Production organique primaire dans un milieu saumâtre eutrophe (Etang de Berre). Effets d'une forte dilution (dérivation des eaux de la Durance)

¹⁴C primary production measurements were made over a period of 5 years (1965–1969, inclusive) in the brackish lake Etang de Berre, near Marseilles (France). The diversion of the River Durance into the Etang de Berre took place during this period (March 1966) and introduced an important modification into the organic production ecosystem, mainly through increased and variable freshening, accompanied by substantial nutrient input. The seasonal distribution of production rates displayed 3 bloom periods: the first (short and slight) in spring, the second (the most important as regards intensity and duration) in summer, and the third in autumn (October). Before the diversion of the river in 1965, the carbon-uptake rates in the lake ranged between 25 mg/m²/day in winter and 800 mg/m²/day in summer-autumn, the mean value for the year being 150 g C/m², which represents 2.5×10⁴ tons of photosynthesized carbon for the whole lake. After the diversion, more than 3000 mg C/m² day were measured; for 1968, the inclusive uptake rate was 384 g C/m², representing 6×10⁴ tons of synthesized carbon for the whole lake. Nevertheless, noticeable variations occurred from one year to another. From the annual nutrient input of phosphate to the Etang de Berre through the inflow of Durance waters, the quantity of potentially synthesizable elements has been calculated, in terms of carbon, according to the normal P:C ratio of organic substances; this quantity is called R. The difference between measured production, P, and R gives a measure of the regenerated production. This portion of production represented about 80% of the total production before 1968 but only 16% in 1969, a year of maximum fresh-water inflow. This phenomenon could be due to modifications of the ecophysiology of the phytoplankton resulting from the considerable freshening. With increasing nutrient load, eutrophication first occurs, then still greater dilution results in inhibition of production.     

Influence des conditions hivernales sur les productions phyto-et zooplanctoniques en Méditerranée Nord-Occidentale. III. Caractérisation des eaux de surface au moyen de cultures d'algues

Bioassays were performed on surface-water samples, collected during the Médiprod I cruise of the R.V. “Jean Charcot” in the northern part of the Mediterranean Sea. Physiological responses of axenic algal cultures were measured in order to characterize the different water masses. The responses obtained are specific to each algal strain: Phaeodactylum tricornutum grows in all waters sampled during the first part of the cruise, while Chaetoceros lauderi does not develop because of its different nutritional requirements; Chlamydomonas magnusii does not grow in any of the tested samples. No clear relationship exists between the results obtained in vitro and those obtained in situ but, on the whole, the waters collected during the second part of the cruise allow a higher growth of the test algae in vitro. This higher fertility is probably related to the algal bloom which occurred between the first and the second part of the cruise. In the authors' opinion the great difference in uptake rates observed between the high-turbulence area (low production) and the adjacent area (high production), may be related more to biological conditioning than to any physical process. However, physical mixing seems to be responsible for inhibition of primary productivity at the two stations where mixing attains maximum values.     

Influence des conditions hivernales sur les productions phyto- et zooplanctoniques en Méditerranée nord-occidentale. I. Structures hydrologiques et distribution des sels nutritifs

During two periods (1 to 15 March and 3 to 17 April, 1969), the R.V. “Jean Charcot” accomplished hydrological, chemical, and biological observations in the northern portion of the Western Mediterranean Sea. The main aim was to study the influence of the intensive vertical mixing of water masses on primary and secondary productions during winter. Earlier work in this area had revealed, in the offshore region, relatively large biomasses and high production rates during spring; considering the low potential fertility of Mediterranean waters, this finding may be explained by the effective vertical transport of nutrients upwards to the photic zone. The present paper discusses the hydrological features and the distribution of nutrients (phosphates and nitrates), in these areas, where, in winter, formation of deep water occurs. Observations were made down to 1,000 m on a grid of stations centred at 42°N; 5° E (“région provençale”) and another series of stations in the region “Corse-Côte d'Azur”. These two areas are characterized by cyclonic gyres, in which each centre shows a sector of high surface density (σ _t > 29.00). The surface mixed layer encountered is not deeper than 100 m; however, the Medoc Group (1970) has described formation of a homogeneous layer down to more than 1000 m. Many well-mixed water columns (maximum temperature and salinity at any depth do not exceed 13°C and 38.45‰, respectively) remain and occupy the western portion of the high surface density area; everywhere, the Mediterranean 3-layer system (superficial, intermediate and deep waters), more or less affected by mixing, remains well developed in the coastal region, and especially in the region of Côte d'Azur and Corsica. At some places, relatively high surface temperature (>13°C) associated with high salinity shows that the intermediate water fraction reaches the surface not only by direct vertical mixing, but also by any other dynamic lifting process, which constitutes another way of nutrient transport to the photic layer. The highest surface nutrient (0.3μ atg/l P?PO₄; 5 μ atg/l N?NO₃) and the lowest surface oxygen content (saturation <90%) are encountered in the mixed area; they are the best indicators of recently upwelled water. The surface layer around the cyclonic area is characterized by the low nutrient content of water of Atlantic origin. The lower salinity (<38%) of this water does not allow deep vertical mixing. During 3 to 17 April, 1969, the survey carried out on the same grid and sections reveals heating effects on all surface waters; the resulting stability in the most superficial layer accomodates the general spring bloom of phytoplankton. Nutrient consumption by photosynthesis in the upper 100 m layer of the surveyed area south of Marseilles was calculated to be: 129.1×10⁶ g-at P?PO₄ per 16,000 km² per month, converted on the atomic basis of P:C=1:106 represents almost 75% of the carbon production obtained by ¹⁴C-uptake measurements.     

