Monitoring of Phenol in Wastewater Bioremediation by HPLC

Bioremediation emphasizes the detoxification and destruction of toxic substances by microorganisms. Wastewater obtained from an industrial concern was solvent extracted with methyl alcohol and dichloromethane and analysed by GC/MS. Besides phenol, a large variety of organic compounds were detected. Under controlled laboratory conditions, the wastewater was innoculated with a mixed culture of microorganisms specially selected for their abilities to degrade phenol. Samples were collected at regular intervals from the stirred tank bioreactor and analysed for phenol by reverse phase HPLC with a C18 column. Results shows that from an initial phenol concentration of 987 ppm, slightly more than 50% was destroyed within 163 hours. The dry weight of the microorganisms and the plate count (CFU/ml) shows a steady increase from 0.5238 gms to 0.5355 gms and from 1.1E+9 to 1.94E+13 respectively over the same period. This suggested that the phenol was consumed by the microorganisms as the sole carbon source.     

A case study for a cost-benefit-based,stepwise optimization of thermo-chemical WAS pre-treatment for anaerobic digestion

Waste-activated sludge (WAS) may be considered a resource generated by wastewater treatment plants and used for biogas-generation but it requires pre-treatment (PT) for enhanced biogas-yields and reduced WAS disposal costs. To date, a number of studies on the optimization of such PT focused on improved biogas yields but neglected inferred energy and resource consumption. Here, we aimed to identify the most promising thermo-chemical PT-strategy in terms of net energy output and cost-efficiency by optimizing PT temperature and the amount and sort of the alkaline reagent used. We compared methane-potentials and disposal costs of untreated and treated WAS and conducted an annual cost-benefit calculation. We defined 70 °C and 0.04 M NaOH as ideal PT-conditions being both, low-energy demanding and efficient. Applying these conditions, enhanced biogas-yields and improved dewaterability led to reduced electricity and disposal costs of 22 and 27%, respectively, resulting in savings of approx. 28% of the yearly WAS-related expenditures of a wastewater treatment plant. Despite multiple benefits in running costs, the implementation of WAS-PT was not recommendable in the presented case study due to high investment costs.

     

Toxicological characterization of sediments from Baie des Anglais on the St. Lawrence Estuary.

The sediments of Baie des Anglais on the St. Lawrence Estuary have a history of environmental contamination, but no information exists relating to their toxicity. The purpose of the present study was to characterize three sites in and near Baie des Anglais, in terms of sediment toxicity and contaminants. Sites 1 and 2 within the Baie des Anglais are relatively close to local industry and municipal sewage discharge points, while Site 3 is outside the bay. Three microscale bioassays, Microtox, echinoderm fertilization and Toxi-ChromoPad, showed that sediments from Site 1 were the most toxic, followed by Site 2. Site 3 was non-toxic. While the solid phase Microtox test did indicate that Site 1 was most toxic, the absolute response was weak. Liver cytochrome P450 1A1 mRNA in American plaice (Hippoglossoides platessoides), captured at Site 1 in the bay was significantly induced compared to the P450 system of plaice captured at Sites 2 and 3. Hepatic metallothionein mRNA levels were not significantly different between plaice captured at all three sites. Sediment chemical analyses revealed a gradient in polycyclic aromatic hydrocarbons, polychlorinated biphenyls and dibenzofurans (PCDF) with the highest levels recorded at Site 1, about 10-fold less at Site 2 and 100-fold less at Site 3. Amongst the organochlorines the PCDF group were deemed the most important due to their prevalence and known toxicity. Heavy metal concentrations were low and representative of background levels for the St. Lawrence Estuary.     

Bioaccumulation and depuration of chromium in the selected organs and whole body tissues of freshwater fish Cirrhinus mrigala individually and in binary solutions with nickel

Contamination of aquatic ecosystems with heavy metals has been receiving increased worldwide attention due to their harmful e ects on human health and other organisms in the environment. Most of the studies dealing with toxic e ects of metals deal with single metal species, while the aquatic organisms are typically exposed to mixtures of metals. Hence, in order to provide data supporting the usefulness of freshwater fish as indicators of heavy metal pollution, it has been proposed in the present study to investigate the bioaccumulation and depuration of chromium in the selected organs of freshwater fingerlings Cirrhinus mrigala, individually and in binary solutions with nickel. The results show that the kidney is a target organ for chromium accumulation, which implies that it is also the “critical” organ for toxic symptoms. The results further show that accumulation of nickel in all the tissues of C. mrigala is higher than that of chromium. In addition, the metal accumulations of the binary mixtures of chromium and nickel are substantially higher than those of the individual metals, indicating synergistic interactions between the two metals. Theoretically the simplest explanation for an additive joint action of toxicants in a mixture is that they act in a qualitatively similar way. The observed data suggest that C. mrigala could be suitable monitoring organisms to study the bioavailability of water-bound metals in freshwater habitats.     

