COMPREHENSIVE INFORMATION RETRIEVAL IN THE FIELD OF WATER RESOURCES1

The ages-old and ever-growing need for communication and information retrieval among scientists has found expression in the development of indexes, abstracts, reviews and combinations or variations of such. A short review of such efforts is followed by a discussion of efforts along these lines in the new field of water resources science and technology. Such undertakings as the Information Retrieval System of the American Society of Civil Engineers and of the U.S. Water Resources Research Office are compared with that of the American Water Resources Association.     

The use of environmental metabolomics to determine glyphosate level of exposure in rapeseed (Brassica napus L.) seedlings

Metabolic profiling in plants can be used to differentiate between treatments and to search for biomarkers for exposure. A methodology for processing Ultra-High-Performance Liquid Chromatography-Diode-Array-Detection data is devised. This methodology includes a scheme for selecting informative wavelengths, baseline removal, retention time alignment, selection of relevant retention times, and principal component analysis (PCA). Plant crude extracts from rapeseed seedling exposed to sublethal concentrations of glyphosate are used as a study case. Through this approach, plants exposed to concentrations down to 5 μM could be distinguished from the controls. The compounds responsible for this differentiation were partially identified and were different from those specific for high exposure samples, which suggests that two different responses to glyphosate are elicited in rapeseed depending on the level of exposure. The PCA loadings indicate that a combination of other metabolites could be more sensitive than the response of shikimate to detect glyphosate exposure.