed at the city level, new alliances at the neighbourhood level are initiating community development programmes and projects. This article reports on an intervention study carried out in collaboration with community representatives, city staff, and non-profit organisations to mitigate adverse effects of urban sprawl in the Sky Harbour Neighbourhood in Phoenix. Participatory research was conducted to design and test a tree and shade intervention. Challenges associated with navigating community desires and broader principles of sustainable development are discussed. The study offers a replicable and adaptable intervention research design aimed at empowering communities to meet urban challenges.     

Vitalizing effects of being outdoors and in nature

Five studies utilizing survey, experimental, and diary methods assessed the effects of being outdoors on subjective vitality. In Study 1, we used a vignette method to examine whether being outdoors was associated with vitality, above and beyond the influences of physical activity and social interactions. Study 2 explored the effects of being outdoors on vitality through an experimental design contrasting indoor and outdoor walks. In Study 3, participants were exposed to photographic scenes of either nature or buildings. Results showed that only the nature scenes enhanced subjective vitality. Studies 4 and 5 used a diary methodology to examine within-person variations in subjective energy as a function of being outdoors, again controlling for physical and social activity. Being outdoors was associated with greater vitality, a relation that was mediated by the presence of natural elements. Limitations of these studies are discussed, as well as their implications for research on energy and vitalization.     

Non-mosaic trisomy 20 presenting at 21 weeks' gestation as a thoraco-abdominal mass

Non-mosaic trisomy 20 is rare in fetuses surviving beyond the first trimester. We report a case of a fetus with non-mosaic trisomy 20 in amniotic fluid cultures obtained during the prenatal evaluation of an unusual thoraco-abdominal mass which was found at autopsy to be pulmonary sequestration. Gross inspection and autopsy of the fetus revealed multiple anomalies. Copyright © 2001 John Wiley & Sons, Ltd.     

Temporal persistence of biological patch structure in an abyssal benthic community

An analysis of living and dead components of a population of deep-sea agglutinated Foraminifera reveals that both segments of the fauna are dispersed nonrandomly on a scale of centimeters. Significant positive correlations between the distribution of living and dead individuals in several species imply that small-scale patch structure in this environment may persist for more than one generation. Mechanisms maintaining such structure may include habitat selection and reproductive patterns and suggest that dispersion pattern is the result of an active interaction between amimals and a heterogeneous physical and biotic environment.     

Public health aspects of volcanic hazards; Evaluation and prevention of excessive morbidity and mortality due to natural disasters

This is a summary of the proceedings of sessions on Volcanic Hazards at the First International Symposium on Public Health in Asia and the Pacific Basin, held 3–11th March 1983, in Honolulu, Hawaii. The Symposium was sponsored by the Association of Schools of Public Health, the U.S. Public Health Service and the Centers for Disease Control (CDC), the World Health Organization (WHO), the South Pacific Commission, and the East–West Center of the University of Hawaii. The full proceedings are being prepared for publication. The Symposium was supported in part by: The Pan American Health Organization and The Amoco Foundation.