The effect of titanium dioxide nanoparticles on neuroinflammation response in rat brain

Titanium dioxide nanoparticles (TiO₂ NPs) are widely used for their whiteness and opacity in several applications such as food colorants, drug additives, biomedical ceramic, and implanted biomaterials. Research on the neurobiological response to orally administered TiO₂ NPs is still limited. In our study, we investigate the effects of anatase TiO₂ NPs on the brain of Wistar rats after oral intake. After daily intragastric administration of anatase TiO₂ NPs (5–10 nm) at 0, 50, 100, and 200 mg/kg body weight (BW) for 60 days, the coefficient of the brain, acethylcholinesterase (AChE) activities, the level of interleukin 6 (IL-6), and the expression of glial fibrillary acidic protein (GFAP) were assessed to quantify the brain damage. The results showed that high-dose anatase TiO₂ NPs could induce a downregulated level of AChE activities and showed an increase in plasmatic IL-6 level as compared to the control group accompanied by a dose-dependent decrease inter-doses, associated to an increase in the cerebral IL-6 level as a response to a local inflammation in brain. Furthermore, we observed elevated levels of immunoreactivity to GFAP in rat cerebral cortex. We concluded that oral intake of anatase TiO₂ NPs can induce neuroinflammation and could be neurotoxic and hazardous to health.     

Long-term microphytoplankton variability patterns using multivariate analyses: ecological and management implications

Microphytoplankton data along the southern Tunisian coasts have been monitored weekly since 1995. This study used the data collected during the period 2000–2007 to determine the temporal variability patterns and to characterize the geographical structures of microphytoplankton populations. The methodological approach consisted in isolating the temporal variability common to all sampled sites and in analyzing the “residual” site-specific component. Multivariate ordination methods were used to determine the seasonal and interannual variability. The temporal pattern shared by all sites was highlighted and the seasonal cycle of the most frequent phytoplankton communities contrasted the diatoms winter-spring species to the dinoflagellates summer-fall species. Multitable comparison allowed the identification of three areas exhibiting similar phytoplankton population variability. The results, despite the coastal location of sampling sites, indicated that hydrodynamic and geomorphologic properties of the different areas as well as anthropogenic activities play a key role in the structure of the phytoplankton communities.     

Genotoxicity associated with oxidative damage in the liver and kidney of mice exposed to dimethoate subchronic intoxication

Background and aims  

Because of the widespread use of pesticides for domestic and industrial applications, the evaluation of their toxic effects is of major concern to public health. The aim of the present study was to investigate the propensity of dimethoate (DM), an organophosphorus pesticide, to cause oxidative damage in the liver and kidney of mice and its associated genotoxic effect.     

Evaluating the phytoremediation potential of Phragmites australis grown in pentachlorophenol and cadmium co-contaminated soils

Pot-culture experiments were conducted to evaluate the phytoremediation potential of a wetland plant species, Phragmites australis in cadmium (Cd) and pentachlorophenol (PCP) co-contaminated soil under glasshouse conditions for 70 days. The treatments included Cd (0, 5 and 50 mg kg^?1) without or with PCP (50 and 250 mg kg^?1). The results showed that growth of P. australis was significantly influenced by interaction of Cd and PCP, decreasing with either Cd or PCP additions. Plant biomass was inhibited and reduced by the rate of 89 and 92 % in the low and high Cd treatments and by 20 and 40 % in the low and high PCP treatments compared to the control. The mixture of low Cd and low PCP lessened Cd toxicity to plants, resulting in improved plant growth (by 144 %). Under the joint stress of the two contaminants, the ability of Cd uptake and translocation by P. australis was weak, and the BF and TF values were inferior to 1.0. A low proportion of the metal is found aboveground in comparison to roots, indicating a restriction on transport upwards and an excluding effect on Cd uptake. Thus, P. australis cannot be useful for phytoextraction. The removal rate of PCP increased significantly (70 %) in planted soil. Significant positive correlations were found between the DHA and the removal of PCP in planted soils which implied that plant root exudates promote the rhizosphere microorganisms and enzyme activity, thereby improving biodegradation of PCP. Based on results, P. australis cannot be effective for phytoremediation of soil co-contaminated with Cd and PCP. Further, high levels of pollutant hamper and eventually inhibit plant growth. Therefore, developing supplementary methods (e.g. exploring the partnership of plant–microbe) for either enhancing (phytoextraction) or reducing the bioavailability of contaminants in the rhizosphere (phytostabilization) as well as plant growth promoting could significantly improve the process of phytoremediation in co-contaminated soil.     

