Phytotoxicity to and uptake of flumequine used in intensive aquaculture on the aquatic weed, Lythrum salicaria L

Phytotoxicity of Flumequine on the aquatic weed Lythrum salicaria L. was determined by two laboratory models: a single concentration test, by which the effects of 100 mg l-1 were evaluated after 10, 20, 30 days and a multiple concentration test, by which the effects of 5000-1000-500-100-50 micrograms l-1 were evaluated after 35-day exposure. 100 mg l-1 are highly toxic and significantly decrease the growth of plants; this effect increases with time. Concentrations between 5000 and 50 micrograms l-1 induced hormesis in plants, by significantly increasing mean number and dimension of leaves and secondary roots. The effect is the highest at 50 micrograms l-1 and decreases with increase in concentration. Both toxic effect and hormesis can be related to plant drug uptake, quite high, in the order of micrograms g-1. The ecological implication of Flumequine contamination in aquatic environments and the possible use of Lythrum salicaria for bioremediation and/or monitoring technique are discussed.     

Phytotoxicity to and uptake of enrofloxacin in crop plants

Phytotoxicity of enrofloxacin on crop plants Cucumis sativus, Lactuca sativa, Phaseolus vulgaris and Raphanus sativus was determined in a laboratory model: the effect of 50, 100 and 5000 microgl(-1) were evaluated after 30 days exposure by measuring post-germinative growth of primary root, hypocotyl, cotyledons and leaves. Concentrations between 50 and 5000 microgl(-1) induced both toxic effect and hormesis in plants, by significantly modifying both length of primary root, hypocotyl, cotyledons and the number/length of leaves. A toxic effect is induced by high concentration (5000 microgl(-1)), while hormesis occurs at low concentrations (50 and 100 microgl(-1)). A continuum between toxic effect and hormesis is found in the four plant species. Both toxic effect and hormesis can be related to an efficient plant drug uptake, in the order of microgg(-1). Plants are able to metabolize enrofloxacin into ciprofloxacin, as also happens in animals; Cucumis, Lactuca and Phaseolus biologically convert about one quarter of stored enrofloxacin. The ecological implication of enrofloxacin contamination in terrestrial environments is discussed.     

Encapsulation of Nerol Oil in Polycaprolactone Polymer and Stability Evaluation

Journal of Polymers and the Environment - The objective of this study was to obtain and characterize Nerol essential oil encapsulated in PCL. The miniemulsion and solvent evaporation method was...     

Using OpenMI and a Model MAP to Integrate WaterML2 and NetCDF Data Sources into Flood Modeling of Genoa,Italy

Extreme hydrometeorological events such as flash floods have caused considerable loss of life and damage to infrastructure over recent years. Flood events in the Mediterranean region between 1990 and 2006 caused over 4,500 fatalities and cost over €29 billion in damage, with Italy one of the worst affected countries. The Distributed Computing Infrastructure for Hydro‐Meteorology (DRIHM) project is a European initiative aiming at providing an open, fully integrated eScience environment for predicting, managing, and mitigating the risks related to such extreme weather phenomena. Incorporating both modeled and observational data sources, it enables seamless access to a set of computing resources with the objective of providing a collection of services for performing experiments with numerical models in meteorology, hydrology, and hydraulics. The purpose of this article is to demonstrate how this flexible modeling architecture has been constructed using a set of standards including the NetCDF and WaterML2 file formats, in‐memory coupling with OpenMI, controlled vocabularies such as CF Standard Names, ISO19139 metadata, and a Model MAP (Metadata, Adaptors, Portability) gateway concept for preparing numerical models for standardized use. Hydraulic results, including the impact to buildings and hazards to people, are given for the use cases of the severe and fatal flash floods, which occurred in Genoa, Italy in November 2011 and October 2014.     

Synthesis and Characterization of Lactic Acid Oligomers: Evaluation of Performance as Poly(Lactic Acid) Plasticizers

The use of fully bio-based and biodegradable materials for massive applications, such as food packaging, is an emerging tendency in polymer research. But the formulations proposed in this way should preserve or even increase the functional properties of conventional polymers, such as transparency, homogeneity, mechanical properties and low migration of their components to foodstuff. This is not always trivial, in particular when brittle biopolymers, such as poly(lactic acid) (PLA), are considered. In this work the formulation of innovative materials based on PLA modified with highly compatible plasticizers, i.e. oligomers of lactic acid (OLAs) is proposed. Three different synthesis conditions for OLAs were tested and the resulting additives were further blended with commercial PLA obtaining transparent and ductile materials, able for films manufacturing. These materials were tested in their structural, thermal and tensile properties and the best formulation among the three materials was selected. OLA with molar mass (M_n) around 1,000 Da is proposed as an innovative and fully compatible and biodegradable plasticizer for PLA, able to replace conventional plasticizers (phthalates, adipates or citrates) currently used for films manufacturing in food packaging applications.     

