Mercuric chloride effects on adult rat oval cells-induced apoptosis

In the present investigation, the toxicity of mercuric chloride (HgCl₂) was evaluated in adult oval cells isolated from rat utilizing the 2-acetylaminofluorene/partial hepatectomy technique. Isolated oval cells were incubated with 5 μM of HgCl₂ for 8 hr to elucidate in vitro cytotoxic responses. Recently, autophagic cell death was found in rat hepatocytes in vitro within 30 min of incubation with 5 μM of mercury (Hg) which triggered apoptosis and necroptosis in a time-dependent manner. Nuclear degradation occurred within 30 min of incubation and progressed with time until 8 hr. Apoptosis evidenced by activation of caspase-dependent pathway between 30 min to 8 hr of incubation was mediated via interchange of death domain signaling pathways. Receptor-interacting protein played a positive role to modulate the death domain receptors in the scenario of apoptotic death of oval cells until 6 hr. Autophagic marker proteins ATG12 and LC3B exerted a significant role in triggering apoptosis in 5 μM Hg-treated oval cells. No apparent expression of apoptosis-inducing-factor (AIF) and HMGB1 indicated absence of caspase-independent apoptosis and necrosis in the rat oval cells between 30 min and 8 hr. Thus a low concentration of Hg modulates programmed cell death in adult rat oval cells by altering expression of proteins involved in the molecular mechanisms of cellular functions.     

Assessment of cropland inundation due to the operation of the Reelfoot Lake spillway in West Tennessee

Running Reelfoot Bayou (RRB) is the outlet canal of Reelfoot Lake, the largest natural lake in Tennessee. RRB is not able to contain discharge from Reelfoot Lake greater than the bankfull discharge of 28 m³/s (1000 ft³/s), which typically occurs at the beginning of the growing season (April–June). Historically, the planting of crops has been delayed until flooding subsides and cropland has drained. The objective of this study is a preliminary quantification of cropland inundation to determine its spatial distribution in the RRB floodplain. Inundated croplands in the RRB floodplain were delineated over a range of spillway discharges from 2 to 57 m³/s (70–2000 ft³/s), using one-dimensional–two-dimensional hydrodynamic modeling and multispectral satellite images (Landsat 8 and Sentinel-2). The composite maps made by combining the simulated and image-derived flood maps were overlaid on the United States Department of Agriculture CropScape layer to determine the inundation of individual summer crops during the growing season. About 25% of the inundated croplands are flooded at discharges of RRB less than 28 m³/s, implying wetland hydrology. The results of this analysis can be used to inform operational management of the Reelfoot Lake spillway.     

Interaction of plastic particles with heavy metals and the resulting toxicological impacts: a review

Environmental Science and Pollution Research - Interactions of plastic particles with different organic/inorganic pollutants including heavy metals impact their ecotoxicological potential, and...