Zearalenone metabolism and excretion in the rat: effect of different doses

Young female rats were orally dosed with either 1 or 100 mg zearalenone kg-1 body weight; zearalenone and metabolites were measured in a 96-h collection of urine and feces by HPLC analysis. Dose had little effect on metabolites formed, or excretion route. In both treatment groups, about 55% of the oral dose was excreted in the feces, while the urine was also a major route of excretion accounting for 15-20% of the administered dose. Zearalenone and metabolites were excreted mainly in the free form, with the production of alpha-zearalenol, the most potent estrogenic metabolite, being greater than 10% of the zearalenone dose.     

Photocatalytic and biodegradation treatments of paracetamol: investigation of the in vivo toxicity

Environmental Science and Pollution Research - Medicines and drugs consumption by all populations of the world can be expected to result in the contamination of the environment since 30–90%...     

Microbiological parameters and maturity degree during composting of Posidonia oceanica residues mixed with vegetable wastes in semi-arid pedo-climatic condition

The aim of this study was to characterize the biological stability and maturity degree of compost during a controlled pile-composting trial of mixed vegetable residues (VR) collected from markets of Tunis City with residues of Posidonia oceanica (PoR), collected from Tunis beaches. The accumulation in beaches (as well as their removal) constitutes a serious environmental problem in all Mediterranean countries particularly in Tunisia. Aerobic-thermophilic composting is the most reasonable way to profit highly-valuable content of organic matter in these wastes for agricultural purposes. The physical, chemical, and biological parameters were monitored during composting over 150 d. The most appropriate parameters were selected to establish the maturity degree. The main result of this research was the deduction of the following maturity criterion: (a) C/N ratio < 15; (b) NH₄⁺-N < 400 mg/kg; (c) CO₂-C < 2000 mg CO₂-C/kg; (d) dehydrogenase activity < 1 mg TPF/g dry matter; (e) germination index (GI) > 80%. These five parameters, considered jointly are indicative of a high maturity degree and thus of a high-quality organic amendment which employed in a rational way, may improve soil fertility and soil quality. The mature compost was relatively rich in N (13.0 g/kg), P (4.74 g/kg) and MgO (15.80 g/kg). Thus composting definitively constitutes the most optimal option to exploit these wastes.     

Microbial biomass in a soil amended with different types of organic wastes.

Application of different types of organic wastes may have a marked effect on soil microbial biomass and its activity. The objective of this study was to quantify the amount of microbial biomass in a loamy-clayey soil, amended with different types of organic waste residues (composts of municipal solid waste of different ages, sewage sludge and farmyard manure) and incubated for 8 weeks at 25 degrees C and two-thirds of field capacity, using the fumigation-extraction method. Both microbial biomass-C and -N (BC and BN, respectively) appeared to be dependent on the type of organic waste residues, on their degree of stability, and on their chemical characteristics. In general, organic wastes increased the microbial biomass-C content in the soil and the microbial BC was positively correlated with the organic C content, the C/N, neutral detergent fibre/N (NDF/N) and acid detergent fibre/N (ADF/ N) ratios. The microbial biomass content decreased according to the period of incubation, especially when the compost used was immature. The microbial biomass-N was positively correlated with the total N and percentage of hemicellulose. The microbial biomass-C was linearly related with the microbial biomass-N and the ratio BC/BN was exponentially related with the BC.