Heavy metal concentration in fish tissues inhabiting waters of “Buško Blato” reservoar (Bosnia and Herzegovina)

Heavy metals concentration (mercury, lead, cadmium, arsenic, copper, zinc and chromium) in tissues (muscles, liver, kidney and gonads) of Dalmatian barbelgudgeon, the nase, the souffie and brown trout, inhabiting waters of Busko Blato reservoir in Bosnia and Herzegovina, has been determined by atomic absorption spectrophotometry. The meat of the tested fish sorts does not contain elevated concentration of most analyzed heavy metals with exception of lead (higher than MAC in Italy, Germany and Denmark) and mercury (in muscles of brown trout higher than MAC in most countries). The lowest level of all heavy metals is always detected in gonads, with higher values in fry compared to milt for copper, zinc, chromium and arsenic. The highest copper concentration is observed in the liver from the souffie which is suggested as a suitable biomonitor for copper intoxication. In muscles of all fish sorts, lead was always present in much higher concentration than cadmium, while in kidneys of most fish sorts, lead and cadmium concentrations were similar. We showed that bioaccumulation of some heavy metals in the fish sorts analyzed is tissue and sex dependent. Also, we concluded that the small water exchange in reversible shallow reservoir does not induce elevated concentration of heavy metals in fish tissues inhabiting Busko Blato.     

Tracing the sources of gaseous components (<Superscript>222</Superscript>Rn,CO<Subscript>2</Subscript> and its carbon isotopes) in soil air under a cool-deciduous stand in Sapporo,Japan

Radon (²²²Rn) and carbon dioxide were monitored simultaneously in soil air under a cool-temperate deciduous stand on the campus of Hokkaido University, Sapporo, Japan. Both ²²²Rn and CO₂ concentrations in soil air varied with atmospheric (soil) temperature in three seasons, except for winter when the temperature in soil air remained constant at 2–3°C at depth of 80 cm. In winter, the gaseous components were influenced by low-pressure region passing through the observation site when the ground surface was covered with snow of ~1 m thickness. Carbon isotopic analyses of CO₂ suggested that CO₂ in soil air may result from mixing of atmospheric air and soil components of different origins, i.e. CO₂ from contemporary soil organic matter and old carbon from deeper source, to varying degrees, depending on seasonal meteorological and thus biological conditions.     

Chemical properties and biodegradability of waste paper mill sludges to be used for landfill covering.

Waste paper mill sludges originating from different effluent treatment and de-inking installations are complex mixtures of inorganic and organic particles. Due to their favourable physico-chemical, and microbiological characteristics, they may be conveniently reused for different purposes as such or after appropriate pretreatment. Sludges from the Slovenian paper industry were extensively tested for their chemical, stability and sealing properties. During the biodegradability tests, evolutions of greenhouse gases CO2, CH4 and H2S as well as the concentrations of released volatile organic acids, such as acetic, propionic, butyric, lactic and glycollic acids as the typical degradation products of organic materials, were measured. Some other important parameters of water leachates such as pH, redox potential, content of starch and leachable ions were also evaluated. The results indicate that most of them can be efficiently applied as alternative hydraulic barrier layers for landfill construction and covering instead of the more expensive clay due to their good geomechanical properties, chemical inertness and microbiological stability. Such replacement brings about considerable economical and ecological benefits as the waste is reprocessed as secondary raw material.     

Influence of nutritional status on some toxic and essential elements in the blood of women exposed to vehicular pollution in Ile-Ife, Nigeria

Blood levels of the elements Cu, Zn, Se, As, Cd, Hg, and Pb have been determined in 62 Nigerian women who were occupationally exposed to vehicular pollution. Mercury was determined using a direct mercury analyzer, while all the other elements were determined by an inductively coupled plasma mass spectrometer system. The mean values for all the toxic elements were all within the recommended desirable/tolerable limits, except for Se (0.44 μg/mL, compared with <0.2 μg/mL recommended by the WHO). More than 98 % of the subjects had blood selenium levels higher than this recommended limit. For As, Hg, and Pb, the corresponding figures of subjects with blood levels above the recommended limits were 4, 8, and 19.3 %, respectively. When the subjects were grouped according to their body mass indexes as normal, underweight, overweight, and obese, analysis of variance shows that mean blood levels of Cu, As, and, to some extent Hg were significantly different in the four nutritional status groups. Blood Hg level correlates significantly with blood As in all the groups, except in obese subjects. Also, a significant correlation between age and blood Hg was observed only in normal subjects and between age and blood Pb only in obese subjects. These results suggest that nutritional status could influence both elemental levels and the interactions between trace elements in the blood of female subjects. Nutrition is therefore a factor to consider in efforts to modify human susceptibility to toxic elements.     

