Distribution and dietary regulation of an associated facultative Rhizobiales-related bacterium in the omnivorous giant tropical ant,Paraponera clavata

We document a facultative Bartonella-like Rhizobiales bacterium in the giant tropical ant, Paraponera clavata. In a lowland tropical rainforest in Costa Rica, 59 colonies were assayed for the prevalence of the Bartonella-like bacterium (BLB), 14 of which were positive. We addressed three questions: First, how does the prevalence of BLB within colonies vary with environmental conditions? Second, how does diet affect the prevalence of BLB in P. clavata? Third, how does the distribution of BLB among colonies reflect ambient differences in food resources and foraging habits? A variety of environmental variables that may be predictive of the presence of BLB were measured, and diet manipulations were conducted to test whether the prevalence of BLB responded to supplemental carbohydrate or prey. The ambient frequency of BLB is much higher in young secondary forests, but is nearly absent from older secondary forests. The prevalence of BLB inside field colonies increased over the duration of a 2-week carbohydrate supplementation; however, water and prey supplementation did not alter the prevalence of BLB. The diets of the colonies located in young secondary forest, compared to other habitats, have a diet richer in carbohydrates and lower in prey. The abundance of carbohydrate, or the relative lack of N, in a colony’s diet influences the occurrence of the BLB microbe in P. clavata. As experimental diet manipulations can affect the facultative presence of an N-cycling microbe, a consistent diet shift in diet may facilitate the emergence of tighter symbioses.     

Framing the search for a theory of land use

ABSTRACT

Land system science and affiliated research linked to sustainability require improved understanding and theorization of land and its change as a social-ecological system (SES). The absence of a general land-use theory, anchored in the social subsystem but with explicit links to the environmental subsystem, hampers this effort. Drawing on land-use explanations, meta-analyses, and associated frameworks, we advance a broad framework structure of eight elements – aggregations of explanatory variables – with links to the biophysical subsystem, for systematic comparisons of extant explanations. Tests and models can be employed to identify which set of variables and their configurations provide robust explanations of across land uses, identifying the potential for theory development. The framework and its application are applicable to both top-down and bottom-up explanatory approaches employed in the social sciences. Links to the environmental subsystem invite future exploration of SES explanations that reach across the different dimensions of global change and sustainability science.     

To drink or grasp? How bullet ants (Paraponera clavata) differentiate between sugars and proteins in liquids

Flexibility in behavior can increase the likelihood that a forager may respond optimally in a fluctuating environment. Nevertheless, physiological or neuronal constraints may result in suboptimal responses to stimuli. We observed foraging workers of the giant tropical ant (also referred to as the “bullet ant”), Paraponera clavata, as they reacted to liquid solutions with varying concentrations of sugar and protein. We show that when protein/sucrose concentration is high, many bullet ants will often try to grasp at the droplet, rather than gather it by drinking. Because P. clavata actively hunt for prey, fixed action patterns and rapid responses to protein may be adaptively important, regardless of the medium in which it is presented. We conclude that, in P. clavata, food-handling decisions are made in response to the nutrient content of the food rather than the texture of the food. Further, we suggest that colonies that maintain a mixture of individuals with consistent fixed or flexible behavioral responses to food-handling decisions may be better adapted to fluctuating environmental conditions, and we propose future studies that could address this.     

Downstream Dissipation of Storm Flow Heat Pulses: A Case Study and its Landscape‐Level Implications

Storms in urban areas route heat and other pollutants from impervious surfaces, via drainage networks, into streams with well‐described negative consequences on physical structure and biological integrity. We used heat pulses associated with urban storms as a tracer for pavement‐derived stormwater inputs, providing a conservative estimate of the frequency with which these pollutants are transported into and through protected stream reaches. Our study was conducted within a 1.5‐km reach in Durham, North Carolina, whose headwaters begin in suburban stormwater pipes before flowing through 1 km of protected, 100‐year‐old forest. We recorded heat‐pulse magnitudes and distances travelled downstream, analyzing how they varied with storm and antecedent flow conditions. We found heat pulses >1°C traveled more than 1 km downstream of urban inputs in 11 storms over one year. This best‐case management scenario of a reach within a protected forest shows that urban impacts can travel far downstream of inputs. Air temperature and flow intensity controlled heat‐pulse magnitude, while heat‐pulse size, mean flow, and total precipitation controlled dissipation distance. As temperatures and sudden storms intensify with climate change, heat‐pulse magnitude and dissipation distance will likely increase. Streams in urbanized landscapes, such as Durham municipality, where 98.9% of streams are within 1 downstream km of stormwater outfalls, will be increasingly impacted by urban stormwaters.     

Identifying Linkages Between Land Use,Geomorphology, and Aquatic Habitat in a Mixed-Use Watershed

The potential impacts of land use on large woody debris (LWD) were examined in Sourdough Creek Watershed, a rapidly growing area encompassing Bozeman, Montana, USA. We identified six land classes within a 250 m buffer extending on either side of Sourdough Creek and assessed aquatic habitat and geomorphologic variables within each class. All LWD pieces were counted, and we examined 14 other variables, including undercut bank, sinuosity, and substrate composition. LWD numbers were generally low and ranged from 0 to 8.2 pieces per 50 m of stream. Linear regression showed that LWD increased with distance from headwaters, riparian forest width, and sinuosity in four of the six land classes. Statistically significant differences between land classes for many aquatic habitat and geomorphologic variables indicated the impacts of different land uses on stream structure. We also found that practices such as active wood removal played a key role in LWD abundance. This finding suggests that managers should prioritize public education and outreach concerning the importance of in-stream wood, especially in mixed-use watersheds where wood is removed for either aesthetic reasons or to prevent stream flooding.