Prenatally detected double trisomy: Klinefelter and Down syndrome

Double trisomies are a rare occurrence. We report the first case of a Down and Klinefelter's syndrome (48,XXY,+21) in a fetus that was prenatally diagnosed during the 15th week of pregnancy. Even though the nasal bone was present, and the color-Doppler study of the ductus venosus and the nuchal thickness were normal, the maternal serum test results indicated an increased risk of Down syndrome and consequentially a genetic amniocentesis was performed. A 48,XXY,+21 karyotype was observed and the patient decided to terminate the pregnancy. In this case, we did not find the typical ultrasound (US) signs that would have led us to the chromosomopathy; furthermore, we emphasize the advantages of using biochemical screening which, in our case, were crucial in arriving at the correct diagnosis. Copyright © 2006 John Wiley & Sons, Ltd.     

Preliminary patterns of distribution and abundance of loggerhead sea turtles,<Emphasis Type="Italic"> Caretta caretta</Emphasis>, around Columbretes Islands Marine Reserve,Spanish Mediterranean

Aerial surveys were conducted to estimate the abundance and distribution of loggerhead turtles (Caretta caretta) in the Columbretes Islands Marine Reserve and surrounding waters (western Mediterranean). Four surveys were carried out during 2000 and 2001, following the line transect methodology. Loggerheads appeared to be present at high densities in the area throughout the whole year, although density varied between seasons, being more abundant during the spring. Mean density in the study area was 0.322 turtles/km² (range 0.200–0.516) and the mean abundance was 1,324 turtles (range 825–2,124). The turtles were distributed homogeneously throughout the study area, we found no difference in loggerhead density between the water around the reserve and that in the rest of the study area. Current conservation measures planned by the local authorities, which include increasing the area of the reserve, would be very positive for the conservation of this stock.Communicated by S.A. Poulet, Roscoff     

Follow-up trends of parasite community alteration in a marine fish after the Prestige oil-spill: shifting baselines?

This study evaluates the follow-up trends in the composition and structure of the parasite communities in the marine sparid Boops boops after the Prestige oil-spill. A total of 400 fish comprising 11 seasonal samples was analyzed from three impacted localities on the Atlantic coast of Spain. A large number of parasite species was recovered only after the spill thus suggesting a substantial alteration of the marine food webs. Post-spill communities exhibited higher richness and abundance due to the significant changes in the abundance of the common species, the latter indicating accelerated parasite transmission rates. Multivariate analyses at two nested scales detected a directional trend in parasite community succession towards the pre-spill situation, however, with no full support for community recovery. The state of parasite communities in 2005-2006 may provide the new baseline data which can serve as a framework for quantifying the impact of potential future spills in the region.     

Relations between PM10 composition and cell toxicity: a multivariate and graphical approach

Previous studies have used particle mass and size as metrics to link airborne particles with deleterious health effects. Recent evidence suggests that particle composition can play an important role in PM-toxicity; however, little is known about the specific participation of components (individually or acting in groups) present in such a complex mixture that accounts for toxicity. This work explores relationships among PM(10) components in order to identify their covariant structure and how they vary in three sites in Mexico City. Relationships between PM(10) with cell toxicity and geographical location were also explored. PM(10) was analyzed for elemental composition, organic and elemental carbon, endotoxins and the induction of inhibition of cell proliferation, IL-6, TNFalpha and p53. PM(10) variables were evaluated with principal component analysis and one-way ANOVA. The inhibition of cell proliferation, IL-6 and TNFalpha were evaluated with factorial ANOVA and p53 with the Welch test. The results indicate that there is heterogeneity in particle mass, composition and toxicity in samples collected at different sites. Multivariate analysis identified three major groups: (1) S/K/Ca/Ti/Mn/Fe/Zn/Pb; (2) Cl/Cr/Ni/Cu; and (3) endotoxins, organic and elemental carbon. Groups 1 and 3 showed significant differences among sites. Factorial ANOVA modeling indicated that cell proliferation was affected by PM concentration; TNFalpha and IL-6 by the interaction of concentration and site, and p53 was different by site. Radial plots suggest the existence of complex interactions between components, resulting in characteristic patterns of toxicity by site. We conclude that interactions of PM(10) components determine specific cellular outcomes.     

Landfill aeration in the framework of a reclamation project in Northern Italy

In situ aeration by means of the Airflow technology was proposed for landfill conditioning before landfill mining in the framework of a reclamation project in Northern Italy. A 1-year aeration project was carried out on part of the landfill with the objective of evaluating the effectiveness of the Airflow technology for landfill aerobization, the evolution of waste biological stability during aeration and the effects on leachate and biogas quality and emissions.The main outcomes of the 1-year aeration project are presented in the paper.The beneficial effect of the aeration on waste biological stability was clear (63% reduction of the respiration index); however, the effectiveness of aeration on the lower part of the landfill is questionable, due to the limited potential for air migration into the leachate saturated layers.During the 1-year in situ aeration project approx. 275 MgC were discharged from the landfill body with the extracted gas, corresponding to 4.6 gC/kgDM. However, due to the presence of anaerobic niches in the aerated landfill, approx. 46% of this amount was extracted as CH₄, which is higher than reported in other aeration projects. The O₂ conversion quota was lower than reported in other similar projects, mainly due to the higher air flow rates applied.The results obtained enabled valuable recommendations to be made for the subsequent application of the Airflow technology to the whole landfill.     

