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Vah Mélanie Jarno Armelle Le Bot Sophie Ferret Yann Marin François 《Environmental Fluid Mechanics》2020,20(4):1031-1052
Environmental Fluid Mechanics - Most studies on sediment transport and bedforms migration consider unlimited sediment supply conditions. However, areas where the sediment supply is limited are... 相似文献
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Joke Muys Yves Jacquemyn Bettina Blaumeiser Laura Bourlard Nathalie Brison Saskia Bulk Patrizia Chiarappa Anne De Leener Marjan De Rademaeker Julie Désir Anne Destrée Koenraad Devriendt Annelies Dheedene Armelle Duquenne Annelies Fieuw Erik Fransen Jean-Stéphane Gatot Mauricette Jamar Sandra Janssens Jorien Kerstjens Kathelijn Keymolen Damien Lederer Björn Menten Bruno Pichon Sonia Rombout Yves Sznajer Ann Van Den Bogaert Kris Van Den Bogaert Joris Vermeesch Katrien Janssens 《黑龙江环境通报》2020,40(10):1272-1283
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Epidemiological and experimental studies have underlined that exposure to particulate matter (PM) leads mainly to airway inflammation, but the roles of particle size and chemical composition associated to such adverse health outcomes need to be better investigated. This study was performed to validate novel strategies of particle sampling, recovery and cell exposure in order to evaluate the pro-inflammatory potential of fine and ultrafine particles from a fractionated aerosol. Samplings of Paris background aerosols using 13-stage low pressure impactors (0.03-10 microm) gave bimodal mass distributions with an accumulation mode centered on a median diameter of 0.42 microm and a coarse one on 3.25 microm. PM 1 accounted for 70% and PM 0.1 for 12% of PM 10. The latter mainly comprised carbon-chained aggregates. The development of an efficient and reproducible method to recover fine (PM 1-0.1) and ultrafine (PM 0.1-0.03) particulate matter has permitted experimental comparison of the impact of such particles on human bronchial epithelial cells (HBECs). In this study we have compared the relative effects of fine and ultrafine particles at non-cytotoxic concentrations over 24h on the production of the pro-inflammatory cytokine GM-CSF by HBECs. Combining two cell exposure strategies to the size-fraction particles according to either their proportion (isovolume exposure) or their quantity in the aerosol (isomass exposure), we showed that both ultrafine and fine particles induced a concentration-dependent GM-CSF release by HBECs which is significant from 1 microg cm(-2). In conclusion, short duration samplings using 13-stage impactors enable to obtain size-resolved PM in sufficient quantities to carry out toxicological investigations. These findings are promising in view to conduct a more intensive study joining chemical and toxicological assays. 相似文献
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Armelle Braud Karine Jézéquel Emilie Vieille Adrien Tritter Thierry Lebeau 《Water, Air, & Soil Pollution: Focus》2006,6(3-4):261-279
Partly because of the low bioavailability of metals, the soil cleaning-up using phytoremediation is usually time-consuming.
In order to enhance the amount of metals at the plant's disposal, the soil bioaugmentation coupled together with phytoextraction
is an emerging technology. In this preliminary work, two agricultural soils which mainly differed in their Cr, Hg and Pb contents
(LC, low-contaminated soil; HC, high-contaminated soil) were bioaugmented in laboratory conditions by either bacterial (Bacillus subtilis, Pseudomonas aeruginosa, Pseudomonas fluorescens or Ralstonia metallidurans) or fungal inocula (Aspergillus niger or Penicillium simplicissimum) and incubated during three weeks. The LC soil pots bioaugmented with A. niger and P. aeruginosa contained higher concentrations of Cr (0.08 and 0.25 mg.kg−1 dw soil) and Pb (0.25 and 0.3 mg.kg−1 dw soil) in the exchangeable fraction F1 (extraction with MgCl2) by comparison with the non-bioaugmented soil where neither Cr nor Pb was detected. Conversely, immobilization of Cr and
Pb in the soil were observed with the other microorganisms. The soil bioaugmentation not only modified the metal speciation
for the most easily extractable fractions but also modified the distribution of metals in the other fractions, to a lesser
extent nevertheless. The difference in microbial concentrations between the bioaugmented or not HC soils reached up to 1.8
log units. Thus the microorganisms that we chose for the soil bioaugmentation were competitive towards the indigenous microflora.
