Monitoring of Phenol in Wastewater Bioremediation by HPLC

Bioremediation emphasizes the detoxification and destruction of toxic substances by microorganisms. Wastewater obtained from an industrial concern was solvent extracted with methyl alcohol and dichloromethane and analysed by GC/MS. Besides phenol, a large variety of organic compounds were detected. Under controlled laboratory conditions, the wastewater was innoculated with a mixed culture of microorganisms specially selected for their abilities to degrade phenol. Samples were collected at regular intervals from the stirred tank bioreactor and analysed for phenol by reverse phase HPLC with a C18 column. Results shows that from an initial phenol concentration of 987 ppm, slightly more than 50% was destroyed within 163 hours. The dry weight of the microorganisms and the plate count (CFU/ml) shows a steady increase from 0.5238 gms to 0.5355 gms and from 1.1E+9 to 1.94E+13 respectively over the same period. This suggested that the phenol was consumed by the microorganisms as the sole carbon source.     

Estimating Forest Ecosystem Evapotranspiration at Multiple Temporal Scales With a Dimension Analysis Approach1

Abstract:  It is critical that evapotranspiration (ET) be quantified accurately so that scientists can evaluate the effects of land management and global change on water availability, streamflow, nutrient and sediment loading, and ecosystem productivity in watersheds. The objective of this study was to derive a new semi‐empirical ET modeled using a dimension analysis method that could be used to estimate forest ET effectively at multiple temporal scales. The model developed describes ET as a function of water availability for evaporation and transpiration, potential ET demand, air humidity, and land surface characteristics. The model was tested with long‐term hydrometeorological data from five research sites with distinct forest hydrology in the United States and China. Averaged simulation error for daily ET was within 0.5 mm/day. The annual ET at each of the five study sites were within 7% of measured values. Results suggest that the model can accurately capture the temporal dynamics of ET in forest ecosystems at daily, monthly, and annual scales. The model is climate‐driven and is sensitive to topography and vegetation characteristics and thus has potential to be used to examine the compounding hydrologic responses to land cover and climate changes at multiple temporal scales.     

Interaction Effects of Metals and Salinity on Biodegradation of a Complex Hydrocarbon Waste

Abstract

The presence of high levels of salts because of produced brine water disposal at flare pits and the presence of metals at sufficient concentrations to impact microbial activity are of concern to bioremediation of flare pit waste in the upstream oil and gas industry. Two slurry-phase biotreatment experiments based on three-level factorial statistical experimental design were conducted with a flare pit waste. The experiments separately studied the primary effect of cadmium [Cd(II)] and interaction effect between Cd(II) and salinity and the primary effect of zinc [Zn(II)] and interactioneffect between Zn(II) and salinity on hydrocarbon biodegradation. The results showed 42–52.5% hydrocarbon removal in slurries spiked with Cd and 47–62.5% in the slurries spiked with Zn. The analysis of variance showed that the primary effects of Cd and Cd–salinity interaction were statistically significant on hydrocarbon degradation. The primary effects of Zn and the Zn-salinity interaction were statistically insignificant, whereas the quadratic effect of Zn was highly significant on hydrocarbon degradation. The study on effects of metallic chloro-complexes showed that the total aqueous concentration of Cd or Zn does not give a reliable indication of overall toxicity to the microbial activity in the presence of high salinity levels.     

Interaction effects of metals and salinity on biodegradation of a complex hydrocarbon waste

The presence of high levels of salts because of produced brine water disposal at flare pits and the presence of metals at sufficient concentrations to impact microbial activity are of concern to bioremediation of flare pit waste in the upstream oil and gas industry. Two slurry-phase biotreatment experiments based on three-level factorial statistical experimental design were conducted with a flare pit waste. The experiments separately studied the primary effect of cadmium [Cd(II)] and interaction effect between Cd(II) and salinity and the primary effect of zinc [Zn(II)] and interaction effect between Zn(II) and salinity on hydrocarbon biodegradation. The results showed 42-52.5% hydrocarbon removal in slurries spiked with Cd and 47-62.5% in the slurries spiked with Zn. The analysis of variance showed that the primary effects of Cd and Cd-salinity interaction were statistically significant on hydrocarbon degradation. The primary effects of Zn and the Zn-salinity interaction were statistically insignificant, whereas the quadratic effect of Zn was highly significant on hydrocarbon degradation. The study on effects of metallic chloro-complexes showed that the total aqueous concentration of Cd or Zn does not give a reliable indication of overall toxicity to the microbial activity in the presence of high salinity levels.     

