Technical and theoretical considerations in the HLA typing of amniotic fluid cells for prenatal diagnosis and paternity testing

HLA typing of amniotic fluid cells has been used for the prenatal diagnosis of the HLA linked diseases congenital adrenal hyperplasia (21-OH-deficiency (21-OH-def) type) and complement C4 deficiency and it has also been used for the prenatal de termination of paternity. There are, however, technical difficulties in this test associated with the weak expression of some B locus antigens on amniotic fluid cells, and theoretical difficulties related to associations between particular HLA antigens and the 21-OH-def allele. Since certain HLA-B locus antigens are found in significantly increased frequencies among patients with 21-OH-def, there is a relatively high incidence of HLA-B homozygosity among the patients and over 40 percent of the parents of these patients share one or more HLA-B locus antigens. Results of some prenatal HLA typing tests may thus be difficult to interpret, and supplementary tests should be used whenever possible. HLA typing of amniotic cells is, however, the only available procedure for prenatal diagnosis of C4 deficiency and it is the best available procedure for prenatal determination of paternity. A modification of our original procedure allows HLA typing to be performed with increased numbers of HLA typing sera, and sera with optimum reactivity for amniotic fluid cells have now been selected for the definition of most of the more commonly expressed HLA antigens. Although amniotic fluid cells do not express DR antigens, amniotic fluid cells can be typed for the HLA-linked marker glyoxalase I (GLO) and this may be the informative for prenatal diagnosis in some cases.     

Relictual Amphibians and Old-Growth Forests

Abstract: Terrestrial and aquatic herpetofauna were sampled by pitfall traps, time-constrained searches, and area-constrained searches (stream sites only) over a three-year period to examine the importance of forest age to amphibians and reptiles. Fifty-four terrestrial and 39 aquatic sites in Douglas-fir—dominated, mixed evergreen forests were located in southwestern Oregon and northwestern California Mean age of trees on sites ranged in age from 30 to 560 years. Thirty-one species of amphibians and reptiles were detected from the 93 localities. Only three species were found primarily on older forest sites: the Del Norte salamander (Plethodon elongatus), the Olympic salamander (Rhyacotriton olympicus), and the tailed frog (Ascaphus truei). Paleoeco-logic evidence indicates an historical association between these three amphibians and the extant elements of ancient primeval coniferous forests of the Pacific Northwest. The life histories and habitat requirements of these species suggest that these forms are scarce in younger forests because the microclimatic and microhabitat conditions they require generally exist only in older forests. The long-term viability of these species in northern California and southern Oregon may depend upon developing forestry practices that protect these mitical microclimates and microhabitats.     

Founding Lineages and Genic Variability in Plains Bison (Bison bison) from Badlands National Park, South Dakota

Abstract: Starch-gel electrophoresis was used to screen 101 bison from Badlands National Park, South Dakota, for variation at 24 genetic loci. The population was descended from founder groups of about 6 and 3 individuals, separated geographically for a minimum of 64 years. The purpose of this study was (1) to estimate levels of genic variability in this bison population, (2) to assess the extent to which descendents of the two founder groups differ genetically, and (3) to compare the genetic characteristics of the Badlands population with other bison populations. The Badlands herd was found to be polymorphic for only a single locus (MDH–1). Descendents of the founder groups were homogeneous with respect to allelic and genotypic frequencies at this locus. The MDH–1 polymorphism has not been observed in other bison populations, while several polymorphism reported in other bison populations were not detected in the Badlands herd. A mean heterozygosity of 0.012 was observed in the Badlands herd; this value is lower than that typically reported for mammals, though not as low as heterozygosities seen in other populations that have passed through severe bottlenecks in size. These results underscore the need for genetic data in planning breeding programs for species in captivity or managed in isolate reserves.     

Lands for Long-Term Research in Conservation Biology

Abstract: Success in conservation biology depends upon a synergistic combination of short-term tactics and long-term strategies. Although the former are often necessary to forestall immediate habitat losses, the latter provide the critical framework of understanding needed to develop effective short-term priorities. Although many conservation biologists now emphasize short-term tactics, prudence dictates the concomitant establishment of long-term research projects aimed at answering fundamental ecological questions.
One deterrent to amassing such long-term data bases, aside from time and funding, is a lack of suitable sites where such studies can be conducted on a large scale. We describe two established major land-holding networks in the United States that could serve as appropriate places to develop long-term studies: the National Science Foundation's Long-Term Ecological Research (LTER) sites, and the US. Department of Energy's National Environmental Research Parks (NERPs). Because they consist of established research facilities, both networks can provide conservation biologists with "low-cost" baseline information on ecological processes, as well as access to a number of representative terrestrial and aquatic habitats under more or less "controlled" conditions suitable for long-term studies. We recommend that conservation biologists explore the possibility of using these or similarly available sites in their research programs.     