Influence des conditions hivernales sur les productions phyto- et zooplanctoniques en Méditerranée Nord-Occidentale. II. Biomasse et production phytoplanctonique

Hydrological observations, and measurements of nutrient chemistry, plankton biomass, and production were carried out during the Médiprod I cruise of the R.V. “Jean-Charcot”. The March cruise was characterized by almost winter conditions, exhibiting strong vertical mixing of water masses in the offshore region and nutrient transport up to the photic zone. According to the working hypothesis, the strong vertically mixed area (e.g. Station 15) and the surrounding oligotrophic area are separated by an intermediate zone, where both nutrients and stability have produced phytoplankton-bloom conditions. During the April cruise, highest biomass and production rates were encountered everywhere in the offshore region, and especially in the previously mixed area of central divergence. Production was as high as 2 gC·m^-2 day^-1, and the standing crop of chlorophyll was 3 mg·m^-3; such values are rather important for the so called “poor” Mediterranean Sea. Salinity-phosphate and chlorophyll-phosphate diagrams are presented. Biomass and production rates are in agreement with the potential fertility based on the nutrient content of the waters. The disappearance of 1 μatg P·PO₄ by photosynthetic uptake corresponds to 7.7 mg chlorophyll a, which represents the autotrophic biomass remaining after grazing by the simultaneous zooplankton bloom. Biomass and production features are analyzed in regard to interaction of both nutrient availability and the stability of water masses. Stability conditions can be created either by intrusion of local mixing in a stratified oligotrophic area (“winter bloom”), or by thermal stratification of the upper layer (“spring bloom”). In the latter case, the highest biomasses are present in the zone where the nutrients were previously introduced by mixing. The oligotrophic situation remained constant during the two crunises in the surrounding coastal area, which is characterized by low-salinity water and, therefore, absence of vertical nutrient transport into the photic zone. Chlorophyll pigment concentration and photosynthetic rates in the “Cote d'Azur” region are similar to those in the “Provence” region; this situation may result more from upwelling of nutrient-rich intermediate water than from the mixing process which predominates in the latter region.     

Distribution et corrélations des principaux facteurs hydrobiologiques dans un milieu de forte production organique (Etang de Berre)

A comparison of biological and hydrographical factors was carried out in the surface waters of the Etang de Berre near Marseille. The following parameters were compared: primary production, chlorophyll a, seston, particulate carbon and albumin as biochemical indicators; temperature, salinity, oxygen, alcalinity and nutrients (phosphate, nitrate, nitrite and silicate) as physico-chemical parameters. Furthermore, quantitative and qualitative determinations of phytoplankton and zooplankton were made. During the investigations a negative correlation was found between the content of oxygen and the concentration of dissolved phosphorus. In the northern part of the area under investigation a vertical movement toward the surface exists, which can be explained by winds from the north (Mistral). The primary production and others biological factors are ten times more intensive than in the Mediterranean Sea. This fact may be due to eutrophication. In regard to the plankton, the diatoms —especially Nitzschia delicatissima CLEVE — are dominant. A relation between the percentage composition of phytoplankton and the copepod Acartia latisetosa was found.     

Concentrations of aliphatic and polycyclic aromatic hydrocarbons in ambient PM2.5 and PM10 particulates in Doha,Qatar

