Growth, yield and elements content of wheat (Triticum aestivum) grown in composted municipal solid wastes amended soil

A commercial formulation of composted municipal solid wastes (MSW) was used for amending soil at 0, 50, 100, 150, 200 and 250 kg ha⁻¹ in which wheat had been grown (field experiments) and element residues of amended soil and plant parts were enumerated. MSW amendment caused a significant improvement in soil quality. Growth (shoot length, leaf number, leaf area, tiller number, plant dry weight and chlorophyll contents of leaves) and yield (length of panicle, number of panicles per plant and grain yield per plant) of wheat increased gradually up to the MSW-amendment level of 200 kg ha⁻¹. Elements, Ni, Zn, Cu, Cd, Cr, and Pb accumulated in plants from MSW amended soil, but the degree of metal accumulation was the least in seeds in comparison to other plant parts (root, stem and leaf). Moreover, Ni, Zn, Cd and Pb, were in high concentration in all plant parts. It is recorded that the level of 200 kg ha⁻¹ MSW amendment caused better growth and yield of wheat, but progressive levels of metal accumulation in plant parts were recorded due to increase in amendment levels. Readers should send their comments on this paper to: BhaskarNath@aol.com within 3 months of publication of this issue.     

Carbon sequestration in reclaimed manganese mine land at Gumgaon, India

Carbon emission is supposed to be the strongest factor for global warming. Removing atmospheric carbon and storing it in the terrestrial biosphere is one of the cost-effective options, to compensate greenhouse gas emission. Millions of acres of abandoned mine land throughout the world, if restored and converted into vegetative land, would solve two major problems of global warming and generation of degraded wasteland. In this study, a manganese spoil dump at Gumgaon, Nagpur in India was reclaimed, using an integrated biotechnological approach (IBA). The physicochemical and microbiological status of the mine land improved after reclamation. Soil organic carbon (SOC) pool increased from 0.104% to 0.69% after 20 years of reclamation in 0–15 cm spoil depth. Soil organic carbon level of reclaimed site was also compared with a native forestland and agricultural land. Forest soil showed highest SOC level of 1.11% followed by reclaimed land and agriculture land of 0.70% and 0.40%, respectively. Soil profile studies of all three sites showed that SOC pool decreased from 0–15, 15–30, and 30–45 cm depths. Although reclaimed land showed less carbon than forestland, it showed better SOC accumulation rate. Reclamation of mine lands by using IBA is an effective method for mitigating CO₂ emissions.     

Smokeless tobacco extract impairs iron homeostasis in rats and human hepatoma,HepG2 cells

Tobacco exposure may alter homeostasis of iron (Fe), one of the most abundant and essential transition metals in the body. In this study, the effect of aqueous extract of smokeless tobacco was evaluated on Fe homeostasis in rats and human hepatoma, HepG2 cells. Our findings suggested that tobacco consumption even at low doses impairs Fe homeostasis leading to Fe deficiency anemia. Significant alterations were noted with respect to hematological parameters and expression patterns of selected intestinal Fe-transporters, Fe-binding proteins, and Fe-regulatory hormone, hepcidin. Impairment in the hepatic and renal antioxidant defense system was also observed in the treated rats. Histopathological studies revealed cirrhosis of liver and goblet cell hyperplasia of small intestine. Further, analysis of hepcidin promoter and its expression along with ferritin (expression and ELISA) in HepG2 cells demonstrated an enhanced expression of both the genes resulting in sequestration of Fe in treated cells, thus indicating systemic Fe deficiency.     

Performance Evaluation of Human-Specific Viral Markers and Application of Pepper Mild Mottle Virus and CrAssphage to Environmental Water Samples as Fecal Pollution Markers in the Kathmandu Valley,Nepal

