Biochemical responses,growth and reproduction of earthworm in low density polyethylene (LDPE)

There is an unprecedented production of plastic that is accelerating its disposal while affecting the fitness of the terrestrial as well as the aquatic environment. The term microplastics refers to plastic fragments that are less than 5 mm in size and are widely distributed in the environment. Therefore, the present study intends to explore the biological response of earthworms (Eisenia fetida) toward different concentrations of low-density polyethylene. E. fetida treated with low-density polyethylene concentration (Control), 250 mg kg⁻¹, 1000 mg kg⁻¹, 6000 mg kg⁻¹, 12,000 mg kg^−1, and 25,000 mg kg⁻¹. The above ratios were thoroughly mixed with 1kg of artificial soil and tested for growth, reproduction (cocoons and hatchling count), and enzymatic activities namely superoxide dismutase, guaiacol peroxidase, glutathione-S-transferase, and glutathione reductase and molecular docking studies. No mortality was observed during the exposure period at any concentrations. On the 28th day, when compared to the control the highest decrease in body weight of earthworms was observed in 25,000 mg (28.4%) followed by 12,000 mg (12.2%) and 6000 mg (3.4%). The cocoon and hatchlings significantly declined as the dose of microplastics increases. Enzymatic activity such as SOD and POD showed declined trend as the dose increased, while GST and GR increased with an increase in microplastic concentrations on 28th day. Furthermore, molecular docking showed that LDPE can modulate the activity of all four enzymes significantly.     

Earthworms,pesticides and sustainable agriculture: a review

The aim of this review is to generate awareness and understand the importance of earthworms in sustainable agriculture and effect of pesticides on their action. The natural resources are finite and highly prone to degradation by the misuse of land and mismanagement of soil. The world is in utter need of a healthy ecosystem that provides with fertile soil, clean water, food and other natural resources. Anthropogenic activities have led to an increased contamination of land. The intensification of industrial and agricultural practices chiefly the utilization of pesticides has in almost every way made our natural resources concave. Earthworms help in a number of tasks that support many ecosystem services that favor agrosystem sustainability but are degraded by exhaustive practices such as the use of pesticides. The present review assesses the response of earthworm toward the pesticides and also evaluates the relationship between earthworm activity and plant growth. We strictly need to refresh and rethink on the policies and norms devised by us on sustainable ecology. In an equivalent way, the natural resources should be utilized and further, essential ways for betterment of present and future livelihood should be sought.     

Extraction of earthworm from soil by different sampling methods: a review

Earthworms are so closely incorporated into the soil structure that they are not calculated easily from the soil. Due to this property, their extraction from soil is tedious and time-consuming. Different methods have been used for their extraction from the soil, and efficiency of each method is affected by physical properties, viz., temperature and moisture of the soil. This study explored the advantage and disadvantage of different sampling methods for the extraction of earthworms such as hand sorting, octet method, formalin method, mustard extraction method, allyl isothiocyanate (AITC), and onion extraction method. Extraction efficiency of formalin is 20–60 times more as compared to hand sorting, but hand sorting gives satisfactory results for earthworms of more than 0.2 g live weight, and cocoons can also be recovered by this method. Octet method is effective in extracting anecic species and could easily be applied to site where chemical extraction is not a viable option. Extraction by mustard and AITC is simple, low cost, and more efficient for the extraction of deep-burrowing anecic species. The onion extraction solution is low cost and nontoxic which can be used as alternative to formalin. Like mustard, the onion solution is also inexpensive and not harmful to the earthworms and environment.     

Comparing the nutrient changes,heavy metals,and genotoxicity assessment before and after vermicomposting of thermal fly ash using Eisenia fetida

Environmental Science and Pollution Research - Fly ash (FA) is available in an unstable state and can be ameliorated by vermicomposting. The different ratios of FA viz (FA10, FA15, FA20, FA25,...