The PCA analysis showed close positive relationships between the microorganisms which potentially produced siderophores in
the soil and the amount of Cr and Pb in the fraction F1. 相似文献
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Dalel Daâssi Lassaad Belbahri Armelle Vallat Steve Woodward Moncef Nasri Tahar Mechichi 《Environmental science and pollution research international》2014,21(3):1746-1758
The search for novel microorganisms able to degrade olive mill wastewaters (OMW) and withstand the toxic effects of the initially high phenolic concentrations is of great scientific and industrial interest. In this work, the possibility of reducing the phenolic content of OMW using new isolates of fungal strains (Coriolopsis gallica, Bjerkandera adusta, Trametes versicolor, Trichoderma citrinoviride, Phanerochaete chrysosporium, Gloeophyllum trabeum, Trametes trogii, and Fusarium solani) was investigated. In vitro, all fungal isolates tested caused an outstanding decolorization of OMW. However, C. gallica gave the highest decolorization and dephenolization rates at 30 % v/v OMW dilution in water. Fungal growth in OMW medium was affected by several parameters including phenolic compound concentration, nitrogen source, and inoculum size. The optimal OMW medium for the removal of phenolics and color was with the OMW concentration (in percent)/[(NH4)2SO4]/inoculum ratio of 30:6:3. Under these conditions, 90 and 85 % of the initial phenolic compounds and color were removed, respectively. High-pressure liquid chromatography analysis of extracts from treated and untreated OMW showed a clear and substantial reduction in phenolic compound concentrations. Phytotoxicity, assessed using radish (Raphanus sativus) seeds, indicated an increase in germination index of 23–92 % when a 30 % OMW concentration was treated with C. gallica in different dilutions (1/2, 1/4, and 1/8). Figure
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Sandra Vranic Ignacio Garcia-Verdugo Cécile Darnis Jean-Michel Sallenave Nicole Boggetto Francelyne Marano Sonja Boland Armelle Baeza-Squiban 《Environmental science and pollution research international》2013,20(5):2761-2770
Because of an increasing exposure to environmental and occupational nanoparticles (NPs), the potential risk of these materials for human health should be better assessed. Since one of the main routes of entry of NPs is via the lungs, it is of paramount importance to further characterize their impact on the respiratory system. Here, we have studied the uptake of fluorescently labeled SiO2 NPs (50 and 100 nm) by epithelial cells (NCI-H292) and alveolar macrophages (MHS) in the presence or absence of pulmonary surfactant. The quantification of NP uptake was performed by measuring cell-associated fluorescence using flow cytometry and spectrometric techniques in order to identify the most suitable methodology. Internalization was shown to be time and dose dependent, and differences in terms of uptake were noted between epithelial cells and macrophages. In the light of our observations, we conclude that flow cytometry is a more reliable technique for the study of NP internalization, and importantly, that the hydrophobic fraction of lung surfactant is critical for downregulating NP uptake in both cell types. 相似文献
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Christian Mougin Emmanuelle Artige Frédéric Marchand Samuel Mondy Céline Ratié Nadine Sellier Philippe Castagnone-Sereno Armelle Cœur D’Acier Daniel Esmenjaud Céline Faivre-Primot Laurent Granjon Valérie Hamelet Frederic Lange Sylvie Pagès Frédéric Rimet Nicolas Ris Guillaume Sallé 《Environmental science and pollution research international》2018,25(34):33849-33857
The Biological Resource Centre for the Environment BRC4Env is a network of Biological Resource Centres (BRCs) and collections whose leading objectives are to improve the visibility of genetic and biological resources maintained by its BRCs and collections and to facilitate their use by a large research community, from agriculture research to life sciences and environmental sciences. Its added value relies on sharing skills, harmonizing practices, triggering projects in comparative biology, and ultimately proposing a single-entry portal to facilitate access to documented samples, taking into account the partnership policies of research institutions as well as the legal frame which varies with the biological nature of resources. BRC4Env currently includes three BRCs: the Centre for Soil Genetic Resources of the platform GenoSol, in partnership with the European Conservatory of Soil Samples; the Egg Parasitoids Collection (EP-Coll); and the collection of ichthyological samples, Colisa. BRC4Env is also associated to several biological collections: microbial consortia (entomopathogenic bacteria, freshwater microalgae…), terrestrial arthropods, nematodes (plant parasitic, entomopathogenic, animal parasitic...), and small mammals. The BRCs and collections of BRC4Env are involved in partnership with academic scientists, as well as private companies, in the fields of medicinal mining, biocontrol, sustainable agriculture, and additional sectors. Moreover, the staff of the BRCs is involved in many training courses for students from French licence degree to Ph.D, engineers, as well as ongoing training. 相似文献