Monitoring of environmental water is crucial to protecting humans and animals from possible health risks. Although numerous human-specific viral markers have been designed to track the presence of human fecal contamination in water, they lack adequate sensitivity and specificity in different geographical regions. We evaluated the performances of six human-specific viral markers [Aichi virus 1 (AiV-1), human adenoviruses (HAdVs), BK and JC polyomaviruses (BKPyVs and JCPyVs), pepper mild mottle virus (PMMoV), and crAssphage] using 122 fecal-source samples collected from humans and five animal hosts in the Kathmandu Valley, Nepal. PMMoV and crAssphage showed high sensitivity (90–100%) with concentrations of 4.5–9.1 and 6.2–7.0 log₁₀ copies/g wet feces (n = 10), respectively, whereas BKPyVs, JCPyVs, HAdVs, and AiV-1 showed poor performances with sensitivities of 30–40%. PMMoV and crAssphage were detected in 40–100% and 8–90%, respectively, of all types of animal fecal sources and showed no significantly different concentrations among most of the fecal sources (Kruskal–Wallis test, P > 0.05), suggesting their applicability as general fecal pollution markers. Furthermore, a total of 115 environmental water samples were tested for PMMoV and crAssphage to identify fecal pollution. PMMoV and crAssphage were successfully detected in 62% (71/115) and 73% (84/115) of water samples, respectively. The greater abundance and higher mean concentration of crAssphage (4.1 ± 0.9 log₁₀ copies/L) compared with PMMoV (3.3 ± 1.4 log₁₀ copies/L) indicated greater chance of detection of crAssphage in water samples, suggesting that crAssphage could be preferred to PMMoV as a marker of fecal pollution.

     

Selenium speciation in wheat grain varies in the presence of nitrogen and sulphur fertilisers

This study investigated whether selenium species in wheat grains could be altered by exposure to different combinations of nitrogen (N) and sulphur (S) fertilisers in an agronomic biofortification experiment. Four Australian wheat cultivars (Mace, Janz, Emu Rock and Magenta) were grown in a glasshouse experiment and exposed to 3 mg Se kg^?1 soil as selenate (Se^VI). Plants were also exposed to 60 mg N kg^?1 soil as urea and 20 mg S kg^?1 soil as gypsum in a factorial design (N + S + Se; N + Se; S + Se; Se only). Plants were grown to maturity with grain analysed for total Se concentrations via ICP-MS and Se species determined via HPLC-ICP-MS. Grain Se concentrations ranged from 22 to 70 µg Se g^?1 grain (dry mass). Selenomethionine (SeMet), Se-methylselenocystine (MeSeCys), selenohomolanthionine (SeHLan), plus a large concentration of uncharacterised Se species were found in the extracts from grains. SeMet was the major Se species identified accounting for between 9 and 24 µg Se g^?1 grain. Exposure to different N and S fertiliser combinations altered the SeMet content of Mace, Janz and Emu Rock grain, but not that of Magenta. MeSeCys and SeHLan were found in far lower concentrations (<4 µg Se g^?1 grain). A large component of the total grain Se was uncharacterisable (>30 % of total grain Se) in all samples. When N fertiliser was applied (with or without S), the proportion of uncharacterisable Se increased between 60 and 70 % of the total grain Se. The data presented here indicate that it is possible to alter the content of individual Se species in wheat grains via biofortification combined with manipulation of N and S fertiliser regimes. This has potential significance in alleviating or combating both Se deficiency and Se toxicity effects in humans.     

Disruption of zebrafish (Danio rerio) reproduction upon chronic exposure to TiO₂ nanoparticles

As common engineered nanomaterials, TiO(2) nanoparticles (nTiO(2)) are usually perceived as non-toxic, and have already been widely used in many products and applications. Such a perception might have been shaped by some short-term studies that revealed no/low toxicity of nTiO(2) to cells and eco-relevant organisms. However, given the ultimate release of nTiO(2) into the aquatic environment, which can act as a sink for engineered nanoparticles, their long-term impact on the environment and human health is still a concern and deserves more research efforts. Here, for the first time, we demonstrate that chronic exposure of zebrafish to 0.1 mg L(-1) nTiO(2), can significantly impair zebrafish reproduction. For instance, there was a 29.5% reduction in the cumulative number of zebrafish eggs after 13 weeks of nTiO(2) exposure. Thus, we provided timely information on indicating a serious risk of reproductive impairment of environments contaminated with low levels of nTiO(2) on aquatic organisms, leading to alterations in population dynamics and aquatic ecosystem balance, and thus warrants a careful scrutiny on toxicity assessment of nTiO(2), especially their long-term impact.