Bioaccumulation of lead and the influence of chelating agents in Catla catla fingerlings

Lead is a widespread element and one of the persistent and cumulative pollutants of the environment. The present study deals with the bioaccumulation of lead and the influence of chelating agents, meso 2,3-dimercaptosuccinic acid (DMSA), D-Penicillamine and CaNa₂EDTA in reducing the concentration of lead on the selected organs of Catla catla fingerlings for both acute and chronic exposures by using ICP-AES. It is inferred from the present findings that there was a correlation between environmental conditions and the heavy metal contents of the fish. The highest concentration of lead is found in kidney tissues and the lowest in muscle tissues. The accumulation pattern of lead in the selected organs of Catla catla is: kidney > liver > gill > brain > muscle. Also, it has been found that the treatment of chelating agents, DMSA, D-Penicillamine and CaNa₂EDTA reduces the concentration of lead significantly for both acute and chronic exposures. The results also show that DMSA is the most effective chelator of lead in reducing the body burden of C. catla fingerlings. The observed data further indicate that C. catla could be suitable for monitoring organisms to study the bioavailability of water-bound metals in freshwater habitats.     

Just Add Water and the Colorado River Still Reaches the Sea

A recent article in Environmental Management by All argued that flood flows in North America’s Colorado River do not reach the Gulf of California because they are captured and evaporated in Laguna Salada, a below sea-level lakebed near the mouth of the river. We refute this hypothesis by showing that (1) due to its limited area, the Laguna Salada could have evaporated less than 10% of the flood flows that have occurred since 1989; (2) low flow volumes preferentially flow to the Gulf rather than Laguna Salada; (3) All’s method for detecting water surface area in the Laguna Salada appears to be flawed because Landsat Thematic Mapper images of the lakebed show it to be dry when All’s analyses said it was flooded; (4) direct measurements of salinity at the mouth of the river and in the Upper Gulf of California during flood flows in 1993 and 1998 confirm that flood waters reach the sea; and (5) stable oxygen isotope signatures in clam shells and fish otoliths recorded the dilution of seawater with fresh water during the 1993 and 1998 flows. Furthermore, All’s conclusion that freshwater flows do not benefit the ecology of the marine zone is incorrect because the peer-reviewed literature shows that postlarval larval shrimp populations increase during floods, and the subsequent year’s shrimp harvest increases. Furthermore, freshwater flows increase the nursery area for Gulf corvina (Cynoscion othonopterus), an important commercial fish that requires estuarine habitats with salinities in the range of 26–38‰ during its natal stages. Although flood flows are now much diminished compared to the pre-dam era, they are still important to the remnant wetland and riparian habitats of the Colorado River delta and to organisms in the intertidal and marine zone. Only a small fraction of the flood flows are evaporated in Laguna Salada.     

Tamarix and Diorhabda Leaf Beetle Interactions: Implications for Tamarix Water Use and Riparian Habitat

Tamarix leaf beetles (Diorhabda carinulata) have been widely released on western United States rivers to control introduced shrubs in the genus Tamarix, with the goals of saving water through removal of an assumed high water‐use plant, and of improving habitat value by removing a competitor of native riparian trees. We review recent studies addressing three questions: (1) to what extent are Tamarix weakened or killed by recurrent cycles of defoliation; (2) can significant water salvage be expected from defoliation; and (3) what are the effects of defoliation on riparian ecology, particularly on avian habit? Defoliation has been patchy at many sites, and shrubs at some sites recover each year even after multiple years of defoliation. Tamarix evapotranspiration (ET) is much lower than originally assumed in estimates of potential water savings, and are the same or lower than possible replacement plants. There is concern that the endangered southwestern willow flycatcher (Empidonax trailli extimus) will be negatively affected by defoliation because the birds build nests early in the season when Tamarix is still green, but are still on their nests during the period of summer defoliation. Affected river systems will require continued monitoring and development of adaptive management practices to maintain or enhance riparian habitat values. Multiplatform remote sensing methods are playing an essential role in monitoring defoliation and rates of ET on affected river systems.     

