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Kumar S Dagar SS Mohanty AK Sirohi SK Puniya M Kuhad RC Sangu KP Griffith GW Puniya AK 《Die Naturwissenschaften》2011,98(6):457-472
Methanogens, the members of domain Archaea are potent contributors in global warming. Being confined to the strict anaerobic environment, their direct cultivation as
pure culture is quite difficult. Therefore, a range of culture-independent methods have been developed to investigate their
numbers, substrate uptake patterns, and identification in complex microbial communities. Unlike other approaches, fluorescence
in situ hybridization (FISH) is not only used for faster quantification and accurate identification but also to reveal the
physiological properties and spatiotemporal dynamics of methanogens in their natural environment. Aside from the methodological
aspects and application of FISH, this review also focuses on culture-dependent and -independent techniques employed in enumerating
methanogens along with associated problems. In addition, the combination of FISH with micro-autoradiography that could also
be an important tool in investigating the activities of methanogens is also discussed. 相似文献
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