The PAF model: an integrated approach for landfill sustainability

Lab-scale tests were set up to investigate different options for achieving sustainability by reducing long-term landfill emissions. The options which have been studied and compared with the traditional anaerobic landfill for unprocessed refuse were: mechanical-biological pretreatment, landfill aeration with forced and with natural advective air flow (semi-aerobic), flushing. Combination of different options have been experimented. The combination of mechanical-biological Pretreatment, Aeration by the semi-aerobic method and Flushing (PAF model) seems to synergize the advantages of the individual options. The research is ongoing and further studies are necessary in order to assess the full-scale applicability of the model.     

Tests for the evaluation of ammonium attenuation in MSW landfill leachate by adsorption into bentonite in a landfill liner

Uncontrolled leachate emissions are one of the key factors in the environmental impact of municipal solid waste (MSW) landfills. The concentration of ammonium, given the anaerobic conditions in traditional landfills, can remain significantly high for a very long period of time, as degradation does not take place and volatilisation is not significant (the pH is not high enough to considerably shift the equilibrium towards un-ionised ammonia). Recent years have witnessed a continuous enhancement of landfill technology in order to minimize uncontrolled emissions into the environment; bottom lining systems have been improved and more attention has been devoted to the study of the attenuation of the different chemicals in leachate in case of migration through the mineral barrier. Different natural materials have been considered for use as components of landfill liners in the last years and tested in order to evaluate the performance of the different alternatives. Among those materials, bentonite is often used, coupled with other materials in two different ways: in addition to in situ soil or in geocomposite clay liner (GCL). A lab-scale test was carried out in order to further investigate the influence of bentonite on the attenuation of ammonium in leachate passing through a landfill liner. Two different tests were conducted: a standardized batch test with pulverized bentonite and a batch test with compacted bentonite. The latter was proposed in order to better simulate the real conditions in a landfill liner. The two tests produced values for the partition coefficient K(d) higher than the average measured for other natural materials usually utilized as components of landfill liners. Moreover, the two tests showed similar results, thus providing a further validation of the suitability of the standard batch test with pulverized bentonite. A thorough knowledge of attenuation processes of ammonium in landfill liners is the basis for the application of risk analysis models for the evaluation of the failure of bottom liners or their components.     

Fine-scale distribution of juvenile Atlantic and Mediterranean loggerhead turtles (Caretta caretta) in the Mediterranean Sea

Loggerhead turtles nesting in the Mediterranean Sea exhibit remarkable genetic structuring. This paper tests the hypothesis that young loggerhead turtles from different rookeries do not distribute homogeneously among the major Mediterranean foraging grounds, due to a complex pattern of surface currents. We extracted long fragments of mitochondrial DNA from 275 stranded or bycaught juvenile turtles from six foraging grounds (Catalano-Balearic Sea, Algerian basin, Tyrrhenian Sea, Adriatic Sea, northern Ionian Sea and southern Levantine Sea). We used a Bayesian mixed-stock analysis to estimate the contributions from rookeries in the Mediterranean, the North-west Atlantic and Cape Verde to the studied foraging grounds. Differences were found in the relative contribution of juvenile turtles of Atlantic and Mediterranean origin to each foraging ground. A decreasing proportion of Atlantic juveniles was detected along the main surface current entering the Mediterranean, with a high prevalence of turtles from eastern Florida in the Algerian basin and lower numbers elsewhere. In regard to the turtles of Mediterranean origin, juveniles from Libya prevailed in central and western Mediterranean foraging grounds other than the Algerian basin. Conversely, the Adriatic Sea was characterised by a large presence of individuals from western Greece, while the southern Levantine Sea was inhabited by a heterogeneous mix of turtles from the eastern Mediterranean rookeries (Turkey, Lebanon and Israel). Overall, the distribution of juveniles may be related to surface circulation patterns in the Mediterranean and suggests that fisheries might have differential effects on each population depending on the overlap degree between foraging and fishing grounds.     

Use of the Contingent Valuation Method in the assessment of a landfill mining project

A comprehensive approach for the evaluation of the economic feasibility of landfill mining (LFM) should take into account not only the direct costs and revenues for the private investor, but also the social benefits or costs (generally called externalities), in such a way that projects generating major social benefits (and no significant private revenues) are not overlooked.With a view to contributing to the development of a common framework for the evaluation of LFM projects, this paper presents the results of a case study where the issue of the assessment of social benefits from a LFM project is addressed. In particular, the Contingent Valuation Method is applied for the monetary assessment of the community-perceived benefits from the remediation of an old uncontrolled waste deposit by means of LFM and the conversion of the area into a park.Based on the results of a survey carried out on a random sample of people living near the old landfill, the economic values of the individual willingness to pay (WTP) for LFM and the subsequent creation of a public park were calculated and the correlations with the relevant variables (distance from the landfill site, age, income, sex, education level) assessed. The results were then suitably extended and the monetary value of the welfare increase of the whole population resident in the area and potentially affected both by LFM and the creation of the park was calculated.