Characterization of ACC deaminase-producing endophytic bacteria isolated from copper-tolerant plants and their potential in promoting the growth and copper accumulation of Brassica napus

One hundred Cu-resistant-endophytic bacteria were isolated from Cu-tolerant plants grown on Cu mine wasteland, of which, eight Cu-resistant and 1-aminocyclopropane-1-carboxylate (ACC) deaminase-producing endophytic bacteria were obtained based on the ACC deaminase activity of the bacteria and characterized with respect to metal resistance, production of ACC deaminase, indole-3-acetic acid (IAA) as well as siderophores and mineral phosphate solubilization. Ralstonia sp. J1-22-2, Pantoea agglomerans Jp3-3, and Pseudomonas thivervalensis Y1-3-9 with higher ACC deaminase activity (ranging from 213 to 370 μM α-ketobutyrate mg(-1)h(-1)) were evaluated for promoting plant growth and Cu uptake of rape grown in quartz sand containing 0, 2.5, and 5 mg kg(-1) of Cu in pot experiments. The eight bacteria were found to exhibit different multiple heavy metal resistance characteristics, to show different levels of ACC deaminase activity and to produce indole acetic acid. Seven bacteria produced siderophores and solubilized inorganic phosphate. Pot experiments showed that inoculation with the strains (J1-22-2, Jp3-3, and Y1-3-9) was found to increase the biomass of rape. Increases in above-ground tissue Cu contents of rape cultivated in 2.5 and 5 mg kg(-1) of Cu-contaminated substrates varied from 9% to 31% and from 3 to 4-fold respectively in inoculated-rape plants compared to the uninoculated control. The maximum Cu uptake of rape was observed after inoculation with P. agglomerans Jp3-3. The results show that metal-resistant and plant growth promoting endophytic bacteria play an important role in plant growth and Cu uptake which may provide a new endophytic bacterial-assisted phytoremediation of Cu-contaminated environment.     

Low ambient temperature increases hospital re-admissions for systemic lupus erythematosus in humid subtropical region: a time series study

Currently, the correlation between ambient temperature and systemic lupus erythematosus (SLE) hospital admissions remains not determined. The aim of this study was to explore the correlation between ambient temperature and SLE hospital admissions in Hefei City, China. An ecological study design was adopted. Daily data on SLE hospital admissions in Hefei City, from January 1, 2007, to December 31, 2017, were obtained from the two largest tertiary hospitals in Hefei, and the daily meteorological data at the same period were retrieved from China Meteorological Data Network. The generalized additive model (GAM) combined with distributed lag nonlinear model (DLNM) with Poisson link was applied to evaluate the influence of ambient temperature on SLE hospital admissions after controlling for potential confounding factors, including seasonality, relative humidity, day of week, and long-term trend. There were 1658 SLE hospital admissions from 2007 to 2017, including 370 first admissions and 1192 re-admissions (there were 96 admissions with admission status not stated). No correlation was observed between ambient temperature and SLE first admissions, but a correlation was found between low ambient temperature and SLE re-admissions (RR: 2.53, 95% CI: 1.11, 5.77) (3.5 °C vs 21 °C). The effect of ambient temperature on SLE re-admissions remained for 2 weeks but disappeared in 3 weeks. Exposure to low ambient temperature may increase hospital re-admissions for SLE, and thus it is important for SLE patients to maintain a warm living environment and avoid exposure to lower ambient temperature.

     

Germinable Soil Seed Banks and the Restoration Potential of Abandoned Cropland on the Chinese Hilly-Gullied Loess Plateau

Poor vegetation cover is generally considered to be a major factor causing soil erosion on the Loess Plateau in China. It has been argued that tree planting restoration is ineffective, and natural re-vegetation is an alternative ecological solution for restoring abandoned cropland and controlling soil erosion. The aims of this study were to investigate the characteristics of soil seed banks and to assess the natural restoration potential of abandoned cropland in the hilly-gullied Loess Plateau. The soil seed bank was identified by the germination method with the soil samples, which were collected at four sampling times (April, August, and October 2005 and August 2006) from 12 plots abandoned 3–30 years prior to sampling. The seed bank densities of all of the samples in the 0–10 cm soil layer varied from 1,067 ± 225 to 14,967 ± 1,606 seeds m⁻². Fifty-one species (24 annual and 27 perennial species) belonging to 18 families were identified, and 39% of these species belonged to the families Compositae and Gramineae. The pioneer species Artemisia scoparia dominated the seed bank, with an average seed density of 3,722 seeds m⁻², and accounted for 74.4% of the seeds in the bank. The local dominant species (such as Lespedeza davurica, Artemisia gmelinii, Bothriochloa ischaemun and Stipa bungeana) of the later succession stages also existed at densities varying from 17 to 1, 383 seeds m⁻². The combination of soil seed bank characteristics, reproductive traits of the species, the specific landscape conditions indicates that the potential to restoring the abandoned croplands in the hilly-gullied Loess Plateau via natural re-vegetation could be substantial.