Monitoring of Phenol in Wastewater Bioremediation by HPLC

Bioremediation emphasizes the detoxification and destruction of toxic substances by microorganisms. Wastewater obtained from an industrial concern was solvent extracted with methyl alcohol and dichloromethane and analysed by GC/MS. Besides phenol, a large variety of organic compounds were detected. Under controlled laboratory conditions, the wastewater was innoculated with a mixed culture of microorganisms specially selected for their abilities to degrade phenol. Samples were collected at regular intervals from the stirred tank bioreactor and analysed for phenol by reverse phase HPLC with a C18 column. Results shows that from an initial phenol concentration of 987 ppm, slightly more than 50% was destroyed within 163 hours. The dry weight of the microorganisms and the plate count (CFU/ml) shows a steady increase from 0.5238 gms to 0.5355 gms and from 1.1E+9 to 1.94E+13 respectively over the same period. This suggested that the phenol was consumed by the microorganisms as the sole carbon source.     

Biosolids application in the Chihuahuan desert: effects on runoff water quality

Surface-applied biosolids, the option most often used on range-lands, can increase the concentration of macronutrients and trace elements in the runoff water and can potentially produce eutrophication or contamination of surface waters. In this study, the effects of postapplication age of biosolids (18, 12, 6, and 0.5 mo) and rate of application (0, 7, 18, 34, and 90 Mg ha(-1)) on the quality of runoff water from shrubland and grassland soils were assessed. Between July and October 1996 simulated rainfall was applied to 0.50-m2 plots for 30 min at a rate of 160 mm h(-1). All of the runoff water was collected. The concentration of NH4+ -N, NO3- -N, PO4(3-)-P, total dissolved phosphorus (TDP), Cu, and Mn in the runoff water increased with rate of biosolids application and decreased with time of postapplication on the two soils. The highest PO4(3-)-P and NH4+ -N concentrations, 4.96 and 97 mg L(-1), respectively, were recorded in the grassland soil treated with 90 Mg ha(-1) of biosolids 0.5 mo postapplication. For the same soil, rate, and postapplication age of biosolids, Cu exceeded the upper limit (0.50 mg L(-1) in drinking water for livestock. Ammonium N and PO4(3-)-P should be the main compounds considered when surface-applying biosolids. Ammonium N at concentrations found in all biosolids-treated plots may affect the quality of livestock drinking water by causing taste and smell problems. Orthophosphate can contribute to eutrophication if the runoff from biosolids-treated areas enter surface waters.     

Leonardo da Vinci’s “Last Supper”: a case study to evaluate the influence of visitors on the Museum preservation systems

Environmental Science and Pollution Research - The most important parameter to obtain an appropriate preservation condition of museum environments concerns the indoor air quality. The exposure of...