Solid beef cattle manure application impacts on soil properties and 17β-estradiol fate in a clay loam soil

Livestock manure applied to agricultural land is one of the ways natural steroid estrogens enter soils. To examine the impact of long-term solid beef cattle (Bos Taurus) manure on soil properties and 17β-estradiol sorption and mineralization, this study utilized a soil that had received beef cattle manure over 35 years. The 17β-estradiol was strongly sorbed and sorption significantly increased (P < 0.05) with increasing soil organic carbon content (SOC) and with an increasing annual rate of beef cattle manure. The 17β-estradiol mineralization half-life was significantly negatively correlated, and the total amount of 17β-estradiol mineralized at 90 days (MAX) was significantly positively correlated with 17β-estradiol sorption. The long-term rate of manure application had no significant effect on MAX, but the addition of fresh beef cattle manure in the laboratory resulted in significantly (P < 0.05) smaller MAX values. None of the treatments showed MAX values exceeding one-third of the 17β-estradiol applied.     

Monitoring Australian Rangeland Sites Using Landscape Function Indicators and Ground- and Remote-Based Techniques

If the goal for managing rangelands is to achieve a balance between production and conservation, then monitoring is essential to detect change and apply corrective action. In some range-land areas of northern Australia, monitoring has detected a tilt in the production-conservation balance towards excessive production. How big is this imbalance? Can it shift back? Robust monitoring is needed to answer these questions. The aim is to know what to monitor, and where. For example, to detect changes caused by livestock on rangeland forage production and soil erosion, indicators linking grazing disturbances to landscape function are needed, that is, indicators that signal how well landscapes are capturing, concentrating, and utilizing scarce water, nutrient, and organic resources. Studies in Australia and the USA document that simple vegetation and soil patch attributes can be measured as indicators of the 'state of health' of landscape function. For example, field and remote sensing-based grazing studies in Australia document that landscapes with a high cover of perennial plant patches function effectively to capture runoff water and nutrients in sediments, whereas landscapes with a low cover of these patches do not — they are dysfunctional — as indicated by large patches of bare soil. Aerial videography is proving to be a robust technique for measuring indicators of landscape function such as small patches of vegetation and the extent of bare soil. These indicators typically have a sigmoidal response to grazing impacts. We illustrate that if these indicators are measured on monitoring sites established near the sigmoidal 'point of inflection’ then small changes in these indicators can be detected.     

Adverse effects in wild fish living downstream from pharmaceutical manufacture discharges

A set of biochemical and histological responses was measured in wild gudgeon collected upstream and downstream of urban and pharmaceutical manufacture effluents. These individual end-points were associated to fish assemblage characterisation. Responses of biotransformation enzymes, neurotoxicity and endocrine disruption biomarkers revealed contamination of investigated stream by a mixture of pollutants. Fish from sampled sites downstream of the industrial effluent exhibited also strong signs of endocrine disruption including vitellogenin induction, intersex and male-biased sex-ratio. These individual effects were associated to a decrease of density and a lack of sensitive fish species. This evidence supports the hypothesis that pharmaceutical compounds discharged in stream are involved in recorded endocrine disruption effects and fish population disturbances and threaten disappearance of resident fish species. Overall, this study gives argument for the utilisation of an effect-based monitoring approach to assess impacts of pharmaceutical manufacture discharges on wild fish populations.     

Assessment of patch quality by ladybirds: relative response to conspecific and heterospecific larval tracks a consequence of habitat similarity?

Summary. Aphid colonies can reach high levels of abundance but last for short periods of time. The larvae of aphidophagous ladybirds (Coleoptera: Coccinellidae) that feed on these colonies might therefore suffer from starvation, which favours the occurrence of cannibalism and intraguild predation. Thus, the assessment of patch quality becomes crucial and it has been shown that female ladybirds refrain from laying eggs in the presence of an oviposition deterring semiochemical deposited by their larvae. Adalia bipunctata (L.), Adalia decempunctata (L.) and Coccinella septempunctata L. are 3 sympatric species of ladybirds, which can co-occur in aphid colonies. As a consequence, their eggs and larvae are under threat, not only from cannibalism but also intraguild predation. Females should, therefore, also use the tracks deposited by heterospecific larvae to assess the quality of aphid colonies as oviposition sites. The expectation is that: 1- the strength of the reaction to each other’s larval tracks should be correlated with percentage habitat overlap and that 2- the reaction to conspecific larval tracks should be stronger than to heterospecific tracks. In order to test these hypotheses, females’ oviposition behaviour was analysed and a chemical analysis of the tracks of their larvae undertaken. The results show that oviposition behaviour is not related to habitat overlap. Both species of Adalia react to tracks of their own larvae and those of C. septempunctata, but A. decempunctata reacted more strongly than A. bipunctata. C. septempunctata reacted very slightly to its own tracks but not to those of either species of Adalia. The larval tracks are mainly composed of alkanes. Those of the two species of Adalia are qualitatively 100% similar and 60% so when the quantitative results are compared. They are, however, only 24–29% similar to those of Coccinella septempunctata.     