Chemical composition and hygroscopic properties of size-segregated aerosol particles collected at the Adriatic coast of Slovenia

The chemical composition as well as the water uptake characteristics of aerosols was determined in size-segregated samples collected during November 2002 on the Slovenian coast. Major ions, water-soluble organic compounds (WSOC), short-chain carboxylic acids and trace elements were determined in the water-soluble fraction of the aerosol. Total aerosol black carbon (BC) was measured from filter samples. Our results showed that the origin of air masses is an important factor that controls the variation in the size distribution of the main components. Very high concentrations of WSOC as well as higher concentrations of BC were found under mostly continental influence. Besides the main ionic species (SO4(2-), NH4(+), K+) in the finest size fraction (0.17-0.53 microm), the concentration of NO3(-) was also high. The difference between the two different air mass origins is particularly expressed for Cl-, Na+, Mg2+ and Ca2+ determined in particles larger than 1.6 microm. As expected, a very good correlation was found between Na+ and Cl-. A good correlation was found between sea salt elements and elements of crustal origin (Na+, Cl-, Mg2+, Ca2+, Sr). A good relationship between typical anthropogenic tracers (K, V and Pb) was also observed. The mass growth factors, for all size fractions of aerosols collected under continental influence were very low (maximum 2.23 at 94%, 1.6-5.1 microm), while under marine influence the mass growth factors increased significantly with the particle size. At 97% humidity, the mass growth factors were 6.95 for the size fraction 0.53-1.6 microm and 9.78 for larger particles (1.6-5.1 microm).     

Nano-size radon short-lived progeny aerosols in Slovenian kindergartens in wintertime

Indoor air concentrations of radon and radon short-lived decay products, equilibrium factors, unattached fractions of radon short-lived decay products (f(un)), relative humidity, and temperature have been measured in 29 rooms of 13 Slovenian kindergartens, with an emphasis on f(un) as a crucial parameter in dose assessment. Dose conversion factors, based on the measured f(un) values were compared to epidemiology-based value of 5 mSv WLM(-1).     

Determination of sulfur oxides formed during the S(IV) oxidation in the presence of iron

The reaction products (i.e., sulfate (SO4(2-)) and dithionate (S2O6(2-))) of S(IV) oxidation in the presence of iron(III) under different experimental conditions were investigated. Ion-interaction chromatography was used for the separation of sulfate and dithionate using tetrabutylammonium hydroxide (TBAOH) as an ion-pair reagent. The chromatographic method was optimized by varying the composition of the mobile phase (i.e., concentration of TBAOH, acetonitrile and Na2CO3) and by varying the flow rate of the mobile phase. The method was successfully applied to the determination of dithionate formed during the S(IV) oxidation in the presence of Fe(III). In air-saturated solutions sulfate was observed as the only product, while in N2-saturated solutions dithionate was also determined, but it is the minor reaction product and represents about 4% of the total amount of oxidized HSO3- under the studied conditions.     

Trophic transfer and accumulation of mercury in ray species in coastal waters affected by historic mercury mining (Gulf of Trieste,northern Adriatic Sea)

