Spectrophotometric study of DNA interactions with ftorafur and its elementoorganic derivatives

Ftorafur is an antimetabolite antitumour drug successfully used for treatment of various tumours. It is generally accepted that ftorafur is converted to 5-fluoruracil. However, some data indicate direct interactions of the compound with DNA. To test this hypothesis we have performed spectrophotometric study of DNA interactions of ftorafur and some of its elementoorganic derivatives with DNA. UV-VIS spectra of the tested compounds were recorded in absence and presence of increasing amounts of DNA. DNA caused a hypochromic effect in spectra of ftorafur, similar, but weaker effect was observed in 5-fluoruracil spectra. Trimethylgermyl derivative of ftorafur manifested a higher DNA-binding capacity compared to ftorafur. To reveal possible mechanism of interaction between the tested nucleosides and DNA ethidium bromide extrusion experiments were performed. It was shown that the compounds did not compete with EBr for intercalation. Fourier transformation infrared spectroscopy analyses revealed decrease in intensity of several bands in spectra of ftorafur, its trimethylgermyl derivative and fluorouracil in presence of DNA indicating again interactions between the compounds and DNA. Docking experiments reveal interactions of the tested nucleosides with the DNA minor groove. Thus ftorafur is capable to interact directly with DNA; further modifications of the molecule enhance this capacity.     

Waste paper and clinoptilolite as a bulking material with dewatered anaerobically stabilized primary sewage sludge (DASPSS) for compost production

Environmental problems associated with sewage sludge disposal have prompted strict legislative actions over the past few years. At the same time, the upgrading and expansion of wastewater treatment plants have greatly increased the volume of sludge generated. The major limitation of land application of sewage sludge compost is the potential for high heavy metal content in relation to the metal content of the original sludge. Composting of sewage sludge with natural zeolite (clinoptilolite) can enhance its quality and suitability for agricultural use. However, the dewatered anaerobically stabilized primary sewage sludge (DASPSS) contained a low concentration of humic substances (almost 2%), and the addition of the waste paper was necessary in order to produce a good soil conditioner with high concentrations of humics. The final results showed that the compost produced from DASPSS and 40-50% w/w of waste paper was a good soil fertilizer. Finally, in order to estimate the metal leachability of the final compost product, the generalized acid neutralization Capacity (GANC) procedure was used, and it was found that by increasing the leachate pH, the heavy metal concentration decreased. The application of the sequential chemical extraction indicated that metals were bound to the residual fraction characterized as a stabilize fractions.     

Modeling the mobility of glyphosate from two contrasting agricultural soils in laboratory column experiments

The method of single-strand conformational polymorphism (SSCP) was modified in our laboratories for the characterization of baculoviruses, insect viruses with great potential for use as bioinsecticides in biological protection programs. A series of primers were synthesized after the comparison of the polyhedrin gene sequences of over 20 baculoviruses. Polyhedrin is a highly conserved protein which is responsible for the persistence of the virus in the environment. Universal primers were designed which could be used in polymerase chain reactions (PCR) containing genomic DNA from an array of nucleopolyhedrosis viruses (NPVs) including these which are used as biopesticides against important pests of forests and crops, such as Anticarsia gemmatalis, Spodoptera frugiperda, Lymantria dispar, Lymantria monacha and many others. PCR products were denatured and subjected to single-strand DNA electrophoresis at variable temperatures (MSSCP) where, after silver staining, they gave ssDNA band patterns characteristic for each baculovirus species. This technique can be potentially applied to detect baculoviruses in insects collected in the field, as well as to plant tissues and the excrements or bodies of predators without need for sequencing the PCR products. Sometimes MSSCP can be used not only for species determination but also as an indication of genomic variability which can be related to infectivity.     

Heteroscedastic semiparametric models for domestic water consumption aggregated data

Heteroscedastic additive and multiplicative models are proposed to disaggregate household data on water consumption from Athens and provide individual consumption estimates. The models adjust for heteroscedasticity assuming that variances relate to covariates. Household characteristics that can influence consumption are also included into models in order to allow for a clearer measurement of individual characteristics effects. Estimation is accomplished through a penalized least squares approach. The method is applied to a sample of real data related to domestic water consumption in Athens. The results show a greater consumption of water for males while the single-female households are these that use the lowest quantities of water. The consumption curves by age and gender are constructed presenting differences between the two sexes.

Ranking and selecting dangerous crash locations: correcting for the number of passengers and Bayesian ranking plots

INTRODUCTION: In this paper a sensitivity analysis is performed to investigate how big the impact would be on the current ranking of crash locations in Flanders (Belgium) when only taking into account the most serious injury per crash instead of all the injured occupants. RESULTS: Results show that this would lead to a different selection of 23.8% of the 800 sites that are currently considered as dangerous. CONCLUSIONS: Considering this impact quantity, the researchers want to sensitize government that giving weight to the severity of the crash can correct for the bias that occurs when the number of occupants of the vehicles are subject to coincidence. Additionally, probability plots are generated to provide policy makers with a scientific instrument with intuitive appeal to select dangerous road locations on a statistically sound basis. Impact on industry Considering the impact quantity of giving weight to the severity of the crash instead of to all the injured occupants of the vehicle on the ranking of crash sites, the authors want to sensitize government to carefully choose the criteria for ranking and selecting crash locations in order to achieve an enduring and successful traffic safety policy. Indeed, giving weight to the severity of the crash can correct for the bias that occurs when the number of occupants of the vehicles are subject to coincidence. However, it is up to the government to decide which priorities should be stressed in the traffic safety policy. Then, the appropriate weighting value combination can be chosen to rank and select the most dangerous crash locations. Additionally, the probability plots proposed in this paper can provide policy makers with a scientific instrument with intuitive appeal to select dangerous road locations on a statistically sound basis. Note that, in practice, one should not only rank the crash locations based on the benefits that can be achieved from tackling these locations. Future research is also needed to incorporate the costs of infrastructure measures and other actions that these crash sites require in order to enhance the safety on these locations. By balancing these costs and benefits against each other, the crash locations can then be ranked according to the order in which they should be prioritized.