Enterotoxin production and antimicrobial susceptibility in Staphylococci isolated from traditional raw milk cheeses in Serbia

This study was undertaken to determine the prevalence of coagulase positive staphylococci (CPS) by examining a total of 71 raw milk cheeses. Additionally, enterotoxigenicity, antimicrobial susceptibility and the presence of mecA and mecC genes in the staphylococcal isolates were investigated. The isolation and enumeration procedure of CPS followed the International Organization for Standardization (ISO) standard. The presumptive staphylococci were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) using the VITEK MS system. VIDAS® Staph enterotoxin II assay was used for the detection of classical enterotoxins. Antimicrobial susceptibility testing (AST) was accomplished performing the disk diffusion method. All suspected methicillin resistant staphylococci were investigated for the presence of mecA and mecC genes by PCR assay. A high prevalence (87.32%) of CPS was detected in the cheeses at contamination levels up to 5.58 log CFU g^?1. Among 47 staphylococcal isolates screened for enterotoxin production, only one isolate, identified as S. hyicus, was confirmed as being enterotoxigenic. Resistance to penicillin (63.70%) was the most common resistance among the tested Staphylococcus aureus isolates. The dominant phenotypic resistance patterns in coagulase negative staphylococci (CNS) were resistance to ofloxacin and fusidic acid. All CNS isolates were susceptible to the clinically important antibiotics clindamycin, chloramphenicol, gentamicin, linezolid, rifampicin and trimethoprim-sulfamethoxazole. The mecA and mecC genes were not detected. To the best of our knowledge, this is the first study concerning evaluation of the presence of methicillin resistant staphylococci (MRS) in dairy products in Serbia.     

Quantification of present and past biomass productivity as a support to effective biomass management

We used statistical data including specific calculations to show how the productivity of different biomass categories varies with land use, environmental conditions and land management at national and local level in Slovakia. Three different study sites were selected. Averaged annual productivity of the total biomass from one hectare ranged from 5.96 to 12.03 t on arable land, from 4.02 to 15.20 t on grasslands and from 11.72 to 13.56 t on forest land. Although the biomass productivity varied among the various biomass categories and the study sites, the average annual values of the total biomass per hectare from productive land was about the same at all three study sites (11.62, 11.68, 11.78 t/ha). Detailed quantification of different biomass categories offered a variety of possibilities to use them for further applications in economy and ecosystem services. At the national level, the unused biomass could have produced 29.8 PJ (petajoule) of heat energy.