Heavy Metals and Radiochemical Analyses in the Sediments of the Rapallo Harbour

To assess the natural and anthropogenic radiocontamination, sediment samples were collected in the Tigullio Gulf (Ligurian Sea). the results presented here indicate a significant Cs-137 contamination (about 20 Bqkg^-1 dry weight) while concentrations of “primordial” natural radioisotopes were similar to those detected in other coastal areas. Gross sedimentation rates, determined through measurements of Be-7 concentrations, were at about 4-5 cm in the last 6 months. Natural and artificial radioactivity concentrations have been compared with data obtained in other Mediterranean areas. Heavy metals (Cu, Cr, Mn, Ni, Zn, Pb, Cd, Hg) concentrations were also determined inside the bay. Most elements displayed quite constant concentrations through the sediment core, except for copper which had showed highest concentrations in the top 16 cm of the sediment.     

From science to policy—a geostatistical approach to identifying potential areas for cetacean conservation: a case study of bottlenose dolphins in the Pelagos sanctuary (Mediterranean Sea)

Cetaceans are top-level predators that serve as sentinels of the health and status of lower trophic levels in the marine ecosystem. For this reason they attract significant attention in marine conservation planning and often have been used to promote designation of reserve areas in many countries (e.g., Ligurian Sea, Moray Firth, Hawaiian Islands, The Gully, Wadden Sea, Banks Peninsula, and Golfo San José). Many policies are designed to protect cetaceans. For example, the Habitat Directive requires member states to select, designate, and protect sites that support certain natural habitats or species, such as the bottlenose dolphin, as Special Areas of Conservation (SACs) that aim to create a network of protected areas across the European Union known as Natura 2000. The boundaries of protected areas for cetacean species must be defined for management purposes. In recent years, many techniques have been developed to define the distribution of cetaceans in relation to habitat preferences. Although these models can provide an understanding of the ecological processes that determine species distribution, their application requires prior knowledge of the variables that should be included in the model, the interactions among these variables, and their effects on species distribution. Thus, the lack of available data in understudied areas precludes the application of these types of models. As an alternative, we describe a geostatistical approach to identifying areas that potentially should be designated as marine protected areas for cetaceans. We illustrate the application of the kriging algorithm to the bottlenose dolphin population that resides in the northwestern Mediterranean Sea. The data derived from a 7-year survey were used. The encounter rate is the only variable required for this method, making it very easy to apply. The resulting georeferenced and high resolution map includes areas most visited by bottlenose dolphins, which are called core areas. Core areas are helpful for establishing the boundaries of marine reserves for the protection of the species. The approach described herein is accurate, precise, unbiased, replicable to all highly mobile species and easy to understand by both researchers and policy makers.     

Genetic diversity and structure in Pelvetia fastigiata (Phaeophyta: Fucales): does a small effective neighborhood size explain fine-scale genetic structure?

We investigated the genetic diversity and genetic structure of southern California populations of the common intertidal fucoid seaweed Pelvetia fastigiata, (J. Ag.) De Toni by means of allozyme electrophoresis and estimates of genetic neighborhood area and size, which are the first for seaweeds. We predicted that P. fastigiata populations would exhibit relatively low genetic diversity and high genetic structure because the seaweed is monoecious and has limited dispersal of gametes and zygotes. This prediction was supported; genetic diversity indices were all low compared to other seaweeds studied, but high genetic structure was evident particularly within individual reefs. Geospatial statistical analyses (second-order analyses) revealed clustered distribution of glucose-6-phosphate isomerase (GPI) alleles at the scale of 1 to 6 m within three reefs. The rare alleles were distributed only at the landward third of the reefs. Genetic neighborhood area (2.3 m²) and size (133 individuals) were estimated from parent-offspring dispersal distributions of gametes and zygotes from attached thalli and also detached reproductive fragments, which contributed very little to the effective neighborhood size. The neighborhood size was in the small theoretical range in which genetic drift could be responsible for the within-reef genetic structure. This result was equivocal, because the stereotyped distribution of rare alleles on the tips of each reef was highly unlikely to be due to random events (6.9×10^-24). These results emphasize (1) the importance of allele mapping in addition to spatial statistics to elucidate genetic structure, and (2) that interpretation of genetic-structure statistics as evidence for gene flow can be complicated, even when supported with independent estimates of gene flow, if data are lacking on selection and sporadic migration events. The emerging pattern of low levels of polymorphisms in brown seaweeds will limit the use of Wright's F-statistics and will require alternative, more direct techniques for the analysis of mechanisms responsible for population genetic structure.