Total mercury (Hg) and monomethylmercury (MMHg) were analysed in the gills, liver and muscle of four cartilaginous fish species (top predators), namely, the eagle ray (Myliobatis aquila), the bull ray (Pteromylaeus bovinus), the pelagic stingray (Dasyatis violacea) and the common stingray (Dasyatis pastinaca), collected in the Gulf of Trieste, one of the most Hg-polluted areas in the Mediterranean and worldwide due to past mining activity in Idrija (West Slovenia). The highest Hg and MMHg concentrations expressed on a dry weight (d.w.) basis were found in the muscle of the pelagic stingray (mean, 2.529 mg/kg; range, 1.179–4.398 mg/kg, d.w.), followed by the bull ray (mean, 1.582 mg/kg; range, 0.129–3.050 mg/kg d.w.) and the eagle ray (mean, 0.222 mg/kg; range, 0.070–0.467 mg/kg, d.w.). Only one specimen of the common stingray was analysed, with a mean value in the muscle of 1.596 mg/kg, d.w. Hg and MMHg contents in the bull ray were found to be positively correlated with species length and weight. The highest MMHg accumulation was found in muscle tissue. Hg and MMHg were also found in two embryos of a bull ray, indicating Hg transfer from the mother during pregnancy. The number of specimens and the size coverage of the bull rays allowed an assessment of Hg accumulation with age. It was shown that in bigger bull ray specimens, the high uptake of inorganic Hg in the liver and the slower MMHg increase in the muscle were most probably due to the demethylation of MMHg in the liver. The highest Hg and MMHg contents in all organs were found in the pelagic stingray, which first appeared in the northern Adriatic in 1999. High Hg and MMHg concentrations were also found in prey species such as the banded murex (Hexaplex trunculus), the principal prey of the eagle rays and bull rays, the anchovy (Engraulis encrasicholus) and the red bandfish (Cepola rubescens), which are preyed upon by the pelagic stingray, as well as in zooplankton and seawater. Based on previously published data, a tentative estimation of MMHg bioamagnification was established. The average increase in MMHg between seawater, including phytoplankton, and zooplankton in the Gulf was about 10⁴, and MMHg in anchovy was about 50-fold higher than in zooplankton. The bioaccumulation of MMHg between seawater and small pelagic fish (anchovy) amounted to 10⁶ and between water and the muscle of larger pelagic fish (pelagic stingray) to 10⁷. The MMHg increase between surface sediment and benthic invertebrates (murex) and between benthic invertebrates and small benthic fish was 10². Ultimately, the trophic transfer resulted in a 10³ accumulation of MMHg between water and muscle of larger benthic fish (bull ray, eagle ray, common stingray), suggesting lower bioaccumulation by benthic feeding species.     

Dose estimation and radon action level problems due to nanosize radon progeny aerosols in underground manganese ore mine

One of the essential parameters influencing of the dose conversion factor is the ratio of unattached short-lived radon progeny. This may differ from the value identified for indoor conditions when considering special workplaces such as mines. Inevitably, application of the dose conversion factors used in surface workplaces considerably reduces the reliability of dose estimation in the case of mines.This paper surveyed the concentration of radon and its short-lived radon progeny and identified the unattached fraction of short-lived radon progeny. As well equilibrium factor during the month of August was calculated simultaneously at two extraction faces in a manganese ore mine.During working hours the average radon concentrations were 220 Bq m⁻³ and 530 Bq m⁻³ at Faces 1 and 2; the average short-lived progeny concentration was 90 Bq m⁻³ and 190 Bq m⁻³, the average equilibrium factors were 0.46 and 0.36, and the average unattached fractions were 0.21 and 0.17, respectively. The calculated dose conversion factor was between 9 and 27 mSv WLM⁻¹, but higher values could also be possible.     

Fungal and enzymatic treatment of mature municipal landfill leachate

The aim of our study was to evaluate biotreatability of mature municipal landfill leachate by using white rot fungus and its extracellular enzymes. Leachates were collected in one active and one closed regional municipal landfill. Both chosen landfills were operating for many years and the leachates generated there were polluted by organic and inorganic compounds. The white rot fungus Dichomitus squalens was able to grow in the mature leachate from the closed landfill and as it utilizes present organic matter as a source of carbon, the results were showing 60% of DOC and COD removal and decreased toxicity to the bacterium Aliivibrio fischeri. On the other hand, growth of the fungus was inhibited in the presence of the leachate from the active landfill. However, when the leachate was introduced to a crude enzyme filtrate containing extracellular ligninolytic enzymes, removal levels of COD and DOC reached 61% and 44%, respectively. Furthermore, the treatment led to detoxification of the leachate to the bacterium Aliivibrio fischeri and to reduction of toxicity (42%) to the plant Sinapis alba. Fungal and enzymatic treatment seems to be a promising biological approach for treatment of mature landfill leachates and their application should be further investigated.