Bioaccumulation and depuration of chromium in the selected organs and whole body tissues of freshwater fish Cirrhinus mrigala individually and in binary solutions with nickel

Contamination of aquatic ecosystems with heavy metals has been receiving increased worldwide attention due to their harmful e ects on human health and other organisms in the environment. Most of the studies dealing with toxic e ects of metals deal with single metal species, while the aquatic organisms are typically exposed to mixtures of metals. Hence, in order to provide data supporting the usefulness of freshwater fish as indicators of heavy metal pollution, it has been proposed in the present study to investigate the bioaccumulation and depuration of chromium in the selected organs of freshwater fingerlings Cirrhinus mrigala, individually and in binary solutions with nickel. The results show that the kidney is a target organ for chromium accumulation, which implies that it is also the “critical” organ for toxic symptoms. The results further show that accumulation of nickel in all the tissues of C. mrigala is higher than that of chromium. In addition, the metal accumulations of the binary mixtures of chromium and nickel are substantially higher than those of the individual metals, indicating synergistic interactions between the two metals. Theoretically the simplest explanation for an additive joint action of toxicants in a mixture is that they act in a qualitatively similar way. The observed data suggest that C. mrigala could be suitable monitoring organisms to study the bioavailability of water-bound metals in freshwater habitats.     

Characterization of Storm Flow Dynamics of Headwater Streams in the South Carolina Lower Coastal Plain1

Epps, Thomas H., Daniel R. Hitchcock, Anand D. Jayakaran, Drake R. Loflin, Thomas M. Williams, and Devendra M. Amatya, 2012. Characterization of Storm Flow Dynamics of Headwater Streams in the South Carolina Lower Coastal Plain. Journal of the American Water Resources Association (JAWRA) 1‐14. DOI: 10.1111/jawr.12000 Abstract: Hydrologic monitoring was conducted in two first‐order lower coastal plain watersheds in South Carolina, United States, a region with increasing growth and land use change. Storm events over a three‐year period were analyzed for direct runoff coefficients (ROC) and the total storm response (TSR) as percent rainfall. ROC calculations utilized an empirical hydrograph separation method that partitioned total streamflow into sustained base flow and direct runoff components. ROC ratios ranged from 0 to 0.32 on the Upper Debidue Creek (UDC) watershed and 0 to 0.57 on Watershed 80 (WS80); TSR results ranged from 0 to 0.93 at UDC and 0.01 to 0.74 at WS80. Variability in event runoff generation was attributed to seasonal trends in water table elevation fluctuation as regulated by evapotranspiration. Groundwater elevation breakpoints for each watershed were identified based on antecedent water table elevation, streamflow, ROCs, and TSRs. These thresholds represent the groundwater elevation above which event runoff generation increased sharply in response to rainfall. For effective coastal land use decision making, baseline watershed hydrology must be understood to serve as a benchmark for management goals, based on both seasonal and event‐based surface and groundwater interactions.     

Curve Number Derivation for Watersheds Draining Two Headwater Streams in Lower Coastal Plain South Carolina,USA

The objective of this study was to assess curve number (CN) values derived for two forested headwater catchments in the Lower Coastal Plain (LCP) of South Carolina using a three‐year period of storm event rainfall and runoff data in comparison with results obtained from CN method calculations. Derived CNs from rainfall/runoff pairs ranged from 46 to 90 for the Upper Debidue Creek (UDC) watershed and from 42 to 89 for the Watershed 80 (WS80). However, runoff generation from storm events was strongly related to water table elevation, where seasonally variable evapotranspirative wet and dry moisture conditions persist. Seasonal water table fluctuation is independent of, but can be compounded by, wet conditions that occur as a result of prior storm events, further complicating flow prediction. Runoff predictions for LCP first‐order watersheds do not compare closely to measured flow under the average moisture condition normally associated with the CN method. In this study, however, results show improvement in flow predictions using CNs adjusted for antecedent runoff conditions and based on water table position. These results indicate that adaptations of CN model parameters are required for reliable flow predictions for these LCP catchments with shallow water tables. Low gradient topography and shallow water table characteristics of LCP watersheds allow for unique hydrologic conditions that must be assessed and managed differently than higher gradient watersheds.     

