Mycoremediation—a prospective environmental friendly technique of bioseparation and dewatering of domestic wastewater sludge

INTRODUCTION: Environmental safe and friendly management and disposal of wastewater sludge is a problem of every treatment plant throughout the world. Bioseparation and dewaterability of raw domestic wastewater sludge were evaluated for proper management and disposal by mycoremediation, i.e., using prior grown 2% (v/v) spore suspension of filamentous fungal (Mucor hiemalis Wehmer) broth inoculation, which were grown in 2% (w/v) solution of malt extract and wheat flour for 48-60 h in orbital shaker. DISCUSSION: Within 2-3 days of treatment application, encouraging results were achieved in total dry solids (TDS), total suspended solid (TSS), turbidity, chemical oxygen demand (COD), specific resistance to filtration (SRF), and pH due to fungal treatment in recognition of bioseparation and dewaterability of wastewater sludge compared to control. The significant reduction of TDS was remarked at fungal biomass (FB) in wheat flour (WF) treatment. The removal of TSS, turbidity, COD, and SRF were observed 96.0%, 99.4%, 92.6%, and 97.6%, respectively, in supernatant at 5 days by FB in WF. The SRF measuring the dewaterability was decreased with maximum (0.26 × 10(-12) mg/kg) equivalent to 95.5% at 2 days in FB in WF also. FB in WF broth is a potential, environmental friendly, comparatively low-cost biological technique which might play the significant role for bioremediation and bioseparation of domestic wastewater sludge. The present technique may bring a dynamic change in treatment of wastewater in future.     

Assessment of sewage sludge bioremediation at different hydraulic retention times using mixed fungal inoculation by liquid-state bioconversion

Sustainable, environmental friendly, and safe disposal of sewage treatment plant (STP) sludge is a global expectation. Bioremediation performance was examined at different hydraulic retention times (HRT) in 3–10 days and organic loading rates (OLR) at 0.66–7.81 g chemical oxygen demand (COD) per liter per day, with mixed filamentous fungal (Aspergillus niger and Penicillium corylophilum) inoculation by liquid-state bioconversion (LSB) technique as a continuous process in large-scale bioreactor. Encouraging results were monitored in treated sludge by LSB continuous process. The highest removal of total suspended solid (TSS), turbidity, and COD were achieved at 98, 99, and 93 %, respectively, at 10 days HRT compared to control. The minimum volatile suspended solid/suspended solid implies the quality of water, which was recorded 0.59 at 10 days and 0.72 at 3 days of HRT. In treated supernatant with 88 % protein removal at 10 days of HRT indicates a higher magnitude of purification of treated sludge. The specific resistance to filtration (SRF) quantifies the performance of dewaterability; it was recorded minimum 0.049 × 10¹² m kg^?1 at 10 days of HRT, which was equivalent to 97 % decrease of SRF. The lower OLR and higher HRT directly influenced the bioremediation and dewaterability of STP sludge in LSB process. The obtained findings imply encouraging message in continuing treatment of STP sludge, i.e., bioremediation of wastewater for environmental friendly disposal in near future.     

Deterioration of water quality of Surma river

Surma River is polluted day by day by human activities, poor structured sewerage and drainage system, discharging industrial and household wastes. The charas (natural channels) are responsible for surface runoff conveyance from its urban catchments to the receiving Surma River. Water samples have been collected from a part of Surma River along different points and analyzed for various water quality parameters during dry and monsoon periods. Effects of industrial wastes, municipal sewage, and agricultural runoff on river water quality have been investigated. The study was conducted within the Chattak to Sunamganj portion of Surma River, which is significant due to the presence of two major industries – a paper mill and a cement factory. The other significant feature is the conveyors that travel from India to Chattak. The river was found to be highly turbid in the monsoon season. But BOD and fecal coliform concentration was found higher in the dry season. The water was found slightly acidic. The mean values of parameters were Conductivity 84–805 μs; DO: dry-5.52 mg/l, monsoon-5.72 mg/l; BOD: dry-1mg/l, monsoon-0.878 mg/l; Total Solid: dry-149.4 mg/l, monsoon-145.7 mg/l. In this study, an effort has been taken to investigate the status of concentration of phosphate (PO⁻⁴) and ammonia–nitrogen (NH₄–N) at four entrance points of Malnichara to the city, Guali chara, Gaviar khal and Bolramer khal. Data has been collected from March–April and September–October of 2004. Concentrations have been measured using UV Spectrophotometer. Although the phosphate concentration has been found within the limit set by DOE for fishing, irrigation and recreational purposes, however ammonia–nitrogen has been found to exceed the limit.     