Defining patch mosaic functional types to predict invasion patterns in a forest landscape.

Alien plant invasions contribute significantly to global changes by often affecting biodiversity and ecosystem processes. Operational methods for identifying landscape attributes that promote or constrain plant invasions are urgently needed to predict their future spread and manage them efficiently. We combined landscape and functional ecology concepts to define patch mosaic functional types (PMFTs) as groups of cells showing the same response to a plant invasion in a heterogeneous forest landscape. The invasion of a European temperate forest by the American black cherry (Prunus serotina) has been chosen as a case study. A set of variables was collected, mapped using a Geographic Information System, and analyzed with multivariate analyses to correlate landscape traits with Prunus serotina abundance in each cell of a grid overlaid on the forest. A risk index was derived and mapped for three invasion levels: seedling colonization, tree establishment, and ecosystem invasion. Five PMFTs were identified and characterized by a set of traits related to soil properties, land use, disturbance, and invasion history. Scots pine plantations on podzols were the most invasible, while cells dominated by hydromorphic or calcareous soils were the most resistant. Most colonized patch mosaics provided suitable conditions for future establishment and invasion. Being strongly spatially connected, suitable patches provide corridors for Prunus serotina to colonize new parts of the forest. Conversely, the most resistant PMFTs were spatially agglomerated in the south of the forest and could act as a barrier. Colonization, establishment, and invasion risk maps were finally obtained by combining partial risks associated with each landscape trait at the cell scale. Within a heterogeneous landscape, we defined and organized PMFTs into a hierarchy, according to their associated risk for colonization, establishment, or invasion by a given invasive species. Each hierarchical level should be associated with a management strategy aiming at reducing one or more partial risk. Monitoring safe areas, extending cutting rotations, harvesting recently colonized stands tree by tree, promoting a multilayered understory vegetation, cutting down reproducing alien trees, favoring shade-tolerant, fast-growing, native tree species, removing alien trees at the leading edge, and proposing soil enrichment or irrigation in heavily invaded areas are recommended.     

Factors affecting male and female reproductive success in a chipmunk (Tamias sibiricus) with a scramble competition mating system

While sexual selection in mammals with female defense systems has historically received considerable attention, much less is known about the factors affecting reproductive success in mammalian species with scramble competition mating systems. Using mark–recapture techniques and DNA microsatellite loci to assign parentage, we examined the mating system and factors affecting the variation of the annual reproductive success in an introduced population of the Siberian chipmunk, Tamias sibiricus, a small, monomorphic, solitary squirrel. Our analyses showed that breeding females were spatially dispersed, setting the stage for a scramble competition mating system. Male reproductive success was positively associated with the size of the home range. The strength of sexual selection on this behavioral trait was very strong, equaling previous estimates for morphological traits in female defense mating systems. These findings suggest that a behavioral trait, space use, strongly influences the annual reproductive success in males, which is consistent with expectations in a scramble competition mating system. In both sexes, reproductive success was influenced by habitat, with twice as many juveniles produced in semi-open than in closed habitat, possibly due to differences in food availability between the two habitats.     

Sub-cellular partitioning of Cd, Cu and Zn in tissues of indigenous unionid bivalves living along a metal exposure gradient and links to metal-induced effects

We studied organ and sub-cellular distributions of several trace metals in a freshwater bivalve that has been proposed for use as a metal biomonitor. Specimens of Pyganodon grandis were collected from nine lakes located along a Cd, Cu and Zn concentration gradient (Rouyn-Noranda area, Quebec). Gills and digestive gland were isolated, homogenized and six sub-cellular fractions were separated by differential centrifugation and analysed for their Cd, Cu and Zn content. Metallothionein was quantified independently. Gill tissues contained abundant calcium concretions that accounted for over 60% of the total gill burden of each metal. Cadmium and Zn concentrations in this granule fraction reflected ambient metal concentrations. Metal concentrations in the digestive gland also responded to the metal contamination gradient, but to a lesser extent than the gills, reflecting the lower abundance of granules in the digestive gland. Metals (Cd, Cu) in this organ were present largely in the "heat-stable proteins" fraction, and metal concentrations in this fraction were strongly correlated with those of both metallothionein and, to a lesser extent, the "lysosomes+microsomes" and "mitochondria" fractions. In both organs, Cd concentrations in the "heat-denaturable protein" fraction remained low and constant, suggesting reasonably effective metal detoxification. Some evidence for oxidative stress was noted in the gills but not in the digestive gland. Overall, we conclude that in nature metals in P. grandis are bound differently in the gills and in the digestive gland and that metal detoxification in the former organ may be less effective than in the latter.