Bioaccumulation of lead and the influence of chelating agents in Catla catla fingerlings

Lead is a widespread element and one of the persistent and cumulative pollutants of the environment. The present study deals with the bioaccumulation of lead and the influence of chelating agents, meso 2,3-dimercaptosuccinic acid (DMSA), D-Penicillamine and CaNa₂EDTA in reducing the concentration of lead on the selected organs of Catla catla fingerlings for both acute and chronic exposures by using ICP-AES. It is inferred from the present findings that there was a correlation between environmental conditions and the heavy metal contents of the fish. The highest concentration of lead is found in kidney tissues and the lowest in muscle tissues. The accumulation pattern of lead in the selected organs of Catla catla is: kidney > liver > gill > brain > muscle. Also, it has been found that the treatment of chelating agents, DMSA, D-Penicillamine and CaNa₂EDTA reduces the concentration of lead significantly for both acute and chronic exposures. The results also show that DMSA is the most effective chelator of lead in reducing the body burden of C. catla fingerlings. The observed data further indicate that C. catla could be suitable for monitoring organisms to study the bioavailability of water-bound metals in freshwater habitats.     

EFFECTS OF CONTROLLED DRAINAGE ON STORM EVENT HYDROLOGY IN A LOBLOLLY PINE PLANTATION1

ABSTRACT: A paired watershed approach was utilized to study the effects of three water management regimes on storm event hydrology in three experimental watersheds in a drained loblolly pine (Pinus taeda L.) plantation in eastern North Carolina. The regimes were: (1) conventional drainage, (2) controlled drainage (CD) to reduce outflows during spring fish recruitment, and (3) controlled drainage to reduce outflows and conserve water during the growing season. Data from two pit‐treatment years and three years of CD treatment with raised weirs at the watershed outlet are presented. CD treatment resulted in rises in water table elevations during the summer. But the rises were small and short‐lived due to increased evapotranspiration (ET) rates as compared to the spring treatment with lower ET demands. CD treatment had no effect on water tables deeper than 1.3 m. CD treatments, however, significantly (α= 0.05) reduced the stoning outflows for all events, and peak outflow rates for most of the events depending upon the outlet weir level. In some events, flows did not occur at all in watersheds with CD. When event outflows occurred, duration of the event was sharply reduced because of reduced effective ditch depth. Water table depth at the start of an event influenced the effect of CD treatment on storm event hydrology.     

Hydrology and Water Budget for a Forested Atlantic Coastal Plain Watershed,South Carolina1

Abstract: Increases in timber demand and urban development in the Atlantic Coastal Plain over the past decade have motivated studies on the hydrology, water quality, and sustainable management of coastal plain watersheds. However, studies on baseline water budgets are limited for the low‐lying, forested watersheds of the Atlantic Coastal Plain. The purpose of this study was to document the hydrology and a method to quantify the water budget of a first‐order forested watershed, WS80, located within the USDA Forest Service Santee Experimental Forest northeast of Charleston, South Carolina. Annual Rainfall for the 2003 and 2004 periods were 1,671 mm (300 mm above normal) and 962 mm (over 400 mm below normal), respectively. Runoff coefficients (outflow as a fraction of total rainfall) for the 2003 and 2004 periods were 0.47 and 0.08, respectively, indicating a wide variability of outflows as affected by antecedent conditions. A spreadsheet‐based Thornthwaite monthly water balance model was tested on WS80 using three different potential evapotranspiration estimators [Hamon, Thornthwaite, and Penman‐Monteith (P‐M)]. The Hamon and P‐M‐based methods performed reasonably well with average absolute monthly deviations of 12.6 and 13.9 mm, respectively, between the measured and predicted outflows. Estimated closure errors were all within 9% for the 2003, 2004, and seasonal water budgets. These results may have implications on forest management practices and provide necessary baseline or reference information for Atlantic Coastal Plain watersheds.