Effects of Forest Fragmentation on Understory Hummingbirds in Amazonian Brazil

We sampled understory hummingbirds in Amazonian forest fragments from before isolation through nine years after isolation. We recorded 377 captures of eight species in five 1-ha fragments and four 10-ha fragments. The three species netted before isolation, Phaethornis superciliosus, Phaethornis bourcieri , and Thalurania furcata , were nearly equally abundant at that time. After isolation abundance of P. bourcieri and T. furcata did not change, but P. superciliosus became nearly twice as common. Five additional species that were netted only after isolation represented about 10% of the post-isolation sample. The species recorded only after isolation were forest species usually found above the levels of nets; fragments were not colonized by nonforest species. Use of fragments did not differ between 1- and 10-ha fragments. The landscape surrounding the fragments included active cattle pasture, abandoned pasture, and Cecropia -dominated second growth, but this variation had little effect on use of fragments by hummingbirds. The results suggest that these understory hummingbirds can persist in a matrix of fragments, secondary growth, and large forest patches. This response is much different than that of the insectivorous birds that dominate the understory bird community at the site, which are much more vulnerable to fragmentation.     

Infrastructure and Conservation Policy in Brazil

Abstract:  The development of transportation and energy infrastructure has been a major driver in the conversion of natural ecosystems in Brazil since the nineteenth century. Although this pattern is present in most countries, Brazil differs in the scale of opportunities that are still available to build its physical infrastructure while pursuing an ambitious conservation agenda. This advantage stems from the magnitude of intact ecosystems, a dynamic policy environment, and the increasing availability of biological and economic data needed to harmonize conservation with public works. Success depends on integrating conservation and infrastructure planning, rather than relying on the project based, largely ineffective environmental assessment process. Front-loading environmental mitigation and compensation will also help, as will improving public access to, and understanding of, information on the environmental and economic values at stake in major infrastructure decisions.     

Land Reform and Biodiversity Conservation in Brazil in the 1990s: Conflict and the Articulation of Mutual Interests

Abstract:  Land-reform and environmental movements, revitalized by the democratization of civil society in Brazil in the 1990s, found their objectives in conflict over forested parcels that settlers want for conversion to agriculture but that are important for wildlife conservation. In the Atlantic Forest, where 95% of the forest is gone, we reviewed three cases of Brazilian nongovernmental organization (NGOs) engagement with the land-reform movement with respect to forest remnants neighboring protected areas that have insufficient habitat for the long-term survival of unique endangered species. In the Pontal do Paranapanema (São Paulo), Poço das Antas (Rio de Janeiro), and southern Bahia, environmental NGOs have supported agricultural alternatives that improve livelihood options and provide incentives for habitat conservation planning. Where land-reform groups were better organized, technical cooperation on settlement agriculture permitted the exploration of mutual interests in conciliating the productive landscape with conservation objectives. Processes of regular consultation among NGOs, environmental agencies, and the private sector revealed that there was less zero-sum conflict over the same lands than commonly perceived. In both groups, technicians found forested lands less suitable for small-scale agriculture, and leaders took risks to justify and support claims to alternative existing agricultural lands. Based on the cases we examined, the construction of landscapes with both forest stewardship and poverty-reducing agrarian reform faces continued obstacles from contradictory agrarian and environmental sector policies and inadequate economic incentives for forest stewardship on private lands.     

Simple Spatial Modeling Tool for Prioritizing Prescribed Burning Activities at the Landscape Scale

Abstract:   Resources for prescribed fire are frequently insufficient to manage public lands for all conservation and resource management objectives, necessitating prioritization of the application of fire across the landscape within any given year. Defining tradeoffs when applying prescribed fire to large landscapes is problematic not only because of the complexity of weighing competing management objectives at the landscape scale, but also because of the difficult nature of independently applying need-to-burn criteria to large areas. We present a case study of a simple modeling process implemented at Eglin Air Force Base in the Florida Panhandle (U.S.A.) to prioritize the application of prescribed fire. In a workshop setting, managers and biologists identified key conservation criteria and landscape management objectives that drive the application of prescribed fire. Remote sensing and other spatial data were developed to directly or indirectly represent all these criteria. Using geographic information system software, managers and biologists weighted each criterion according to its relative contribution to overall burn prioritization, and individual values for the criterion were scored according to how they influence the need to burn. Subsequently, this process has been validated and modified through ecological monitoring. This modeling process has also been applied to the 77,400-ha Blackwater River State Forest, public land adjacent to Eglin Air Force Base, demonstrating its applicability to lands with varying management priorities. The advantages of this model-based approach for prioritizing prescribed fire include the reliance on accessible, inexpensive software, the development of spatially explicit management objectives, the ease of transferability, and clearly stated assumptions about management that may be tested and reviewed through monitoring and public comment.     

A drop in the bucket: when is a pay raise a pay raise?

Although merit pay continues to receive much theoretical and practical attention, little systematic effort is devoted to determining how large a pay raise must be before employees see it as a pay raise. This study uses psychophysical reasoning and techniques in a sample of 192 student ‘employees’ to establish the size of pay raise thresholds in a relatively controlled setting. Results indicate that, below about the 7 per cent level, increases in pay amounts are unlikely to evoke positive perceptual and attitudinal reactions among employees. Theoretical, methodological, and practical implications of these results are highlighted. © 1997 by John Wiley & Sons, Ltd.