Evidential role of municipal solid waste and liquid effluent on environment and public health

Cities in Bangladesh produce large amounts of solid waste (SW) through various human activities which severely pollutes our native environment. As a result, SW pollutes the three basic environmental elements (air, water, and soil) by increasing pathogenic microbial load, which might be hazardous to public health directly or indirectly. In this study, we conducted 30 samples (i.e., soil, water, and air) collected from areas where municipal solid wastes are dumped (Tangail Sadar Upazila, Bangladesh). All the samples were analyzed to assess bacteriological quality for presumptive viable and coliform count using different agar media. We performed serial dilution 10⁻³–10⁻¹⁰ times for soil and water samples, and the diluted samples were spread on Mac-Conkey agar and nutrient agar plates. For the air sample, the sterile media containing petri-dish was placed adjacent to the dumpsite of the municipal waste and kept for an hour. Then all the samples were incubated at 37°C overnight for total viable count (TVC) and total coliform count (TCC). Biochemical tests and PCR were performed for the identification of these microorganisms. The antibiogram study was performed to reveal their (identified bacteria) susceptibility against clinically used antibiotics according to the standard disk diffusion technique. The highest bacterial loads were found in the air: TVC 3.273 × 10³ and TCC 1.059 × 10³ CFU/plate; tube-well water: TVC 8.609 × 10³, and TCC 8.317 × 10³ CFU/mL; in surface water: TVC 6.24 × 10¹³ CFU/mL and TCC 2.2 × 10¹² CFU/mL; in soil: TVC 2.88 × 10¹¹ and TCC 1.02 × 10¹¹ CFU/g, respectively. Microbes from SW can be transmitted through air, dust particles, or flies, and here we found an average of 1120 microbes spread over 63.61 cm² area per hour. Eight bacterial isolates (Pseudomonas spp., Klebsiella spp., E. coli, Proteus spp., V. cholera, Salmonella spp., Shigella spp., and Vibrio spp.) were identified by the biochemical test. Among them, E. coli and Shigella spp. were further ensured by PCR targeting bfpA and ipaH genes. Antibiotic susceptibility test results showed that E. coli isolates were highly resistant to erythromycin (80%); Shigella spp. were resistant to nalidixic acid (90%), whereas Salmonella spp. was found resistant to kanamycin (90%). Vibrio spp. were also resistant to azithromycin (80%) and erythromycin (80%), which should be a great concern for us. A semi-structured survey revealed that 63% of respondents suffered from different clinical conditions (intestinal diseases) due to SW pollution. So, steps should be taken to improve the proper management and disposal of solid waste and liquid effluent to save our environment and public health.     

Needle stick and sharps injuries among health care workers in Gondar city, Ethiopia

Background

All employees in health care settings handling needles or other sharps are at risk for needle stick and sharps injuries (NSSIs). Health care workers in under developed countries are at greater risk of infection from blood borne pathogens because of the lack of safety devices and the high prevalence of these pathogens.

Objectives

The aim of this study was to assess the prevalence of NSSIs and associated factors among health care workers in government health institutions in Gondar city, Ethiopia.

Methods

Institution based cross sectional study was conducted from May-December, 2010. The study included 344 health care workers who were selected from the source population using simple random sampling technique. Data were collected through interviews using structured and pre-tested questionnaire and the collected data were entered and analyzed using SPSS version 16.0.

Results

The prevalence of NSSIs among health care workers in the preceding 12 months was 106 (30.8%), of which 58 (54.7%) was reported by females. Lack of training on occupational health and safety, working more than 48 h/week, dissatisfaction with work environment and work culture, greater than 10 years of work experience and having low and moderate perception of risk were found to be significantly associated with NSSIs.

Conclusion

The study found high prevalence of NSSIs among health care workers. Effective training, ongoing awareness on the risk of hazards, preventive measures such as engineering control, and post-exposure prophylaxis following NSSIs are essential to reduce the risk of such injuries.     

Solid-state bioconversion of organic wastes into compost as bio-organic fertilizer by screened fungal isolates and its performance in agronomic usage

Journal of Material Cycles and Waste Management - Rejuvenation of composting of organic wastes (OW) by application of useful potential microbes is an emerging science of solid waste management....     

Evaluation of fungal potentiality for bioconversion of domestic wastewater sludge

This study was undertaken to screen the filamentous fungi isolated from its relevant habitats (wastewater, sewage sludge and sludge cake) for the bioconversion of domestic wastewater sludge. A total of 35 fungal strains were tested against wastewater sludge (total suspended solids, TSS 1%-5% w/w) to evaluate its potentiality for enhancing the biodegradability and dewaterability using liquid state bioconversion(LSB) process. The strains were divided into five groups i. e. Penicillium, Aspergillus, Trichoderma, Basidiomycete and Miscellaneous, respectively. The strains WWZP1003, SCahmA103, SCahmT105 and PC-9 among their respective groups of Penicillium,Aspergillus, Trichoderma and Basidiomycete played potential roles in terms of separation (formation of pellets/flocs/filaments),biodegradation(removal of COD) and filtration(filterability) of treated domestic wastewater sludge. The Miscellaneous group was not considered due to its unsatisfactory results as compared to the other groups. The pH value was also influenced by the microbial treatment during fermentation process. The filterability of treated sludge was improved by fungal treatment, and lowest filtration time was recorded for the strain WWZP1003 and SCahmA103 of Penicillium and Aspergillus groups respectively compared with other strains.