Effect of acute concentration of zinc on the biochemical contents of brain of Labeo rohita: an FT-IR study

Heavy metal discharges to aquatic environment are of great concern due to their toxicity and accumulative behavior. Zinc is an essential trace element required for different physiological functions and plays important role in cellular metabolism. However, it becomes toxic when elevated concentrations are introduced into the environment. The aim of this work is to analyze zinc induced biochemical changes in the brain tissues of Labeo rohita fingerlings using Fourier Transformation Infrared Spectroscopy. Several important features have been observed in the zinc intoxicated brain tissues, namely, altered membrane lipid, altered protein profile and decreased glycogen content, indicating an alteration in the lipid and protein profiles leading to modification in membrane composition. Further, it is observed that the acute exposure to zinc causes some alteration in protein profile with a decrease in α-helix and an increase in random coil structures.     

Riparian vegetation dynamics and evapotranspiration in the riparian corridor in the delta of the Colorado River, Mexico

Like other great desert rivers, the Colorado River in the United States and Mexico is highly regulated to provide water for human use. No water is officially allotted to support the natural ecosystems in the delta of the river in Mexico. However, precipitation is inherently variable in this watershed, and from 1981-2004, 15% of the mean annual flow of the Lower Colorado River has entered the riparian corridor below the last diversion point for water in Mexico. These flows include flood releases from US dams and much smaller administrative spills released back to the river from irrigators in the US and Mexico. These flows have germinated new cohorts of native cottonwood and willow trees and have established an active aquatic ecosystem in the riparian corridor in Mexico. We used ground and remote-sensing methods to determine the composition and fractional cover of the vegetation in the riparian corridor, its annual water consumption, and the sources of water that support the ecosystem. The study covered the period 2000-2004, a flood year followed by 4 dry years. The riparian corridor occupies 30,000ha between flood control levees in Mexico. Annual evapotranspiration (ET), estimated by Moderate Resolution Imaging Spectrometer (MODIS) satellite imagery calibrated against moisture flux tower data, was about 1.1myr(-1) and was fairly constant throughout the study period despite a paucity of surface flows 2001-2004. Total ET averaged 3.4x10(8)m(3)yr(-1), about 15% of Colorado River water entering Mexico from the US Surface flows could have played only a small part in supporting these high ET losses. We conclude that the riparian ET is supported mainly by the shallow regional aquifer, derived from agricultural return flows, that approaches the surface in the riparian zone. Nevertheless, surface flows are important in germinating cohorts of native trees, in washing salts from the soil and aquifer, and in providing aquatic habitat, thereby enriching the habitat value of the riparian corridor for birds and other wildlife. Conservation and water management strategies to enhance the delta habitats are discussed in light of the findings.     

Phagocytic response of macrophages from the pronephros of American plaice (Hipoglossoides platessoides) exposed to contaminated sediments from Baie des Anglais, Quebec.

Sediments of Baie des Anglais on the St. Lawrence estuary have a history of environmental contamination, but little information exists regarding their toxicity. The purpose of the present study was to determine the effects of contaminated Baie des Anglais sediments on American plaice (Hippoglossoides platessoides) immune function. Three sites in Baie des Anglais were selected which vary in proximity to local industries and in their sediment contaminant load. Sites 1 and 2 (within the bay) are the closest to shore and most heavily contaminated while sediments at Site 3, which is outside the bay, are the least contaminated. In the first experiment, American plaice were placed in cages at each site for three weeks and immune function was assessed by measuring the phagocytic activity of pronephric macrophages. At the time of sampling, plaice displayed pronephros cell immune response disturbances indicating that Site 1 and 2 were most toxic and Site 3 the least toxic. The results obtained for phagocytosis revealed that contaminants present in the sediments are bioavailable to fish, which came in contact with them and significantly affected their immune system. In the second experiment, sediments from the most toxic site, Site 1, were collected for a laboratory controlled experiment in which plaice were exposed for up to 3 months to these contaminated marine sediments, while the control group was exposed to relatively uncontaminated beach sand. At the end of the exposure period, plaice were transferred from contaminated sediment to beach sand and sampled one month later in order to determine if immune function had returned to control levels. The total number of macrophages decreased following three months of exposure, while the active macrophages had already decreased after the first month of exposure. Following the rehabilitation period a significant trend toward normal response was noted. Sediments from Baie des Anglais contain primarily less highly chlorinated PCBs and lower concentrations of the intermediate and highly chlorinated PCBs. The total concentration of PCBs (sum of 20 congeners) in the contaminated sediments was 1500 ng/g while in the beach sand, the levels were 13.6 ng/g dry weight. Only the low chlorinated PCB congeners were efficiently transferred from the sediments to the plaice liver. Together, these results suggest that the effect of chemical exposure on the phagocytosis of plaice macrophages may be reversible if the fish are returned to a non-contaminated habitat.