Organic carbon (OC), elemental carbon (EC), and 90 organic compounds (36 polycyclic aromatic hydrocarbons [PAHs], 25 n-alkane homologues, 17 hopanes, and 12 steranes) were concurrently quantified in atmospheric particulate matter of PM_2.5 and PM₁₀. The 24-hr PM samples were collected using Harvard Impactors at a suburban site in Doha, Qatar, from May to December 2015. The mass concentrations (mean ± standard deviation) of PM_2.5 and PM₁₀ were 40 ± 15 and 145 ± 70 µg m^?3, respectively, exceeding the World Health Organization (WHO) air quality guidelines. Coarse particles comprised 70% of PM₁₀. Total carbonaceous contents accounted for 14% of PM_2.5 and 10% of PM₁₀ particulate mass. The major fraction (90%) of EC was associated with the PM_2.5. In contrast, 70% of OC content was found in the PM_2.5–10 fraction. The secondary OC accounted for 60–68% of the total OC in both PM fractions, indicating photochemical conversions of organics are much active in the area due to higher air temperatures and solar radiations. Among the studied compounds, n-alkanes were the most abundant group, followed by PAHs, hopanes, and steranes. n-Alkanes from C₂₅ to C₃₅ prevailed with a predominance of odd carbon numbered congeners (C₂₇–C₃₁). High-molecular-weight PAHs (5–6 rings) also prevailed, within their class, with benzo[b + j]fluoranthene (Bb + jF) being the dominant member. PAHs were mainly (80%) associated with the PM_2.5 fraction. Local vehicular and fugitive emissions were predominant during low-speed southeasterly winds from urban areas, while remote petrogenic/biogenic emissions were particularly significant under prevailing northwesterly wind conditions.

Implications: An unprecedented study in Qatar established concentration profiles of EC, OC, and 90 organic compounds in PM_2.5 and PM₁₀. Multiple tracer organic compounds for each source can be used for convincing source apportionment. Particle concentrations exceeded WHO air quality guidelines for 82–96% of the time, revealing a severe problem of atmospheric PM in Doha. Dominance of EC and PAHs in fine particles signifies contributions from combustion sources. Dependence of pollutants concentrations on wind speed and direction suggests their significant temporal and spatial variability, indicating opportunities for improving the air quality by identifying sources of airborne contaminants.     

La distribution de l'oxygène en relation avec la production primaire en Méditerranée Nord-Occidentale

The distribution of oxygen and its seasonal variations in the upper layers of the Mediterranean Sea (northern part of the occidental basin) are analysed on the basis of hydrological features and in relation to phosphate distribution and data of ¹⁴C-uptake measurements. The well developed subsurface maximum of oxygen, associated with the summer thermocline, depends upon both physical and biological factors. The observed increase of oxygen through photosynthetic activity is maximal in the divergence zone. Biological oxygen content is calculated by applying the equation given by Redfield et al. (1963) after the oxygen and phosphate concentrations at various water depths have been computed, taking into account the proportions in which cold, winter water is mixed with water of the same depth and density. This is carried out by analysis of various diagrams (T?S, T?O₂, S?PO₄ and S?O₂). The results obtained by this procedure seem to be satisfactory, since the O₂ calculated on the basis of phosphate consumption (assuming a ΔO/ΔP ratio of-267:1) between winter and summer in the lower part of the photic zone is of the same order of magnitude as the observed oxygen increase. Low production, accumulation of the greatest part of the oxygen under the thermocline and supposed consumption by regeneration processes in the warm superficial water results in very low loss of oxygen into the atmosphere. The exchange rate of oxygen entering the water from the atmosphere can be expected to be intensive during a very cold winter, when the deep mixed layer results in undersaturation of the water surface. The yearly organic production by direct consumption of phosphates is about 1/4 of that obtained by the ¹⁴C-method. The concept of “new” and “regenerated” productions defined by Dugdale and Goering (1967) will be of use in future measurements of nitrogen uptake. The well recorded vertical mixing in the northern part of the western Mediterranean Basin which attains its maximum vertical and horizontal limits during extreme winter conditions, permits us to conclude that, in this region, the yearly balance of new production —more important for higher trophic levels — can be expected to be substantially higher than the yearly amount obtained when the preceding winter is as moderate as that of 1964. Production can be considered relatively high in regard to the low potential fertility of deep water (P?PO₄≈-0.4-0.5 μatg/l). The latter is, nevertheless, the highest of those of all Mediterranean regions; the divergence zone of the western basin seems to be the most fertile, if fluvial dillution zones are excepted.     

Influence des conditions hivernales sur les productions phyto- et zooplanctoniques en Méditerranée Nord-Occidentale. IV. Distribution verticale des taux d'adénosine triphosphate et de production primaire

This paper describes a method which utilizes ¹⁴C-labelled glucose for measuring the assimilation and mineralization of dissolved organic compounds in the sea. Incubation is carried out in 500 ml conic flasks, in darkness, at a temperature similar to that of the in-situ sampling levels. Direct trapping of CO₂ respired is performed in a scintillation vial, with an efficiency of 92% over a period of 4 h. The hyamin, utilized to trap the CO₂, excites the scintillating liquid in the presence of CO₂; the respiration data must then be corrected by a factor of 0.62. When HA Millipore filters are used for filtration, they quench assimilation values and the results must be corrected by a factor of 1.06. The reproducibility of the described method is good. Some parameters have been calculated: total heterotrophic activity, assimilation rate, respiration rate, bacterian yield, and the turn-over time of substrate in the water.