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The most common mutation in alpha-1-antitrypsin deficiency, conversion of a G to an A at base 9989 (PI-Z), was detected with the chemical cleavage of mismatch method, demonstrating the power of the method for prenatal diagnosis. Exon V of the gene was amplified using the polymerase chain reaction and heteroduplexes were formed to test for the presence of the mutation. The predicted C mismatch was readily detectable with hydroxylamine, and by making the probe from the chorionic villus sample it was possible to determine that the fetus was heterozygous, not homozygous, for the mutation. 相似文献
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Joseph Forrest Leonard Newman 《Journal of the Air & Waste Management Association (1995)》2013,63(9):761-768
The role of sulfur compounds in our environment has been the subject of much speculation during the past two decades. An evaluation of the effect of man-made contributions to the biogeochemical sulfur cycle requires a comprehensive examination of source magnitudes, atmospheric concentrations and removal processes. Many voids exist in our present knowledge of these parameters despite the contributions of numerous researchers. Adequate information is needed concerning the various forms of sulfur as well as other critical constituents which exist in our biosphere so that their interrelationship and role in the mechanisms of the sulfur cycle may be more fully understood. 相似文献
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B. Miller # R. Reading J. Hoogland † T. Clark ‡ G. Ceballos § R. List S. Forrest †† L. Hanebury ‡‡ P. Manzano § J. Pacheco § and D. Uresk§§ 《Conservation biology》2000,14(1):318-321
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Forrest E. Dierberg 《Environmental management》1992,16(3):371-380
Following a period of prolonged drought or intentional lake level drawdown, large littoral areas that once contained submersed
aquatic vegetation (SAV) are reinundated when lake levels rise. A complete assessment of the contribution made by decomposing
SAV to the in-lake phosphorus (P) concentration is important in both the management of Lake Okeechobee and understanding basic
P processes. The P contribution to the open waters of Lake Okeechobee from a rapid inundation of exposed SAV was calculated
by four methods: cores of field-desiccated SAV, cores of lab-desiccated SAV in the presence and absence of sediments, in situ
decomposition, and sequential macrophyte harvesting. P releases, given such an episodic event, were similar among the four
methods, ranging from 116±48 to 384±528 mg/m2 in the absence of sediment. When SAV is in contact with sediment, which is the realistic field situation, the amount of P
released was four times less (30±14 mg/m2) than in the absence of sediment. The calculated P releases would result in total P concentration increases in the lake from
2 to 15 μg/liter (upper 95% CI=2–25 μg/liter) in the absence of sediment; only 1 μg/liter increase was predicted when SAV
released P in contact with sediment. Thus it is unlikely that a significant rise in total P concentrations in the limnetic
zone of the lake would occur from the export of P released during the desiccation of SAV in the littoral-marsh zone during
a drawdown. 相似文献
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- Cleanup of a contaminated site is halted as community residents launch a legal challenge against the method that those responsible for the site have selected for remediation.
- Parents and teachers at a public school call for an investigation to determine if substances in the building are making students and staff sick. Midway into the investigation, they also become concerned as to whether the right tests are being performed to detect contamination that may be present, and how they will know what the test results mean.
- Industrial neighbors of a facility oppose its air permit application out of concern for the effects the facility's emissions might have on their own workers.
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